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Effects of platelet-activating factor on the interaction of Trypanosoma cruzi with Rhodnius prolixus

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Abstract

We investigated the effects of platelet-activating factor (PAF) on the interaction of Trypanosoma cruzi with Rhodnius prolixus. The parasites (epimastigotes) were treated with PAF and/or WEB 2086 (PAF antagonist) for 1 h prior to the interaction experiments. PAF stimulated both in vivo and ex vivo interactions between T. cruzi and R. prolixus while WEB 2086 abrogated these effects. PAF-treated epimastigotes also showed an increase in surface negativity and in the amount of surface sialic acid. Neither of these effects was observed when the epimastigotes were treated with neuraminidase following PAF treatment. In the ex vivo interaction experiments, the number of epimastigotes bound to the midguts of the insects was reduced when the epimastigotes had been treated with neuraminidase. We conclude that PAF modulates the interaction of T. cruzi with R. prolixus by altering the amount of sialyl residues at the surface of the parasite.

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Acknowledgements

We are thankful to Drs. Felipe A. Dias, Rafael Linden, Rossiane C. Vommaro, Adriane R. Todeschini, Lucia Mendonça-Previato, Jose O. Previato, and David Majerowicz for the helpful discussions and to Litiane M. Rodrigues and José de S. L. Junior for the technical assistance. The present work was supported by grants from the Brazilian Agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), and Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCTEM). All experiments complied with current Brazilian laws.

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The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Cidade Universitária, Ilha do Fundão, 21941-590, Rio de Janeiro, Rio de Janeiro, Brazil

    Luciana T. Zimmermann, Marta T. Gomes, Daniela S. Alviano, Celuta S. Alviano & Angela H. Lopes

  2. Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, Centro, 24020-141, Niterói, Rio de Janeiro, Brazil

    Evelize Folly

  3. Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Cidade Universitária, Ilha do Fundão, 21941-590, Rio de Janeiro, Rio de Janeiro, Brazil

    Fernando C. Silva-Filho

  4. Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Cidade Universitária, Ilha do Fundão, 21941-590, Rio de Janeiro, Rio de Janeiro, Brazil

    Marta T. Gomes & Geórgia C. Atella

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  1. Luciana T. Zimmermann
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  2. Evelize Folly
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  8. Angela H. Lopes
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Correspondence to Angela H. Lopes.

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Zimmermann, L.T., Folly, E., Gomes, M.T. et al. Effects of platelet-activating factor on the interaction of Trypanosoma cruzi with Rhodnius prolixus . Parasitol Res 108, 1473–1478 (2011). https://doi.org/10.1007/s00436-010-2194-2

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