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Larvicidal activity of lansiumamide B from the seeds of Clausena lansium against Aedes albopictus (Diptera: Culicidae)

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Abstract

The larvicidal activity of crude petroleum ether, toluene, n-butanol, ethyl acetate, acetone, and methanol extracts of the seeds of Clausena lansium was assayed for their toxicities against the early fourth instar larvae of Aedes albopictus. The larval mortality was observed after 24-h exposure. The LC50 value of petroleum ether extract was 22.99 ppm, showing the best larvicidal activity among all six solvent extracts. A cinnamon amide compound lansiumamide B (N-methyl-N-cis-styrylcinnamamide) was isolated from the petroleum ether extract by column chromatographic method, which exhibited a strong larvicidal activity against the early fourth instar larvae of A. albopictus with LC50 and LC90 values of 0.45 and 2.19 ppm, respectively. The structure was elucidated by 1H NMR, 13C NMR spectral data. The larvicidal activity against mosquito of lansiumamide B from the seed of C. lansium was evaluated for the first time.

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Acknowledgments

This work was supported by the Science and Technology Planning Project of Guangzhou (2010Y1-27). The authors are thankful to the Guangzhou Center for Disease Control and Prevention for providing the larvae of mosquito.

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  1. Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Wushan, Guangzhou, 510642, People’s Republic of China

    Yun Han, Li-chun Li, Wen-bo Hao, Ming Tang & Shu-qing Wan

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  1. Yun Han
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  2. Li-chun Li
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Correspondence to Shu-qing Wan.

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Han, Y., Li, Lc., Hao, Wb. et al. Larvicidal activity of lansiumamide B from the seeds of Clausena lansium against Aedes albopictus (Diptera: Culicidae). Parasitol Res 112, 511–516 (2013). https://doi.org/10.1007/s00436-012-3161-x

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