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Vaccination of buffaloes with Fasciola gigantica recombinant glutathione S-transferase and fatty acid binding protein

Parasitology Research volume 110, pages 419–426 (2012)Cite this article

Abstract

Fasciola gigantica, causative agent of tropical fasciolosis, inflicts substantial economic losses on the livestock industry, affecting severely buffalo productivity in the tropical countries. Very few vaccination trials with different target antigens against F. gigantica infection have been conducted in this host. Present study describes a vaccination trial in buffaloes with F. gigantica recombinant glutathione S-transferase and fatty acid binding protein. The two recombinant proteins were expressed in Escherichia coli and evaluated for their immunoprophylactic potential in buffalo calves, using montanide 70 M-VG, a mineral oil-based adjuvant, for delivering the antigens. Buffalo calves were distributed in three groups, with group I, II and III calves immunized with recombinant glutathione S-transferase, fatty acid binding protein and a cocktail of these two antigens, respectively. Immunization of the calves evoked a mixed IgG1 and IgG2 antibody response. Present vaccination trial in these animals achieved a maximum protection level of 35%, when the two antigens were used in combination. Eosinophils were measured in both immunized and non-immunized challenge control animals, which showed a steady increase in their count in response to immunization with both the antigens and infection with F. gigantica, respectively.

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Acknowledgements

The authors are thankful to the Department of Biotechnology, Government of India, New Delhi for providing grants to this research project. We are also thankful to the Director, Indian Veterinary Research Institute, Izatnagar for providing necessary facilities for completion of this reserach work.

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  1. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243122, India

    Niranjan Kumar, Varghese Anju, Nagar Gaurav, Dinesh Chandra, S. Samanta, S. C. Gupta, J. Adeppa & O. K. Raina

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  1. Niranjan Kumar
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  2. Varghese Anju
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  3. Nagar Gaurav
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  4. Dinesh Chandra
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  5. S. Samanta
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  6. S. C. Gupta
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  7. J. Adeppa
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  8. O. K. Raina
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Correspondence to O. K. Raina.

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Kumar, N., Anju, V., Gaurav, N. et al. Vaccination of buffaloes with Fasciola gigantica recombinant glutathione S-transferase and fatty acid binding protein. Parasitol Res 110, 419–426 (2012). https://doi.org/10.1007/s00436-011-2507-0

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  • DOI: https://doi.org/10.1007/s00436-011-2507-0

Keywords

  • Fatty Acid Binding Protein
  • Eosinophil Count
  • Worm Burden
  • Vaccination Trial
  • Buffalo Calf