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Drug-induced apoptosis of Echinococcus granulosus protoscoleces

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Abstract

Parasitic infection by Echinococcus granulosus in humans induces hydatidosis (echinococcosis), which is a zoonotic disease that seriously endangers public health. This study was to determine the status of cell apoptosis in the protoscoleces of E. granulosus, which were isolated from hydatid cysts in livers or lungs of sheep. Those protoscoleces were incubated with drugs (at the concentration of 1 mmol L−1 H2O2 and 5 mmol L−1dexamethasone) for 8 h, the apoptosis were examined by transmission electron microscopy and TUNEL assay, the expression of caspase-1 and caspase-3 were detected by immunohistochemistry and caspase-3 activeity was detected by colorimetric assay. Our results have clearly demonstrated the presence of cell apoptosis in protoscoleces in the absence or presence of drug (H2O2, dexamethasone) treatment, but drug-induced apoptosis rate, caspase-1 and caspase-3 expression levels were higher than no-drug induce and caspase-3 activity were significantly increasing. We found H2O2 and dexamethasone can induce the cell apoptosis of protoscoleces. Our results implied the existence of a CED-3 like apoptosis gene in protoscolces and provide a rationale for further exploring the induction of apoptosis as non-surgical treatment method in treating this parasitic disease.

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Acknowledgments

This research was supported by the National Nature Science Foundation of China(#30560141)

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Correspondence to Jinfeng Kang.

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This research was supported by chinese natinal scientific fund, the project of the fund is the study on Hydatid induced apoptosis and its molecular mechanism. This Parasitic infection of Echinococcus in humans induces echinococcosis (hydatid disease), which is a zoonotic disease that seriously endangers public health. our aims of this study is to determine the status of cell apoptosis in Echinococcus granulosus by working with isolated protoscoleces. Results demonstrated clearly the presence of cell apoptosis in protoscoleces in the absence or presence of drug treatment and provide a rationale for further exploring the induction of apoptosis as non-surgical treatment method in treating this parasitic disease. The result of the research provided us a basement for futhur study on exploring the induction of apoptosis as a non-surgical treatment method in treating this parasitic disease.

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Hu, H., Kang, J., Chen, R. et al. Drug-induced apoptosis of Echinococcus granulosus protoscoleces. Parasitol Res 109, 453–459 (2011). https://doi.org/10.1007/s00436-011-2276-9

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  • DOI: https://doi.org/10.1007/s00436-011-2276-9

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