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Cytotoxic effects of andiroba oil (Carapa guianensis) in reproductive system of Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) semi-engorged females

Parasitology Research volume 111, pages 1885–1894 (2012)Cite this article

Abstract

The present study performed an analysis about the effects of andiroba seed oil (Carapa guianensis) in the ovary of Rhipicephalus sanguineus semi-engorged females; once, there are few studies about the action of natural products on the reproductive system, a vital organ for the biological success of this animal group. The results showed that andiroba oil is a potent natural agent which causes significant structural changes in the oocytes, such as the emergence of large vacuolated cytoplasmic regions, reduction in the number of yolk granules, changes in the shape of the cells, as well as impairment of genetic material. In addition, the ovary epithelium showed severe morphological changes, such as extreme structural disorganization, with highly vacuolated cells and picnotic nuclei, forming an amorphous mass. This study showed also that oocytes (mainly in the initial stages of development) and the ovary epithelium of R. sanguineus females subjected to different concentrations of andiroba oil presented morphological changes which became more numerous and intense as the concentration of the product increased. Based on the results, it can be inferred that although the defense mechanisms are developed by oocytes to recover the cellular integrity (presence of autophagic vacuoles), these cells are not able to revert the damage caused by this product. Thus, it can be concluded that although the damages caused to the oocytes by andiroba oil are comparatively less severe than the ones caused by synthetic acaricides, this product can be considered a potent natural agent that reduce and/or prevent the reproduction of R. sanguineus females, with the advantage of not causing environmental impact such as synthetic chemical acaricides.

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Acknowledgments

The authors are grateful to Fundação de Amparo à Pesquisa do Estado de São Paulo (grant nos. 2011/07938-9, 2011/10427-6, 2010/51942-8, and 2011/06865-8) for the financial support.

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  1. UNESP—Univ. Estadual Paulista, São Paulo, Brazil

    Maria Cláudia Ramalho Vendramini, Maria Izabel Camargo-Mathias, Adriano Uemura de Faria, Gervásio Henrique Bechara, Patrícia Rosa de Oliveira & Gislaine Cristina Roma

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  1. Maria Cláudia Ramalho Vendramini
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  2. Maria Izabel Camargo-Mathias
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  3. Adriano Uemura de Faria
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  4. Gervásio Henrique Bechara
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Correspondence to Gislaine Cristina Roma.

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Vendramini, M.C.R., Camargo-Mathias, M.I., de Faria, A.U. et al. Cytotoxic effects of andiroba oil (Carapa guianensis) in reproductive system of Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) semi-engorged females. Parasitol Res 111, 1885–1894 (2012). https://doi.org/10.1007/s00436-012-3031-6

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Keywords

  • Yolk Granule
  • Ovary Epithelium
  • Synthetic Acaricide
  • Picnotic Nucleus
  • Severe Morphological Change