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Differences in the in vitro susceptibility to resveratrol and other chemical compounds among several Philasterides dicentrarchi isolates from turbot

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Abstract

Philasterides dicentrarchi is a histophagous scuticociliate that causes important losses in aquaculture. Several strains that differ in morphological, genetic and serological characteristics and virulence have been isolated from outbreaks of turbot scuticociliatosis. In the present study, seven isolates of the ciliate were exposed in vitro to formalin, hydrogen peroxide and resveratrol (a phytoalexin produced by plants) in order to evaluate the susceptibility of the isolates to the different compounds. The LD50 values for the three compounds tested varied widely among the isolates. The LD100 values were similar among isolates for formalin (25–30 ppm) and resveratrol (60–70 ppm) but were very different for hydrogen peroxide (25–>80 ppm). The results indicate that there are many physiological differences among isolates and even among specimens of the same isolates, which must be taken into account in designing control programmes. The naturally occurring resveratrol may be a good alternative to other compounds for reducing the amounts of viable ciliates in water.

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Acknowledgments

This work was financially supported by grants 08MMA007237PR, 08MMA018E and 08MMA025E from the Xunta de Galicia; AGL2006-12072-CO2-01/ACU, AGL2008-03866/ACU, AGL2010-21219/ACU and AGL2011-29927-CO2-01 from the Comisión Interministerial de Ciencia y Tecnología and INCITE07/PX1203039ES from Aquagenomics project funded through the Consolider-Ingenio 2010 programme by the Spanish Ministerio de Eduación y Ciencia.

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Authors and Affiliations

  1. Departamento de Biología Celular y Ecología, Facultad de Biología, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain

    B. Budiño & J. Lamas

  2. Pyrenean Institute of Ecology, CSIC, Jaca, Spain

    M. P. Pata

  3. Laboratorio de Parasitología, Instituto de Investigación y Análisis Alimentarios, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain

    J. Leiro

  4. Departamento de Biología Celular y Ecología, Edificio CIBUS, Rúa Lope Gómez de Marzoa, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain

    J. Lamas

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  1. B. Budiño
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  2. M. P. Pata
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  3. J. Leiro
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  4. J. Lamas
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Correspondence to J. Lamas.

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Budiño, B., Pata, M.P., Leiro, J. et al. Differences in the in vitro susceptibility to resveratrol and other chemical compounds among several Philasterides dicentrarchi isolates from turbot. Parasitol Res 110, 1573–1578 (2012). https://doi.org/10.1007/s00436-011-2664-1

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  • DOI: https://doi.org/10.1007/s00436-011-2664-1

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