Comparative evaluation of five Beauveria isolates for housefly (Musca domestica L.) control and growth optimization of selected strain
- 355 Downloads
Pathogenic potential of five native Beauveria isolates was assessed against housefly adult and larvae in laboratory bioassays. Beauveria isolate Beauveria bassiana HQ917687 showed highest virulence with 72.3 and 100 % mortality of larvae and adults of Musca domestica, respectively. Other Beauveria isolates caused 36–52 % housefly larval mortality while the adult mortalities varied between 72 and 82 %. B. bassiana HQ917687 also showed the fastest killing activity with LT50 of 4 days (for larvae) and 3 days (for adults). This isolate showing highest virulence was selected for its growth optimization in terms of biomass and spore production using response surface methodology. The optimum value of temperature, yeast extract, and pH for maximum biomass and spore production was predicted as 27 °C, 5.00 g/l, and 6.75, respectively. Temperature was found to be the most critical factor influencing biomass and spore yield of the fungus and even nullified the effects of other factors at sufficiently higher value. The results obtained in this study depict the significance of appropriate strain selection and process parameter optimization in order to facilitate mass production of biocontrol agents.
KeywordsYeast Extract Response Surface Methodology Biomass Yield Entomopathogenic Fungus Spore Production
This work was supported by the Indian Council of Medical Research (IRIS_ID no. 2010–07860), India. CSIR fellowship to one of the authors (SM) is gratefully acknowledged. The authors acknowledge Mr. Peeyush Kumar (IIT Delhi, India) for his help in experimental work.
- Abbott WS (1925) A method for computing the effectiveness of an insecticide. J Econ Entomol 18:265–267Google Scholar
- Ferron P, Fargues J, Riba G (1991) Fungi as microbial insecticides against pests. In: Arora DK, Ajello L, Mukerjii KG (eds) Handbook of applied mycology, vol 2. Marcel Dekker, New York, pp 665–706Google Scholar
- Im DJ, Aguda RM, Rombach MC (1988) Effects of nutrients and pH on the growth and sporulation of four entomogenous hyphomycetes fungi (Deuteromycotina). Korean J Appl Entomol 27:41–46Google Scholar
- Kumar P, Mishra S, Malik A, Satya S (2012) Insecticidal evaluation of essential oils of Citrus sinensis L. (Myrtales: Myrtaceae) against housefly, Musca domestica L. (Diptera: Muscidae). Parasitol Res 110:1929–1936Google Scholar
- Mwamburi LA, Laing MD, Miller RM (2010) Laboratory screening of insecticidal activities of Beauveria bassiana and Paecilomyces lilacinus against larval and adult house fly (Musca domestica L.), Afr Entomol 18:38–46Google Scholar
- Pirali-Kheirabadi K, Haddadzadeh H, Razzaghi-Abyaneh M, Bokaie S, Zare R, Ghazavi M, Shams-Ghahfarokhi M (2007) Biological control of Rhipicephalus (Boophilus) annulatus by different strains of Metarhizium anisopliae, Beauveria bassiana and Lecanicillium psalliotae fungi. Parasitol Res 100:1297–1302PubMedCrossRefGoogle Scholar
- Senthamizhlselvan P, Alice J, Sujeetha RP, Jeyalakshmi C (2010) Growth, sporulation and biomass production of native entomopathogenic fungal isolates on a suitable medium. J Biopestic 3:466–469Google Scholar
- Siri A, Scorsetti AC, Dikgolz VE, Lopez Lastra CC (2005) Natural infection caused by the fungus Beauveria bassiana as a pathogen of Musca domestica in the neotropic. Biol Control 50:937–940Google Scholar
- SPSS (2008) Statistical product and service solution, system user's guide, version 22.214.171.124. Polar Engineering and Consulting, United State. http://www.winwrap.com/
- StatPlus (2007) Professional build 126.96.36.199. AnalystSoft, Inc., Kharkiv, UkraineGoogle Scholar