Parasitology Research

, Volume 111, Issue 5, pp 1947–1952 | Cite as

The kinetics of oocyst shedding and sporulation in two immunologically distinct strains of Eimeria maxima, GS and M6

  • Riadh Al-Badri
  • John Robert BartaEmail author
Original Paper


The kinetics of oocyst shedding and sporulation of two immunologically distinct strains of Eimeria maxima (GS and M6) were compared. Both strains had a prepatent period of approximately 120 h followed by peak oocyst shedding at 144-150 h post inoculation. Mean total oocyst output determined for each strain demonstrated that the fecundity of the M6 strain (12.8 × 103 ± 1.95) of E. maxima was roughly twice that of the GS strain (6.9 × 103 ± 3.33) when inoculated at the rate of 1,000 infective oocysts per bird. The process of oocyst sporulation was followed by repetitive sampling of sporulating oocysts at 26 °C with aeration over a 138 hour period. Sporulation was divided into five morphologically distinguishable stages whose abundance peaked at the following times during sporulation: unsporulated oocysts at 0 h; sporoblast anlagen at 18 h; sporoblasts without sporocyst walls at 22 h; and sporocysts without mature sporozoites at 38 h. The time to 50 % sporulation of E. maxima oocysts observed in the present study was approximately 53 h for both strains and all viable oocysts had completed sporulation by 60 h. In the present study, the prepatent periods, duration of oocyst shedding, and the relative kinetics of sporulation of the GS and M6 strains of E. maxima were found to be virtually identical despite the immunological distinctiveness of these two parasite strains.


Coccidiosis Prepatent Period Eimeria Species Stieda Body Unsporulated Oocyst 
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The authors thank The Iraqi Ministry of Higher Education and Scientific Research for supporting RAB. The Natural Sciences and Engineering Research Council of Canada and the Ontario Ministry of Agriculture, Food and Rural Affairs are acknowledged for funding this research through grants to JRB. Julie Cobean is thanked for her technical support during this project.


  1. Allen PC, Jenkins MC, Miska KB (2005) Cross protection studies with Eimeria maxima strains. Parasitol Res 97:179–185PubMedCrossRefGoogle Scholar
  2. Barta JR, Coles BA, Schito ML, Fernando MA, Martin A, Danforth HD (1998) Analysis of infraspecific variation among five strains of Eimeria maxima from North America. Int J Parasitol 28:485–492PubMedCrossRefGoogle Scholar
  3. Beattie SA, Fernando MA, Barta JR (2001) A comparison of sporozoite transport after homologous and heterologous challenge in chickens immunized with the Guelph strain or the Florida strain of Eimeria maxima. Parasitol Res 87:116–121PubMedCrossRefGoogle Scholar
  4. Beesley JE, Latter VS (1982) The sporulation of Eimeria tenella as revealed by a novel preparative method. Z Parasitenkd 67:255–260PubMedCrossRefGoogle Scholar
  5. Canning EU, Anwar M (1968) Studies on meiotic division in coccidial and malarial parasites. J Protozool 15:290–298PubMedGoogle Scholar
  6. Dürr U, Heunert HH, Milthaler B (1971) Sporogony of Eimeria stiedai (Protozoa, Sporozoa). Acta Vet Acad Sci Hung 21:421–432PubMedGoogle Scholar
  7. Edgar SA (1954) Effect of temperature on the sporulation of oocysts of the protozoan, Eimeria tenella. T Am Microsc Soc 73:237–242CrossRefGoogle Scholar
  8. Edgar SA (1955) Sporulation of oocysts at specific temperatures and notes on the prepatent period of several species of avian coccidia. J Parasitol 41:214–216PubMedCrossRefGoogle Scholar
  9. Fergusson DJ, Birch-Anderson A, Hutchison WM, Siim JC (1978) Light and electron microscopy on the oocysts of Eimeria brunetti I. Development of the zygote and formation of the sporoblasts. Acta Pathol Microbiol Scand 86:1–11Google Scholar
  10. Hammond DM (1973) Life cycles and development of coccidia. In: Hammond DM, Long PL (eds) The Coccidia: eimeria, Isospora, Toxoplasma and related genera. University Park Press, Baltimore, pp 45–79Google Scholar
  11. Johnston WT, Shirley MW, Smith AL, Gravenor MB (2001) Modelling host cell availability and the crowding effect in Eimeria infections. Int J Parasitol 31:1070–1081PubMedCrossRefGoogle Scholar
  12. Kinnaird JH, Bumstead JM, Mann DJ, Ryan R, Shirley MW, Shiels BR, Tomley FM (2004) EtCRK2, a cyclin-dependent kinase gene expressed during the sexual and asexual phases of the Eimeria tenella life cycle. Int J Parasitol 34:683–692PubMedCrossRefGoogle Scholar
  13. Lillehoj HS, Trout JM (1993) Coccidia: a review of recent advances on immunity and vaccine development. Avian Pathol 22:3–31PubMedCrossRefGoogle Scholar
  14. Marquardt WC, Senger CM, Seghetti L (1960) The effect of physical and chemical agents on the oocysts of Eimeria zuernii (Protozoa, Coccidia). J Protozool 7:186–189Google Scholar
  15. Martin AG, Danforth HD, Barta JR, Fernando MA (1997) Analysis of immunological cross-protection and sensitivities to anticoccidial drugs among five geographical and temporal strains of Eimeria maxima. Int J Parasitol 27:527–533PubMedCrossRefGoogle Scholar
  16. McDonald V, Shirley MW, Bellatti MA (1986) Eimeria maxima: characteristics of attenuated lines obtained by selection for precocious development in the chicken. Exp Parasitol 61:192–200PubMedCrossRefGoogle Scholar
  17. Norton CC, Chard MJ (1983) The oocyst sporulation time of Eimeria species from the fowl. Parasitology 86:193–198PubMedCrossRefGoogle Scholar
  18. Ogedengbe JD, Hanner RH, Barta JR (2011) DNA barcoding identifies Eimeria species and contributes to the phylogenetics of coccidian parasites (Eimeriorina, Apicomplexa, Alveolata). Int J Parasitol 41:843–850PubMedCrossRefGoogle Scholar
  19. Peek HW, Landman WJ (2011) Coccidiosis in poultry: anticoccidial products, vaccines and other prevention strategies. Vet Q 31:143–161PubMedCrossRefGoogle Scholar
  20. Reid WM, Long PL (1979) A diagnostic chart for nine species of fowl coccidia. Univ Ga Coll Agric Res Rep 335:1–24Google Scholar
  21. Ryley JF, Meade R, Hazelhurst J, Robinson TE (1976) Methods in coccidiosis research: separation of oocysts from faeces. Parasitology 73:311–326PubMedCrossRefGoogle Scholar
  22. Shirley MW, Smith AL, Blake DP (2007) Challenges in the successful control of the avian coccidia. Vaccine 25:5540–5547PubMedCrossRefGoogle Scholar
  23. Tyzzer EE (1929) Coccidiosis in gallinaceous birds. Am J Hyg 10:269–383Google Scholar
  24. Waldenstedt L, Elwinger K, Lundén A, Thebo P, Uggla A (2001) Sporulation of Eimeria maxima oocysts in litter with different moisture contents. Poult Sci 80:1412–1415PubMedGoogle Scholar
  25. Williams RB (2001) Quantification of the crowding effect during infections with the seven Eimeria species of the domesticated fowl: its importance for experimental designs and the production of oocyst stocks. Int J Parasitol 31:1056–1069PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Department of Pathobiology, Ontario Veterinary CollegeUniversity of GuelphGuelphCanada

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