Abstract
Two novel genes, SJCWL05 and SJCWL06, were harvested from screening of Schistosoma japonicum (S. japonicum) cercaria cDNA library by using pig sera vaccinated (VPS) with S. japonicum immature egg ws-vaccine (S. japonicum iEw). Prokaryotic recombinant plasmids pGEX-4T-1/SJCWL05 and pGEX-4T-1/SJCWL06 were constructed to analyze their immunogenicity, which was confirmed by SDS-PAGE and Western blotting. Two eukaryotic recombinant plasmids, pcDNA3/SJCWL05 and pcDNA3/SJCWL06, were constructed, and their ability to protect mice against challenge of S. japonicum was evaluated. All mice vaccinated with pcDNA3/SJCWL05 or pcDNA3/SJCWL06 developed ELISA-specific anti-S. japonicum SIEA (S. japonicum soluble immature egg antigens) antibody. Immunoprotection experiments showed that worms and liver eggs reduced 34.64% and 39.14% in the pcDNA3/SJCWL05 group and those reduced 27.17% and 27.95% in the pcDNA3/SJCWL06 group, respectively. The reduction rates of intestine and uterine eggs in female worms of both groups reached 39.45% and 38.5% as well as 30.02% and 28.7%, respectively. Results of our study suggest that novel genes, SJCWL05 and SJCWL06, are potential vaccine candidates against schistosomiasis japonica.
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Acknowledgments
The authors would like to thank Professor Y.Q. Lu for critically reviewing the manuscript. This work was supported by Grants from the Key Basic Research and Development Program of China (973) (grant number 2007CB513108); the National Scientific and Technological Support Projects (grant number 2009BAI78B05); Key Laboratory of Immune and Control Schistosomiasis & Key Subject Development Special Program of Hunan Province (grant number 2008-985-2).
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Tian, Z., Wang, Sp., Zeng, Sh. et al. Characterization and vaccination of two novel Schistosoma japonicum genes screened from a cercaria cDNA library. Parasitol Res 110, 403–409 (2012). https://doi.org/10.1007/s00436-011-2505-2
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DOI: https://doi.org/10.1007/s00436-011-2505-2