Biochemical profile of Biomphalaria glabrata (Mollusca: Gastropoda) after infection by Echinostoma paraensei (Trematoda: Echinostomatidae)
- 166 Downloads
The effect of infection by Echinostoma paraensei on the activity of the enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the concentration of total proteins, uric acid and urea in the hemolymph of Biomphalaria glabrata were investigated after exposure to five or 50 miracidia. The biochemical concentrations were measured weekly until the end of the fourth week after exposure. There was a significant decrease in the concentrations of total proteins in the snails exposed both to five and 50 miracidia, as well as an increase in the nitrogenous products of excretion, ALT and AST activities. The higher ALT activity in the hemolymph of the snails after infection with 50 miracidia suggests highest energetic requirement in these snails in relation to snails exposed to five miracidia. The results also suggest an increase in the use of total proteins, since there was increased formation of nitrogenous catabolites, in conformity with an increase in the aminotransferase activities, frequently associated with tissue damages. This can be explained by damage due to penetration by the miracidia and subsequent development of intramolluscan sporocysts and rediae.
KeywordsUric Acid Digestive Gland Total Protein Level Snail Host Infected Snail
This study was supported in part by Conselho Nacional para o Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ). The authors thank M.Sc. Juberlan Silva Garcia for technical assistance.
- Becker W, Schmale H (1975) The nitrogenous products of degradation—ammonia, urea and uric acid—in the hemolymph of the snail Biomphalaria glabrata. Z Parasitenkd 51A:407–411Google Scholar
- Bishop ML, Duben-Engelkirk JL, Fody EP (1996) Clinical chemistry. Principles, procedures, correlations, thirdth edn. Lippincott, FiladélfiaGoogle Scholar
- de Souza RM, Gomes EM, Chagas GM, Pinheiro J (2000) The Influence of starvation and Eurytrema coelomaticum infection on the nitrogenads produts of degradation in the hemolymph of Bradybaena similaris. Braz Arch Biol Technol 43:365–371Google Scholar
- Kaplan LA, Pesce AJ (1996) Clinical chemistry, Thirdth edn. Mosby, St. LouisGoogle Scholar
- Lustrino D, Tunholi-Alves VM, Tunholi VM, Bessa ECA, Pinheiro J (2008) Allamanda cathartica (Apocynaceae) seeds induces changes on carbohydrates deposits of Bradybaena similaris (Mollusca, Bradybaenidae). Rev Bras Zooc 10:23–27Google Scholar
- Maldonado A Jr, Loker ES, Morgan JAT, Rey L, Lanfredi RM (2001b) Description of the adult worms of a new Brazilian isolate of Echinostoma paraensei (Platyhelminthes: Digenea) from its natural vertebrate host Nectomys squamipes by light and scanning electron microscopy and molecular analysis. Parasitol Res 88:40–848Google Scholar
- Maldonado A Jr, Coura R, Garcia JG, Lanfredi RM, Rey (2001c) Changes on Schistosoma mansoni (Digenea: Schistosomatidae) worm load in Nectomys squamipes (Rodentia: Sigmodontidae) concurrently infected with Echinostoma paraensei (Digenea: Echinostomatidae). Mem Inst Oswaldo Cruz 96:193–198PubMedCrossRefGoogle Scholar
- Tunholi VM, Lustrino D, Tunholi-Alves VM, Mello-Silva CC, Maldonado A Jr, Rodrigues MD, Pinheiro J (2010) Changes in the reproductive biology of Biomphalara glabrata infected with different doses of Echinostoma paraensei miracidia. J Invertebr Pathol. doi: 10.1016/j.jip.2010.09.006 PubMedGoogle Scholar
- Weichselbaum CTE (1946) An accurate and rapid method for determination of proteins in small amounts of blood serum and plasma. Am J Clin Pathol 16:40–49Google Scholar