Skip to main content
SpringerLink
Log in

Biological control of Fasciola hepatica eggs with the Pochonia chlamydosporia fungus after passing through the cattle gastrointestinal tract

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

Fasciolosis is a disease caused by Fasciola hepatica responsible for causing significant losses in livestock. This study aimed to evaluate the Pochonia chlamydosporia fungus (isolate VC1) on F. hepatica eggs after passing through the cattle gastrointestinal tract. For this evaluation, 1 g pellet was given in sodium alginate matrix per kilogram live weight containing 25% of fungal mycelium from isolate VC1 per animal. Twelve animals were used, six treated and six untreated (control). Some stool samples were collected from the groups of treated and control animals, at the times of 12, 18, 24, 48, 72, and 96 h after the pellets' administration. Then, from each stool sample of treated and control groups, 2 g was placed in a Petri dish of 9 cm in diameter, containing 2% water–agar and 1,000 eggs of F. hepatica. It was observed that the fungus was effective in preying upon the eggs in the samples recovered at all of the schedules starting at 12 h. Furthermore, differences were observed (p < 0.01) in the destruction of eggs in the Petri dishes in the treated group compared with the control group. The ovicidal effect was observed after 7 days of interaction. The ovicidal P. chlamydosporia fungus was effective in destroying F. hepatica eggs; therefore, it is suggested that this fungus could be employed as agent for the control of helminth eggs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Araújo JV, Santos MA, Ferraz S (1995) Efeito ovicida de fungos nematófagos sobre ovos embrionados de Toxocara canis. Arq Bras Med Vet Zoot 47:37–42

    Google Scholar 

  • Araujo JM, Araújo JM, Braga FR, Carvalho RO, Ferreira SR (2009) Activity of the nematophagous fungi Pochonia chlamydosporia, Duddingtonia flagrans and Monacrosporium thaumasium on egg capsules of Dipylidium caninum. Vet Parasitol 166:6–89

    Article  Google Scholar 

  • Ayres M, Ayres JRM, Ayres DL, Santos AS (2007) Aplicações estatísticas nas áreas de ciências biomédicas, 5th edn. Belém Pará, Brasil, 280 pp

    Google Scholar 

  • Braga FR, Araújo JV, Campos AK, Araújo JM, Carvalho RO, Silva AR, Tavela AO (2008) In vitro evaluation of the action of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense and Pochonia chlamydosporia on Fasciola hepatica eggs. World J Microbiol Biotechnol 24:1559–1564

    Article  Google Scholar 

  • Braga FR, Araújo JV, Silva AR, Carvalho RO, Araújo JM, Ferreira SR, Carvalho GR (2010) Viability of the nematophagous fungus Pochonia chlamydosporia after passage through the gastrointestinal tract of horses. Vet Parasitol 168:264–268

    Article  PubMed  Google Scholar 

  • Braga FR, Silva AR, Carvalho RO, Araújo JV, Pinto PSA (2011) Ovicidal activity of different concentrations of Pochonia chlamydosporia chlamydospores on Taenia taeniaeformis eggs. J Helmintol 85:7–11

    Article  CAS  Google Scholar 

  • Dalton JP (1999) Fasciolosis, 1st edn. University Press, Cambridge

    Google Scholar 

  • De S, Sanyal PK, Sarkar AK, Patel NK, Pal S, Mandal SC (2008) Screening for Indian isolates of egg-parasitic fungi for use in biological control of fascioliasis and amphistomiasis in ruminant livestock. J Helminthol 82:271–277

    Article  PubMed  CAS  Google Scholar 

  • Dias AS, Araújo JV, Campos AK, Braga FR, Fonseca TA (2007) Application of a formulation of the nematophagous fungus Duddingtonia flagrans in the control of cattle gastrointestinal nematodioses. World J Microbiol Biotechnol 38:1245–1252

    Article  Google Scholar 

  • Echevarria F (2004) Fasciolose. Rev Bras Parasitol Vet 13:100–103

    Google Scholar 

  • Gams W, Zare R (2001) A revision of Verticillium sect. Prostrata. III. Generic classification. Nova Hedwigia 73:329–337

    Google Scholar 

  • García U, Bulnes C, Melchor G, Veja E, Ileana M, De Oca NM, Hidalgo L, Marrero E (2004) Safety of Pochonia chlamydosporia var catenulata in acute oral and dermal toxicity/pathogenicity evaluations in rats and rabbits. Vet Hum Toxicol 46:248–250

    PubMed  Google Scholar 

  • Kerry BR, Hidalgo L (2004) Application of Pochonia chlamydosporia in the integrated control of root-knot nematode on organically grown vegetable crops in Cuba. IOBC/WPRS Bulletin 27:123–126

    Google Scholar 

  • Larsen M (1999) Biological control of helminths. Int J Parasitol 29:139–146

    Article  PubMed  CAS  Google Scholar 

  • Lima WS, Soares LRM, Barçante TA, Guimaraes MP, Barçante JMP (2009) Occurrence of Fasciola hepatica (Linnaeus, 1758) infection in Brazilian cattle of Minas Gerais, Brazil. Rev Bras Parasitol Vet 18(2):27–30

    PubMed  Google Scholar 

  • Lysek H, Fassatiová O, Pineda NC, Hernández NL (1982) Ovicidal fungi in soils of Cuba. Folia Parasitol 29:265–270

    PubMed  CAS  Google Scholar 

  • Pile E, Santos JAA, Pastorello T, Vasconcellos M (2001) Fasciola hepatica em búfalos (Bubalus bubalis) no município de Maricá, Rio de Janeiro, Brasil. Braz J Vet Res An Sci 38(1):42–43

    Google Scholar 

  • Ross JG, Dow C, Todd JR (1997) A study of Fasciola hepatica infections in sheep. Vet Rec 80:543–6

    Article  Google Scholar 

  • Singh RK, Sanyal PK, Patel NK, Sarkar AK, Santra AK, Pal S, Mandal SC (2010) Fungus–benzimidazole interactions: a prerequisite to deploying egg-parasitic fungi Paecilomyces lilacinus and Verticillium chlamydosporium as biocontrol agents against fascioliasis and amphistomiasis in ruminant livestock. J Helmintol 84:123–131

    Article  CAS  Google Scholar 

  • Stirling GR (1991) Biological control of plant parasitic nematodes. CAB International, Wallingford, 282p

    Google Scholar 

  • Tavela AO, Araújo JV, Braga FR, Silva AR, Carvalho RO, Araujo JM, Ferreira SR, Carvalho GR (2011) Biological control of cyathostomin (Nematoda: Cyathostominae) with nematophagous fungus Monacrosporium thaumasium in tropical southeastern Brazil. Vet Parasitol 175:92–96

    Article  Google Scholar 

  • Terrill TH, Larsen M, Samples O, Husted S, Miller JE, Kaplan RM, Gelaye S (2004) Capability of the nematode-trapping fungus Duddingtonia flagrans to reduce infective larvae of gastrointestinal nematodes in goat feces in the southeastern United States: dose titration and dose time interval studies. Vet Parasitol 120:285–296

    Article  PubMed  CAS  Google Scholar 

  • Waller PJ, Larsen M, Faedo M, Henessy DR (1994) The potential of nematophagous fungi to control the free-living stages of nematodes parasites of sheep: in vitro and in vivo studies. Vet Parasitol 51:289–299

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

I want to thank CNPq, Fapemig, and Capes by financial support.

Author information

Authors and Affiliations

  1. Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil

    Anderson S. Dias, Jackson V. Araújo, Fábio R. Braga, Juliana M. Araujo & Fernanda M. Fernandes

  2. IESES/FACASTELO, Castelo, Espirito Santo, CEP 29360-000, Brazil

    Anderson S. Dias, André C. Puppin, Rafael F. Ramos, Raul M. Bertonceli, Renata G. da Silva & Wilber R. Perboni

Authors
  1. Anderson S. Dias
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jackson V. Araújo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fábio R. Braga
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Juliana M. Araujo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. André C. Puppin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fernanda M. Fernandes
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Rafael F. Ramos
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Raul M. Bertonceli
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Renata G. da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Wilber R. Perboni
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anderson S. Dias.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dias, A.S., Araújo, J.V., Braga, F.R. et al. Biological control of Fasciola hepatica eggs with the Pochonia chlamydosporia fungus after passing through the cattle gastrointestinal tract. Parasitol Res 110, 663–667 (2012). https://doi.org/10.1007/s00436-011-2538-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-011-2538-6

Keywords

Search

Navigation