Abstract
Angiostrongylus cantonensis is a rodent nematode. Adult worms of A. cantonensis live in the pulmonary arteries of rats. Humans and mice are accidental hosts or named nonpermissive hosts. The larva cannot develop into an adult worm and only causes serious eosinophilic meningitis or meningoencephalitis if humans or mice eat food containing larva of A. cantonensis in the third stage. The differing consequences largely depend on differing immune responses of the host to parasite during A. cantonensis invasion and development. Microglia is considered to be the key immune cell in the central nervous system like macrophage. To further understand the reasons for why mice and rats attain different outcomes in A. cantonensis infection, we set up the method to isolate and culture newborn rats’ primary microglia and observe the activation of the microglia cells, comparing with mice microglia cell line N9. We treated cells with soluble antigen of the fourth larva of A. cantonensis (L4 larva) and measured mRNA levels of IL-1β, IL-5, IL-6, IL-13, eotaxin, iNOS, and TNF-α by real-time PCR. The results showed that N9 expressed high mRNA level of IL-6, IL-1β, TNF-α, iNOS, IL-5, IL-13, and eotaxin, but primary microglia only had IL-5, IL-13, and eotaxin mRNA level. It implies that microglia from rats and mice had different reaction to soluble antigen of A. cantonensis. Therefore, we supposed that microglia may play an immune modulation role during the brain inflammation induced by A. cantonensis.
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References
Bhardwaj N, Ghaffari G (2012) Biomarkers for eosinophilic esophagitis: a review. Ann Allergy Asthma Immunol 109(3):155–159
Chen HT (1935) Un nouveau nematoda pulmonaire: Pulmonema cantonensis, n. g., n. sp. des rats de Canton. Ann Parasitol Hum Comp 13:312–317
Diao Z, Chen X, Yin C, Wang J, Qi H, Ji A (2009) Angiostrongylus cantonensis: effect of combination therapy with albendazole and dexamethasone on Th cytokine gene expression in PBMC from patients with eosinophilic meningitis. Exp Parasitol 123(1):1–5
Eamsobhana P, Yoolek A, Punthuprapasa P, Yong HS (2009) Thai Koi-Hoi snail dish and angiostrongyliasis due to Angiostrongylus cantonensis: effects of food flavoring and alcoholic drink on the third-stage larvae in infected snail meat. Foodborne Pathog Dis 6:401–405
Garden GA, Möller T (2006) Microglia biology in health and disease. J Neuroimmune Pharmacol 1:127–137
Henry CJ, Huang Y, Wynne AM, Godbout JP (2009) Peripheral lipopolysaccharide (LPS) challenge promotes microglial hyperactivity in aged mice that is associated with exaggerated induction of both pro-inflammatory IL-1beta and anti-inflammatory IL-10 cytokines. Brain Behav Immun 23(3):309–317
Heppner FL, Greter M, Marino D (2005) Experimental autoimmune encephalomyelitis repressed by microglial paralysis. Nat Med 11:146–152
Hu X, Li JH, Lan L, Wu FF, Zhang EP, Song ZM, Huang HC, Luo FJ, Pan CW, Tan F (2012) In vitro study of the effects of Angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood–brain barrier (BBB). PLoS One 7(2):e32161
Intapan PM, Kittimongkolma S, Niwattayakul K, Sawanyawisuth K, Maleewong W (2008) Cerebrospinal fluid cytokine responses in human eosinophilic meningitis associated with angiostrongyliasis. J Neurolog Sci 267:17–21
Jitpimolmard S, Sawanyawisuth K, Morakote N, Vejjajiva A, Puntumetakul M, Sanchaisuriya K, Tassaneeyakul W, Tassaneeyakul W, Korwanich N (2007) Albendazole therapy for eosinophilic meningitis caused by Angiostrongylus cantonensis. Parasitol Res 100(6):1293–1296
Lai CH, Yen CM, Chin C, Chung HC, Kuo HC, Lin HH (2007) Eosinophilic meningitis caused by Angiostrongylus cantonensis after ingestion of raw frogs. AmJTrop Med Hyg 76:399–402
Lawson LJ, Perry VH, Dri P, Gordon S (1990) Heterogeneity in the distribution and morphology of microglia in the normal adult mouse brain. Neuroscience 39:151–170
Li ZY, Lv ZY, Wei J, Liao Q, Zheng HQ, Wu ZD (2012) Cloning and characterization of a novel gene encoding 16 kDa protein (Ac16) from Angiostrongylus cantonensis. Parasitol Res 110(6):2145–2153
Lv S, Zhang Y, Steinmann P, Zhou XN (2008) Emerging angiostrongyliasis in mainland China. Emerg Infect Dis 14:161–164
Njie EG, Boelen E, Stassen FR, Steinbusch HW, Borchelt DR, Streit WJ (2012) Ex vivo cultures of microglia from young and aged rodent brain reveal age-related changes in microglial function. Neurobiol Aging 33(1):195.e1–195.e12
Ouyang L, Wei J, Wu Z, Zeng X, Li Y, Jia Y, Ma Y, Zhan M, Lei W (2012) Differences of larval development and pathological changes in permissive and nonpermissive rodent hosts for Angiostrongylus cantonensis infection. Parasitol Res. doi:10.1007/s00436-012-2995-6
Sanderson CJ (1990) Eosinophil differentiation factor (interleukin-5). Immunol 49:231–256
Sawanyawisuth K, Takahashi K, Hoshuyama T, Sawanyawisuth K, Senthong V, Limpawattana P, Intapan PM, Wilson D, Tiamkao S, Jitpimolmard S, Chotmongkol V (2009) Clinical factors predictive of encephalitis caused by Angiostrongylus cantonensis. Am J Trop Med Hyg 81:698–701
Sugaya H, Aoki M, Abe T, Ishida K, Yoshimura K (1997) Cytokine responses in mice infected with Angiostrongylus cantonensis. Parasitol Res 83:10–15
Wallace GD, Rosen L (1966) Studies on eosinophilic meningitis. 2. Experimental infection of shrimp and crabs with Angiostrongylus cantonensis. Am J Epidemiol 84:120–131
Wang QP, Lai DH, Zhu XQ, Chen XG, Lun ZR (2008) Human angiostrongyliasis. Lancet Infect Dis 8:621–630
Wang J, Qi H, Diao Z, Zheng X, Li X, Ma S, Ji A, Yin C (2010) An outbreak of angiostrongyliasis cantonensis in Beijing. J Parasitol 96(2):377–381
Wang QP, Wu ZD, Wei J, Owen RL, Lun ZR (2012) Human Angiostrongylus cantonensis: an update. Eur J Clin Microbiol Infect Dis 31(4):389–395
Zhao H, Zhu C, Wei Y, Wang W (2007) The modified cultural method for isolation and purification for the microglia. Chin J Histochem Cytochem 1:115–120
Acknowledgments
This work was supported by the National Basic Research Program of China (2010CB530004) and National Nature Science Foundation of China (81271855,81261160324) to Z-D Wu. We would like to thank Zhao Hu (Tongji Medical College, Huazhong University of Science and Technology Department of Anatomy) and Yuan Guangqin (Experimental Teaching Center of Zhongshan School of Medicine, Sun Yat-sen University) for their assistance in guild of culture technology.
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Wei, J., Wu, F., Sun, X. et al. Differences in microglia activation between rats-derived cell and mice-derived cell after stimulating by soluble antigen of IV larva from Angiostrongylus cantonensis in vitro. Parasitol Res 112, 207–214 (2013). https://doi.org/10.1007/s00436-012-3127-z
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DOI: https://doi.org/10.1007/s00436-012-3127-z