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Molecular test for vivax malaria with loop-mediated isothermal amplification method in central China

Parasitology Research volume 110, pages 2439–2444 (2012)Cite this article

Abstract

Vivax malaria has the highest incidence in central China. High-throughput and cost-effective testing methods are essential for vivax malaria diagnosis in this area. Loop-mediated isothermal amplification (LAMP) is an established nucleic acid amplification method, which provides a promising platform for the molecular detection of malaria parasites in development countries. This study was performed to compare the LAMP method, the nested PCR-based method, and microscopic examinations in diagnosing vivax malaria. LAMP detected vivax malaria parasites in 160 of 164 microscopically positive samples (sensitivity, 97.6%), whereas nested PCR detected Plasmodium vivax in 162 of 164 samples (sensitivity, 98.8%). No false-positive results were obtained by LAMP or nested PCR among fever-positive and healthy samples. The sensitivities and specificities of the two molecular tests were consistently high. In addition, the reproducibility of the LAMP assays using water bath, which reduced the cost substantially. LAMP method is an accurate, rapid, simple, and cost-effective method that may be useful for diagnosis in field diagnoses instead of nested PCR. This method is feasible to diagnose vivax malaria parasite in endemic areas of central China.

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Acknowledgments

This work was supported by a National Research Foundation of Korea Grant funded by the Korean Government (2009-0075103) and the Mid-Career Researcher Program through NRF grant funded by the MEST (No. 2011-0016401) of Korea and the Asia Pacific Malaria Elimination Network Establishment Support Programme (No. 107-02), the National S & T Major Programme (No. 2008ZX10004-011), and the National Natural Science Foundation of China (No. 30872214).

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Affiliations

  1. Department of Medical Environmental Biology and Tropical Medicine and Institute of Medical Science, Kangwon National University School of Medicine, Hyoja2-dong, Chuncheon, 200-701, Gangwon-do, Republic of Korea

    Feng Lu & Eun-Taek Han

  2. Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Jiangsu Institute of Parasitic Diseases, Wuxi, 214064, People’s Republic of China

    Feng Lu, Qi Gao, Huayun Zhou, Jun Cao & Weimin Wang

  3. Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Republic of Korea

    Chae Seung Lim

  4. Department of Obstetrics and Gynecology and Institute of Medical Science, Kangwon National University School of Medicine, Hyoja2-dong, Chuncheon, 200-701, Gangwon-do, Republic of Korea

    SungHun Na

  5. Cell-free Science and Technology Research Center, Ehime University, Matsuyama, Ehime, 790-8577, Japan

    Takafumi Tsuboi

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  1. Feng Lu
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  2. Qi Gao
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  3. Huayun Zhou
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  4. Jun Cao
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  5. Weimin Wang
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  6. Chae Seung Lim
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  7. SungHun Na
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  8. Takafumi Tsuboi
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  9. Eun-Taek Han
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Correspondence to Eun-Taek Han.

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Feng Lu and Qi Gao contributed equally to this work.

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Lu, F., Gao, Q., Zhou, H. et al. Molecular test for vivax malaria with loop-mediated isothermal amplification method in central China. Parasitol Res 110, 2439–2444 (2012). https://doi.org/10.1007/s00436-011-2783-8

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  • DOI: https://doi.org/10.1007/s00436-011-2783-8

Keywords

  • Malaria
  • Vivax Malaria
  • Lamp Assay
  • Babesia
  • Lamp Reaction