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Anthelminthic efficacy of banana crop residues on gastrointestinal nematodes of sheep: in vitro and in vivo tests

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Abstract

Resistance to anthelminthics is common due to intensive and incorrect use. In searching for alternatives, extracts of banana plant were evaluated for egg hatching inhibition and fecal egg count reduction of sheep nematodes. Aqueous extracts of the leaf, pseudostem, and heart of the banana plant cv. Prata anã were tested at concentrations of 0.31, 0.62, 1.25, 2.5, 5.0, and 10.0 mg ml−1 in egg hatching inhibition tests. For in vivo analysis, aqueous extracts were evaluated at dosages calculated according to the 10% lethal dose derived from acute toxicity testing in mice. Efficacy was evaluated at two time periods following oral administration. For the banana extracts at 2.5 mg ml−1, egg hatching was significantly fewer than the negative control, with an LC50 and LC90 of 0.19 and 0.84 mg ml−1, respectively. In vivo analysis for weeks 1 and 2 following a single treatment with aqueous leaf extract showed 33.1% and 32.5% anthelminthic efficacy, respectively. Further research on higher dosages with more frequent administration is needed to evaluate the potential for utilizing banana plant residues in gastrointestinal nematode control.

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Abbreviations

DM:

Dry matter

EHI:

Egg hatching inhibition

FEC:

Fecal egg count

GNIs:

Gastrointestinal nematodes

LC:

Lethal concentration

LD:

Lethal dose

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Acknowledgments

We thank the Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), Banco do Nordeste, and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) of Brazil for providing financial support. The authors are also grateful to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil), PRPq/UFMG, and to Associação Central dos Fruticultores do Norte de Minas (Abanorte).

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Authors and Affiliations

  1. Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, Av Universitária 1000, Bairro Universitário, Montes Claros, MG, 39400-006, Brazil

    Flávia Aparecida Nogueira, Lincoln Nunes Oliveira, Rayana Brito da Silva, Patrícia Silva Nery, Gercino Ferreira Virgínio Jr, Luciana Castro Geraseev & Eduardo Robson Duarte

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  1. Flávia Aparecida Nogueira
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  2. Lincoln Nunes Oliveira
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  3. Rayana Brito da Silva
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  4. Patrícia Silva Nery
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  5. Gercino Ferreira Virgínio Jr
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  6. Luciana Castro Geraseev
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  7. Eduardo Robson Duarte
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Correspondence to Eduardo Robson Duarte.

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Nogueira, F.A., Oliveira, L.N., da Silva, R.B. et al. Anthelminthic efficacy of banana crop residues on gastrointestinal nematodes of sheep: in vitro and in vivo tests. Parasitol Res 111, 317–323 (2012). https://doi.org/10.1007/s00436-012-2842-9

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  • DOI: https://doi.org/10.1007/s00436-012-2842-9

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