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Redescription and molecular analysis of Myxobolus shantungensis Hu, 1965 (Myxozoa: Myxosporea) infecting common carp Cyprinus carpio haematopterus

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Abstract

Myxobolus shantungensis Hu, 1965 infects gill arches of common carp Cyprinus carpio haematopterus (Temminck and Schlegel), causing serious pathological effects on host fish. An inadequate original description and absence of molecular data make accurate early diagnosis challenging. To augment the original description, M. shantungensis is redescribed here using morphological and molecular biological methods. Mature spores of M. shantungensis were ellipsoidal or apple shaped in frontal view and lemon shaped in lateral view, averaging 8.2 ± 0.3 μm (8.0–9.0 μm) × 10.1 ± 0.5 μm (9.2–11.1 μm) × 6.9 ± 0.3 μm (6.0–7.4 μm). Some spores had three to four “V”-shaped valve edge markings on the posterior of the spore. The two equal polar capsules were oval, measuring 4.3 ± 0.3 μm (3.8–5.0 μm) × 3.2 ± 0.2 μm (2.8–3.5 μm), situated at the anterior extremity of the spore. Polar filaments coiled with six to seven turns. Scanning electron microscopy revealed that the surface of mature spores of M. shantungensis was generally pitted with a number of irregular ridges in shape. M. shantungensis is also characterized on the molecular level using the sequence of the small subunit ribosomal RNA gene. A BLAST search revealed that this sequence did not match any available sequences in GenBank.

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Acknowledgments

The authors thank Gao Yu for his help with the drawing of spores. We also would like to thank an anonymous reviewer for the assistance in adapting our manuscript to the English language. This study was supported by the Nature Science Foundation of China (Project Number 30800096), New Teacher Foundation of Chinese Education Department (Project Number 200805041025), Natural Science Foundation of Hubei Province (Project Number U2008CDB078), Huazhong Agricultural University Scientific & Technological Self-innovation Foundation (Project Number 2009SC017), and Open Fund of Key Lab of Freshwater Biodiversity Conservation and Utilization (Project Number LFBCU0701).

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Correspondence to Zemao Gu.

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Liu, Y., Gu, Z., Zhang, Y. et al. Redescription and molecular analysis of Myxobolus shantungensis Hu, 1965 (Myxozoa: Myxosporea) infecting common carp Cyprinus carpio haematopterus . Parasitol Res 109, 1619–1623 (2011). https://doi.org/10.1007/s00436-011-2450-0

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