Abstract
Serum and blood samples from 36 game animals, shot during the hunting seasons 2007–2009, were collected and analyzed for the presence of Trypanosoma spp. by three methods: isolation, polymerase chain reaction (PCR), and serology. Only fissiped animals were included, four different ruminants and wild boar. Trypanosomes could be isolated from two of the animals, and eight had detectable parasite DNA. Seven animals had high titers of anti-trypanosoma IgG antibodies. The two isolated strains, one from roe dear and one from European elk, were determined to Trypanosoma theileri by partial DNA sequencing of the 18S ribosomal gene. In the seven boars, no Trypanosoma were detected, but four out of seven strongly positive serological samples came from this group. This is the first study in Scandinavia on the presence of Trypanosoma in game animals. The results indicate that trypanosomiasis is frequently occurring among Swedish game animals.
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References
Antoine-Moussiaux N, Büscher P, Desmecht D (2009) Host-parasite interactions in trypanosomiasis: on the way to an antidisease strategy. Infect Immun 77:1276–1284
Botero A, Ortiz S, Muñoz S, Triana O, Solari A (2010) Differentiation of Trypanosoma cruzi and Trypanosoma rangeli of Colombia using minicircle hybridization tests. Diagn Microbiol Infect Dis 68:265–270
Burgdorfer W, Schmidt ML, Hoogstraal H (1973) Detection of Trypanosoma theileri in Ethiopian cattle ticks. Acta Trop 30:340–346
Desquesnes M, Dávila AM (2002) Applications of PCR-based tools for detection and identification of animal trypanosomes: a review and perspectives. Vet Parasitol 109:213–231
Dirie MF, Bornstein S, Wallbanks KR, Molyneux DH, Steen M (1990) Comparative studies on Megatrypanum trypanosomes from cervids. Trop Med Parasitol 41:198–202
Geysen D, Delespaux V, Geerts S (2003) PCR-RFLP using Ssu-rDNA amplification as an easy method for species-specific diagnosis of Trypanosoma species in cattle. Vet Parasitol 110:171–180
Jelinek T, Bisoffi Z, Bonazzi L, van Thiel P, Bronner U, de Frey A, Gundersen SG, McWhinney P, Ripamonti D (2002) European Network on Imported Infectious Disease Surveillance. Cluster of African trypanosomiasis in travelers to Tanzanian national parks. Emerg Infect Dis 8:634–635
Kennedy PG (2008) The continuing problem of human African trypanosomiasis (sleeping sickness). Ann Neurol 64:116–126
Kingston N, Thorne ET, Thomas GM, McHolland L, Trueblood MS (1981) Further studies on trypanosomes in game animals in Wyoming II. J Wildl Dis 17:539–546
Magona JW, Walubengo J, Olaho-Mukani W, Jonsson NN, Eisler MC (2009) Diagnostic value of rectal temperature of African cattle of variable coat colour infected with trypanosomes and tick-borne infections. Vet Parasitol 160:301–305
Morton JK, Kingston N (1976) Further studies on trypanosomes in game animals in Wyoming. J Wildl Dis 12:233–236
Rassi A Jr, Rassi A, Marin-Neto JÁ (2010) Chagas disease. Lancet 375:1388–1402
Verloo D, Brandt J, Van Meirvenne N, Büscher P (2000) Comparative in vitro isolation of Trypanosoma theileri from cattle in Belgium. Vet Parasitol 89:129–132
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Neumüller, M., Nilsson, K. & Påhlson, C. Trypanosoma spp. in Swedish game animals. Parasitol Res 110, 135–139 (2012). https://doi.org/10.1007/s00436-011-2462-9
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DOI: https://doi.org/10.1007/s00436-011-2462-9