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Evaluation of time toxicity, residual effect, and growth-inhibiting property of Carapa guianensis and Copaifera sp. in Aedes aegypti

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Abstract

Oils of Carapa guianensis and Copaifera spp. are well-known in the Amazonian region as natural insect repellents, and studies have reported their efficiency as larvicide against some mosquito species. However, toxicity persistence and effect on mosquito development have not yet been evaluated. Thus, the objective of this study was to evaluate the initial time of larvicidal activity, residual effect, and the effect of very low concentrations of these oils on Aedes aegypti. Different concentrations of the oils were used to evaluate the initial time of larval mortality and residual effect, as well as, the development of larvae, pupae, and adults. Results demonstrated that the lethal effect started mainly between the first 2 and 3 h of larvae exposure to oils, when using concentrations which ranged from 500 mg/L of C. guianensis and 90 mg/L of Copaifera sp. The toxic effect remained with total efficiency (100% mortality) until the sixth day for Copaifera sp. and 12th day for C. guianensis. When using sublethal dosages (ranging from 140 mg/L of C. guianensis to 26 mg/L of Copaifera sp.) mortality was observed after the larval molt. Also, imperfection of pupae and adult development and unsuccessful emergence of adults were observed. A product of botanical origin that could break the development of immature stage of mosquitoes and inhibit the emergence of adults should be essential in vector control. Thus, our results provide new information for a better understanding in using C. guianensis and Copaifera sp. oils with a potential to be used as a natural insecticide.

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Acknowledgments

The authors acknowledge the support from the National Council of Research and Development (CNPq) which provided funds for the project process 410579/2006-8 and scholarship.

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Authors and Affiliations

  1. Setor de Ciências Biológicas, Departamento de Zoologia, Programa de Pós-graduação em Entomologia, Universidade Federal do Paraná, Caixa Postal 19020, CEP 81531-980, Curitiba, Paraná, Brazil

    Josiane S. Prophiro, Mario A. Navarro da Silva & Jonny E. Duque-Luna

  2. Grupo de Pesquisa em Imunoparasitologia–IMPAR, Universidade do Sul de Santa Catarina, Av. José Acácio Moreira 787, Dehon, 88704-900, Tubarão, Santa Catarina, Brazil

    Josiane S. Prophiro

  3. Grupo de Pesquisa em Tecnologia Farmacêutica–TECFARMA, Universidade do Sul de Santa Catarina, Av. José Acácio Moreira 787, Dehon, 88704-900, Tubarão, Santa Catarina, Brazil

    Luiz A. Kanis

  4. Grupo de Pesquisa em Imunoparasitologia–IMPAR, Universidade do Sul de Santa Catarina, Av. José Acácio Moreira 787, Dehon, 88704-900, Tubarão, Santa Catarina, Brazil

    Bruna M. da Silva

  5. ICBS, Departamento de Microbiologia, Parasitologia e Imunologia, Rua Sarmento Leite, Universidade Federal do Rio Grande do Sul, 500, sala 206, CEP 90050-170, Porto Alegre, Rio Grande do Sul, Brazil

    Onilda S. da Silva

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  1. Josiane S. Prophiro
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  2. Mario A. Navarro da Silva
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  3. Luiz A. Kanis
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  4. Bruna M. da Silva
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  5. Jonny E. Duque-Luna
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  6. Onilda S. da Silva
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Correspondence to Josiane S. Prophiro, Mario A. Navarro da Silva or Onilda S. da Silva.

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Prophiro, J.S., da Silva, M.A.N., Kanis, L.A. et al. Evaluation of time toxicity, residual effect, and growth-inhibiting property of Carapa guianensis and Copaifera sp. in Aedes aegypti . Parasitol Res 110, 713–719 (2012). https://doi.org/10.1007/s00436-011-2547-5

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  • DOI: https://doi.org/10.1007/s00436-011-2547-5

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