Giardia intestinalis: effects of Pulsatilla chinensis extracts on trophozoites
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Pulsatilla chinensis is a medicinal root plant that has been used to treat a wide range of disease conditions. Our study determined the antiprotozoal activity of various P. chinensis extracts and fractions against Giardia intestinalis including their effects on parasite growth, cell viability, adherence, and morphology. Ethyl acetate extracts (IC50 = 257.081 μg/ml) were the most active to inhibit the growth of G. intestinalis followed by aqueous extract (PWE), saponins, and n-butanol extract. The PWE and ethyl acetate extract inhibited G. intestinalis trophozoites adherence after 3 h of incubation and killed almost 50 % of the parasite population in a time-dependent manner. Changes in morphology, presence of precipitates in the cytoplasm, dissolved cytoplasm with large vacuole, break of flagella and ventral disk, membrane blebs, and intracellular and nuclear clearance of the treated trophozoites were observed by scanning and transmission electron microscopy. We demonstrated that P. chinensis induced these changes in G. intestinalis morphology and consequently has potential therapeutic use against giardiasis.
KeywordsSaponin Ethyl Acetate Extract Giardiasis IC50 Concentration Growth Inhibition Assay
This study was supported by Major Program of Preventive and Control for National Sever Infective Diseases (No. 2008ZX10004-001), National Natural Science Foundation of the People’s Republic of China (No. 31101804), and Key Discipline Construction Program of Anhui Science and Technology University (AKXK20101-2). We declare that the experiments comply with the current laws of China when they were performed.
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