Abstract
Prevalence of Hepatozoon caimani has been reported in 76% of caimans Caiman yacare from the Pantanal region. Culex (Melanoconion) spp. mosquitoes were recently identified as natural vectors of this parasite. However, culicids are not typically eaten by crocodilians, suggesting that the main transmission route is through ingestion of insectivorous vertebrates, such as anurans. The susceptibility of wild frogs Leptodactylus chaquensis, Leptodactylus podicipinus and Scinax nasicus to infection by H. caimani was verified. Wild-caught anurans were force fed with sporulated oocysts from laboratory-bred Culex (Melanoconion) mosquitoes. Frogs were killed 30 days postinfection, and their internal organs were fed to caimans C. yacare and Caiman latirostris. Cystozoites were identified in fresh liver impression smears of L. chaquensis. C. yacare fed on anuran organ presented gametocytes in peripheral blood circulation between 74 and 80 days postinoculation (dpi). Gametocytes were also verified in C. latirostris fed on the internal organs of L. podicipinus and S. nasicus between 60–70 and 69–75 dpi, respectively. Since frogs used in experiment are sympatric with C. yacare and C. latirostris and may occur in the diet of these caimans, the results suggest these amphibians are paratenic hosts in the natural transmission cycle of H. caimani in Pantanal.
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References
Ávila RW, Ferreira VL (2004) Riqueza e densidade de vocalizações de anuros (Amphibia) em uma área urbana de Corumbá, Mato Grosso do Sul, Brasil. Braz J Biol 21:887–892
Borteiro C, Gutiérrez F, Tedros M, Kolenc F (2009) Food habits of the broad-snouted caiman (Caiman latirostris: Crocodylia, Alligatoridae) in northwestern Uruguay. Stud Neotrop Fauna Environ 44:31–36
da Silveira R, Magnusson WE (1999) Diets of splectacled and black caiman in the Anavilhanas Archipelago, Central Amazonia, Brazil. J Herpetol 33:181–192
Desser SS (1990) Tissue “cysts” of Hepatozoon griseisciuri in the grey squirrel, Sciurus carolinensis: the significance of these cysts in species of Hepatozoon. J Parasitol 76:257–259
Gordo M, Campos Z (2003) Listagem dos Anuros da Estação Ecológica Nhumirim e arredores, Pantanal Sul. EMBRAPA-Pantanal, Corumbá
Johnson EM, Allen KE, Breshears MA, Pancieira RJ, Little SE, Ewing SA (2008) Experimental transmission of Hepatozoon americanum to rodents. Vet Parasitol 151:164–169
Johnson EM, Allen KE, Pancieira RJ, Ewing SA, Little SE (2009) Experimental transmission of Hepatozoon americanum to New Zealand white rabbits (Oryctolagus cuniculus) and infectivity of cystozoites for a dog. Vet Parasitol 164:162–166
Köppen W (1931) Climatologia. Fondo de Cultura Económica, Buenos Aires
Lainson R, Paperna I, Naiff RD (2003) Development of Hepatozoon caimani (Carini, 1909) Pessôa, De Biasi & De Souza, 1972 in the caiman Caiman c. crocodilus, the frog Rana catesbeiana and the mosquito Culex fatigans. Mem I Oswaldo Cruz 98:103–113
Landau I, Michel JC, Chabaud AG (1972) Cycle biologique d’Hepatozoon domerguei; discussion sur les caracteres fondamentaux d’un cycle de Coccidie. Z Parasitenk 38:250–270
Magnusson WE, Silva EV, Lima AP (1987) Diets of amazonian crocodilians. J Herpetol 21:85–95
Paperna I, Lainson R (2004) Hepatozoon cf. terzii (Sambon and Seligman, 1907) infection in the snake Boa constrictor constrictor from north Brazil: transmission to the mosquito Culex quinquefasciatus and the lizard Tropidurus torquatus. Parasite 11:175–181
Prado CPA, Haddad C (2003) Testes size in leptodactylid frogs and occurrence of multimale spawning in the genus Leptodactylus in Brazil. J Herpetol 37:354–362
Santos AS, Stoll MN, Pinheiro MS, Campos Z, Magnusson WE, Mourão G (1996) Diets of Caiman crocodilus yacare from different habitats in Brazilian Pantanal. J Herpetol 6:111–117
Sloboda M, Kamler M, Bulantová J, Votýpka J, Modrý D (2008) Rodents as intermediate hosts of Hepatozoon ayorgbor (Apicomplexa: Adeleina: Hepatozoidae) from the African ball python, Python regius? Folia Parasitol 55:13–16
Smith TG, Desser SS, Martin DS (1994) The development of Hepatozoon sipedon n. sp. (Apicomplexa: Adeleina: Hepatozoidae) in its natural host, the Northern water snake (Nerodia sipedon sipedon), in the culicine vectors Culex pipiens and C. territans, and in an intermediate host, the Northern leopard frog (Rana pipiens). Parasitol Res 80:559–568
Uetanabaro M, Prado CPA, Rodrigues DJ, Gordo M, Campos Z (2008) Field guide to the anurans of the south Pantanal and surrounding cerrados. Universidade Federal do Mato Grosso do Sul, Brazil
Viana LA, Marques EJ (2005) Haemogregarine parasites (Apicomplexa: Hepatozoidae) in Caiman crocodilus yacare (Crocodilia: Alligatoridae) from Pantanal, Corumbá, MS, Brazil. Rev Bras Parasitol Vet 14:173–175
Viana LA, Paiva F, Coutinho ME, Lourenço-de-Oliveira R (2010a) Hepatozoon caimani (Apicomplexa: Hepatozoidae) in wild caiman, Caiman yacare, from the Pantanal region, Brazil. J Parasitol 96:83–88
Viana LA, Soares P, Paiva F, Lourenço-de-Oliveira R (2010b) Caiman biting mosquitoes and the natural vector of Hepatozoon caimani (Apicomplexa: Hepatozoidae). J Med Entomol 47:670–676
Wallace KM, Leslie AJ (2008) Diet if the nile crocodile (Crocodylus niloticus) in Okavango Delta, Botswana. J Herpetol 42:361–368
Acknowledgments
The authors acknowledge the financial support received for this study from the Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT). The authors are grateful to Gerson Zahdi of the Cacimba de Pedra farm for kindly providing the young caimans.
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Viana, L.A., Soares, P., Silva, J.E. et al. Anurans as paratenic hosts in the transmission of Hepatozoon caimani to caimans Caiman yacare and Caiman latirostris . Parasitol Res 110, 883–886 (2012). https://doi.org/10.1007/s00436-011-2570-6
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DOI: https://doi.org/10.1007/s00436-011-2570-6