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Redescription and molecular analysis of Myxobolus shantungensis Hu, 1965 (Myxozoa: Myxosporea) infecting common carp Cyprinus carpio haematopterus

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Myxobolus shantungensis Hu, 1965 infects gill arches of common carp Cyprinus carpio haematopterus (Temminck and Schlegel), causing serious pathological effects on host fish. An inadequate original description and absence of molecular data make accurate early diagnosis challenging. To augment the original description, M. shantungensis is redescribed here using morphological and molecular biological methods. Mature spores of M. shantungensis were ellipsoidal or apple shaped in frontal view and lemon shaped in lateral view, averaging 8.2 ± 0.3 μm (8.0–9.0 μm) × 10.1 ± 0.5 μm (9.2–11.1 μm) × 6.9 ± 0.3 μm (6.0–7.4 μm). Some spores had three to four “V”-shaped valve edge markings on the posterior of the spore. The two equal polar capsules were oval, measuring 4.3 ± 0.3 μm (3.8–5.0 μm) × 3.2 ± 0.2 μm (2.8–3.5 μm), situated at the anterior extremity of the spore. Polar filaments coiled with six to seven turns. Scanning electron microscopy revealed that the surface of mature spores of M. shantungensis was generally pitted with a number of irregular ridges in shape. M. shantungensis is also characterized on the molecular level using the sequence of the small subunit ribosomal RNA gene. A BLAST search revealed that this sequence did not match any available sequences in GenBank.

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  • Adriano EA, Arana S, Alves AL, Silva MR, Ceccarelli PS, Henrique-Silva F, Maia AA (2009) Myxobolus cordeiroi n. sp., a parasite of Zungaro jahu (Siluriformes: Pimelodiade) from Brazilian Pantanal: morphology, phylogeny and histopathology. Vet Parasitol 162:221–229

    Article  PubMed  CAS  Google Scholar 

  • Bylund G, Fagerholm H, Calenius G, Wikgren B, Wikstrom M (1980) Parasites of fish in Finland. I. Introduction. Acta Academiae Aboensis 40(1):1–14

    Google Scholar 

  • Chen QL, Ma CL (1998) Myxozoa: Myxosporea. Science Press, Beijing (in Chinese with English summary)

    Google Scholar 

  • Chen XL, Zhou WM (1998) Two new species of Myxosporidia from the fishes (Myxosporidia: Myxosomatidae: Myxobolidae). Acta Zootax Sin 23:113–116 (in Chinese with English summary)

    Google Scholar 

  • Eszterbauer E, Benko M, Dan A (2001) Identification of fish parasitic Myxobolus (Myxosporea) species using a combined PCR-RFLP method. Dis Aquat Org 44:35–39

    Article  PubMed  CAS  Google Scholar 

  • Ferguson JA, Atkinson SD, Whipps CM, Kent ML (2008) Molecular and morphological analysis of Myxobolus spp. of salmonid fishes with the description of Myxobolus fryeri n. sp. J Parasitol 94:1322–1334

    Article  PubMed  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Liu Y, Gu ZM, Luo YL (2010a) Some additional data to the occurrence, morphology and validity of Myxobolus turpisrotundus Zhang, 2009 (Myxozoa: Myxosporea). Parasitol Res 107:67–73

    Article  PubMed  CAS  Google Scholar 

  • Liu Y, Whipps CM, Gu ZM, Zeng LB (2010b) Myxobolus turpisrotundus (Myxosporea: Bivalvulida) spores with caudal appendages: investigating the validity of the genus Henneguya with morphological and molecular evidence. Parasitol Res 107:699–706

    Article  PubMed  Google Scholar 

  • Lom J, Arthur JR (1989) A guideline for preparation of species descriptions in Myxosporea. J Fish Dis 12:151–156

    Article  Google Scholar 

  • Lom J, Dyková I (2006) Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol 53:1–36

    PubMed  Google Scholar 

  • Lom J, Noble ER (1984) Revised classification of the class Myxosporea Bütschli, 1881. Folia Parasitol 31:193–205

    Google Scholar 

  • Lu YS, Nie P (2004) Phylogenetic analysis of the myxosporeans parasitic in freshwater fish of China based on 18S ribosomal DNA. Acta Hydrobiol Sin 28:587–591 (in Chinese with English summary)

    CAS  Google Scholar 

  • Luo F, Zhang QZ (1999) Investigation on Myxosporidia fauna from Cyprinus carpio (Linnaeus) and Carassius auratus auratus (Linnaeus) cultivated in Beibei district, Chongqing. J Ningde Teach Coll (Nat Sci) 11:97–100 (in Chinese with English summary)

    Google Scholar 

  • Luo F, Zhang QZ (2001) Notes on a new species of Myxosporidia from Cyprinus carpio Linnaeus (Myxosporea: Bivalvulida). Acta Zootax Sin 26:162–165 (in Chinese with English summary)

    Google Scholar 

  • Molnár K, Eszterbauer E, Székely C, Dan A, Harrach B (2002) Morphological and molecular biological studies on intramuscular Myxobolus spp. of cyprinid fish. J Fish Dis 25:643–652

    Article  Google Scholar 

  • Molnár K, Marton S, Eszterbauer E, Székely C (2006) Comparative morphological and molecular studies on Myxobolus spp. infecting chub from the River Danube, Hungary, and description of M. muellericus sp. n. Dis Aquat Org 73:49–61

    Article  PubMed  Google Scholar 

  • Molnár K, Székely C, Hallett SL, Atkinson SD (2009) Some remarks on the occurrence, host-specificity and validity of Myxobolus rotundus Nemeczek, 1911 (Myxozoa: Myxosporea). Syst Parasitol 72:71–79

    Article  PubMed  Google Scholar 

  • Whipps CM, Diggles BK (2006) Kudoa alliaria in flesh of Argentinian hoki Macruronus magellanicus (Gadiformes; Merlucciidae). Dis Aquat Org 69:259–263

    Article  PubMed  Google Scholar 

  • Whipps CM, Kent ML (2006) Phylogeography of the cosmopolitan marine parasite Kudoa thyrsites (Myxozoa: Myxosporea). J Eukaryot Microbiol 53:364–474

    Article  PubMed  CAS  Google Scholar 

  • Whipps CM, El-Matbouli M, Hedrick RP, Blazer V, Kent ML (2004) Myxobolus cerebralis internal transcribed spacer 1 (ITS-1) sequences support recent spread of the parasite to North America and within Europe. Dis Aquat Org 60:105–108

    Article  PubMed  CAS  Google Scholar 

  • Zhang QZ (2001) A new species of the genus Myxobolus parasitic in the fish Carassius auratus auratus. Acta Hydrobiol Sin 25:279–281 (in Chinese with English summary)

    Google Scholar 

  • Zhang JY, Yokoyama H, Wang JG, Li AH, Gong XN, Ryu-Hasegawa A, Iwashita M, Ogawa K (2010) Utilization of tissue habitats by Myxobolus wulii Landsberg & Lom, 1991 in different carp hosts and disease resistance in allogynogenetic gibel carp: redescription of M. wulii from China and Japan. J Fish Dis 33:57–68

    Article  PubMed  CAS  Google Scholar 

  • Zhao Y, Sun C, Kent ML, Deng J, Whipps M (2008) Description of a new species of Myxobolus (Myxozoa: Myxobolidae) based on morphological and molecular data. J Parasitol 94:737–742

    PubMed  CAS  Google Scholar 

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The authors thank Gao Yu for his help with the drawing of spores. We also would like to thank an anonymous reviewer for the assistance in adapting our manuscript to the English language. This study was supported by the Nature Science Foundation of China (Project Number 30800096), New Teacher Foundation of Chinese Education Department (Project Number 200805041025), Natural Science Foundation of Hubei Province (Project Number U2008CDB078), Huazhong Agricultural University Scientific & Technological Self-innovation Foundation (Project Number 2009SC017), and Open Fund of Key Lab of Freshwater Biodiversity Conservation and Utilization (Project Number LFBCU0701).

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Correspondence to Zemao Gu.

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Liu, Y., Gu, Z., Zhang, Y. et al. Redescription and molecular analysis of Myxobolus shantungensis Hu, 1965 (Myxozoa: Myxosporea) infecting common carp Cyprinus carpio haematopterus . Parasitol Res 109, 1619–1623 (2011).

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