Abstract
Ribose-phosphate pyrophosphokinase 1 (PRPS1) was identified and isolated as a differentially expressed gene between deltamethrin-susceptible (DS) and deltamethrin-resistant (DR) Culex pipiens pallens and Aedes albopictus C6/36 cell line through microarray and 2D-Gel. An open reading frame of PRPS1 cloned from C. pipiens pallens has 1,011 bp and encodes for a 336 amino acids protein which shares high homology with Culex quinquefasciatus. Real-time polymerase chain reaction was used to determine the transcript expression level of PRPS1 in DS and DR strains. The expression levels of PRPS1 were higher in DR laboratory strains and natural population JXZ-DR, JXZ-LDR. PRPS1 was also detected and expressed at all developmental stages of C. pipiens pallens and increased expression level in DR3 strain than DS strain in the third and fourth instar larvae, female and male stages. In addition, to further investigate the role of PRPS1 in deltamethrin resistance, PRPS1 was transiently expressed in A. albopictus C6/36 cells and detected by western blotting. Cells transfected with PRPS1 had an increased resistance to deltamethrin compared with control cells. These results suggested that the increased expression level of PRPS1 may play roles in the regulation of deltamethrin resistance.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barlow SM, Sullivan FM et al (2001) Risk assessment of the use of deltamethrin on bednets for the prevention of malaria. Food Chem Toxicol 39(5):407–422
Burton MJ, Mellor IR et al (2011) Differential resistance of insect sodium channels with kdr mutations to deltamethrin, permethrin and DDT. Insect Biochem Mol Biol 41(9):723–732
Chen L, Zhong D et al (2010) Molecular ecology of pyrethroid knockdown resistance in Culex pipiens pallens mosquitoes. PLoS One 5(7):e11681
Foster SP, Gorman K et al (2010) English field samples of Thrips tabaci show strong and ubiquitous resistance to deltamethrin. Pest Manag Sci 66(8):861–864
Gong MQ, Gu Y et al (2005) Cloning and overexpression of CYP6F1, a cytochrome P450 gene, from deltamethrin-resistant Culex pipiens pallens. Acta Biochim Biophys Sin (Shanghai) 37(5):317–326
Hammon WM, Rees DM et al (1949) Experimental transmission of Japanese B encephalitis virus by Culex tritaeniorhynchus and Culex pipiens var. pallens, suspected natural vectors. Am J Hyg 50(1):46–50
Hemingway J, Field L et al (2002) An overview of insecticide resistance. Science 298(5591):96–97
Hemingway J, Hawkes NJ et al (2004) The molecular basis of insecticide resistance in mosquitoes. Insect Biochem Mol Biol 34(7):653–665
Jinfu W (1999) Resistance to deltamethrin in Culex pipiens pallens (Diptera: Culicidae) from Zhejiang, China. J Med Entomol 36(3):389–393
Kozak M (1986) Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44(2):283–292
Livak, K.J. and Schmittgen, T.D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method, Methods, 25, 402–408
Matambo TS, Abdalla H et al (2007) Insecticide resistance in the malarial mosquito Anopheles arabiensis and association with the kdr mutation. Med Vet Entomol 21(1):97–102
Mattingly PF (1962) Some considerations relating to the role of Culex pipiens fatigans Wiedemann in the transmission of human filariasis. Bull World Health Organ 27:569–578
Montella IR, Martins AJ et al (2007) Insecticide resistance mechanisms of Brazilian Aedes aegypti populations from 2001 to 2004. Am J Trop Med Hyg 77(3):467–477
Omori N (1958) Experimental studies on the role on the house mosquito, Culex pipiens pallens in the transmission of bancroftian filariasis. III. Duration of life of filariae in mosquitoes exposed to winter temperatures. Yokohama Med Bull 9(6):382–390
Pasteur N, Raymond M (1996) Insecticide resistance genes in mosquitoes: their mutations, migration, and selection in field populations. J Hered 87(6):444–449
Pedra JH, McIntyre LM et al (2004) Genome-wide transcription profile of field- and laboratory-selected dichlorodiphenyltrichloroethane (DDT)-resistant Drosophila. Proc Natl Acad Sci U S A 101(18):7034–7039
Roessler BJ, Bell G et al (1990) Cloning of two distinct copies of human phosphoribosylpyrophosphate synthetase cDNA. Nucleic Acids Res 18(1):193
Soderlund DM, Knipple DC (2003) The molecular biology of knockdown resistance to pyrethroid insecticides. Insect Biochem Mol Biol 33(6):563–577
Sun H, Sun L et al (2011) Cloning and characterization of ribosomal protein S29, a deltamethrin resistance associated gene from Culex pipiens pallens. Parasitol Res 109(6):1689–1697
Sun L, Qian J et al (2007) Continuous, mixed, and alternating exposure to deltamethrin and fenthion affect development of resistance in Culex pipiens pallens in China. J Am Mosq Control Assoc 23(3):330–334
Vais H, Williamson MS et al (2001) The molecular interactions of pyrethroid insecticides with insect and mammalian sodium channels. Pest Manag Sci 57(10):877–888
Vontas J, Blass C et al (2005) Gene expression in insecticide resistant and susceptible Anopheles gambiae strains constitutively or after insecticide exposure. Insect Mol Biol 14(5):509–521
Yang Q, Zhou D et al (2008) Expression and characterization of two pesticide resistance-associated serine protease genes (NYD-tr and NYD-ch) from Culex pipiens pallens for metabolism of deltamethrin. Parasitol Res 103(3):507–516
Yoon KS, Kwon DH et al (2008) Biochemical and molecular analysis of deltamethrin resistance in the common bed bug (Hemiptera: Cimicidae). J Med Entomol 45(6):1092–1101
Zhang J, Yang M et al (2011) prag01, a novel deltamethrin-resistance-associated gene from Culex pipiens pallens. Parasitol Res 108(2):417–423
Zhang Z, Yang C (1996) Application of deltamethrin-impregnated bednets for mosquito and malaria control in Yunnan, China. Southeast Asian J Trop Med Public Health 27(2):367–371
Acknowledgments
This work was supported by the National Institutes of Health of USA (NIH; Grant No. 2R01AI075746-05), the National Natural Science Foundation of China (Grant No. 30901244, 30972564, 81171900, and 81101279), the National S & T Major Program (Grant No. 2012ZX10004-219 and 2008ZX10004-010), the National S & T Major Program (Grant No. 2012ZX10004-220 and 2008ZX10004-011), Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No.20113234120007), Natural Science Foundation of Jiangsu Province (Grant No.81101279), and Priority Academic Program Development of Jiangsu Higher Education Institutions.
Author information
Authors and Affiliations
Corresponding authors
Electronic supplementary material
Below is the link to the electronic supplementary material.
ESM 1
(DOC 39 kb)
Rights and permissions
About this article
Cite this article
Hong, S., Zhou, D., Chen, C. et al. Ribose-phosphate pyrophosphokinase 1 (PRPS1) associated with deltamethrin resistance in Culex pipiens pallens . Parasitol Res 112, 847–854 (2013). https://doi.org/10.1007/s00436-012-3205-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-012-3205-2