Parasitology Research

, Volume 108, Issue 5, pp 1253–1263 | Cite as

Bioefficacy of Plumbago zeylanica (Plumbaginaceae) and Cestrum nocturnum (Solanaceae) plant extracts against Aedes aegypti (Diptera: Culicide) and nontarget fish Poecilia reticulata

  • Chandrashekhar D. Patil
  • Satish V. Patil
  • Bipinchandra K. Salunke
  • Rahul B. Salunkhe
Original Paper


In a search for natural products that could be used to control the vectors of tropical diseases, extracts of medicinal plants Plumbago zeylanica and Cestrum nocturnum have been tested for larvicidal activity against second, third, and fourth instar larvae of Aedes aegypti. The LC50 values of all the extracts in different solvents of both the plants were less than 50 ppm (15.40–38.50 ppm) against all tested larval instars. Plant extracts also affected the life cycle of A. aegypti by inhibition of pupal development and adult emergence with increasing concentrations. The larvicidal stability of the extracts at five constant temperatures (19°C, 22°C, 25°C, 28°C, and 31°C) evaluated against fourth instar larvae revealed that toxicity of both plant extracts increases with increase in temperature. Toxicity studies carried out against fish species Poecilia reticulata, the most common nontarget organism in the habitats of A. aegypti, showed almost nil to meager toxicity at LC50 and LC90 doses of the plant extracts. The qualitative analysis of crude extracts of P. Zeylanica and C. nocturnum revealed the presence of bioactive phytochemicals with predominance of plumbagin in P. zeylanica and saponins in C. nocturnum. Partially purified plumbagin from P. zeylanica and saponins from C. nocturnum were obtained, and their presence was confirmed by thin-layer chromatography and biochemical tests. The bioassay experiment of partially purified secondary metabolites showed potent mosquito larvicidal activity against the fourth instar larval form. Therefore, this study explored the safer and effective potential of plant extracts against vector responsible for diseases of public health importance.


Plant Extract Saponin Leaf Extract Root Extract Azadirachtin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Financial assistance from University Grant Commission (F.No. 34-539/2008 (SR) New Delhi, 2009) is gratefully acknowledged.


  1. Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Eco Entomol 18:265–266Google Scholar
  2. Ali A, Nayar JK, Xue RD (1995) Comparative toxicity of selected larvicides and insect growth regulators to a Florida laboratory, population of Aedes albopictus. J Am Mosq Control Assoc 11(1):72–76PubMedGoogle Scholar
  3. Amer A, Mehlhorn H (2006a) Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitol Res 99:466–472PubMedCrossRefGoogle Scholar
  4. Amer A, Mehlhorn H (2006b) Persistency of larvicidal effects of plant oil extracts under different storage conditions. Parasitol Res 99:473–477PubMedCrossRefGoogle Scholar
  5. Amer A, Mehlhorn H (2006c) Repellency effect of forty-one essential oils against Aedes, Anopheles and Culex mosquitoes. Parasitol Res 99:478–490PubMedCrossRefGoogle Scholar
  6. Bagavan A, Rahuman AA, Kamaraj C, Geetha (2008) Larvicidal activity of saponin from Achyranthes aspera against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 103:223–229PubMedCrossRefGoogle Scholar
  7. Bouchbaver G, Jirovetz L, Koul VK (1995) Volatiles of the absolute of Cestrum nocturnum L. J Essent Oil Res 7:5–9Google Scholar
  8. Chapagain BP, Wiesman Z, TsrorLahkim L (2007) In-vitro study of antifungal activity of saponin-rich extracts against prevalent phytopathogenic fungi. Ind Crops Prod 26:109–115CrossRefGoogle Scholar
  9. Chapagain BP, Saharan V, Wiesman Z (2008) Larvicidal activity of saponins from Balanites aegyptiaca callus against Aedes aegypti mosquito. Bioresour Technol 99:1165–1168PubMedCrossRefGoogle Scholar
  10. Chopra RN, Nayar SL, Chopra IC (1996) Glossary of Indian medicinal plants. NISCOM, New DelhiGoogle Scholar
  11. Chowdhury N, Chatterjee SK, Laskar S, Chandra G (2009) Larvicidal activity of Solanum villosum Mill (Solanaceae: Solanales) leaves to Anopheles subpictus Grassi (Diptera: Culicidae) with effect on non-target Chironomus circumdatus Kieffer (Diptera: Chironomidae). J Pest Sci 82:13–18CrossRefGoogle Scholar
  12. de Silva BP, de Sousa AC, Silva GM, Mendes TP, Parente JP (2002) A new bioactive steroidal saponin from Agave attenuata. Z Naturforsch 57(5–6):423–428Google Scholar
  13. Elango G, Rahuman AA, Bagavan A, Kamaraj C, Zahir AA, Venkatesan C (2009) Laboratory study on larvicidal activity of indigenous plant extracts against Anopheles subpictus and Culex tritaeniorhynchus. Parasitol Res 104:1381–1388PubMedCrossRefGoogle Scholar
  14. Eskander J, Lavaud C, Pouny I, Soliman HSM, Abdel-Khalik SM, Mahmoud II (2006) Saponins from the seeds of Mimusops laurifolia. Phytochemistry 67:1793–1799PubMedCrossRefGoogle Scholar
  15. Evans WC (1997) An index of medicinal plants. A text book of pharmacognosy, vol 7(5), 14th edn. J & A Churchill, London, pp 12–14Google Scholar
  16. Finney DJ (1971) Probit analysis. Cambridge University Press, Cambridge, pp 76–80Google Scholar
  17. Ghosh A, Chandra G (2006) Biocontrol efficacy of Cestrum diurnum L. (Solanaceae: Solanales) against the larval forms of Anopheles stephensi. Nat Prod Res 20:371–379PubMedCrossRefGoogle Scholar
  18. Ghosh A, Chowdhury N, Chandra G (2008) Laboratory evaluation of a phytosteroid compound of mature leaves of Day Jasmine (Solanaceae: Solanales) against larvae of Culex quinquefasciatus (Diptera: Culicidae) and nontarget organisms. Parasitol Res 103:271–277PubMedCrossRefGoogle Scholar
  19. Govindarajan M, Jebanesan A, Pushpanathan T (2008) Larvicidal and ovicidal activity of Cassia fistula Linn. leaf extract against filarial and malarial vector mosquitoes. Parasitol Res 102:289–292PubMedCrossRefGoogle Scholar
  20. Grantz GN (1993) What must we do to effectively control Aedes aegypti. J Trop Med 35:243–251Google Scholar
  21. Green MM, Singer JM, Sutherland DJ, Hibbon CR (1991) Larvicidal activity of Tagetes minuta (Marigold) towards Aedes aegypti. J Am Mosq Control Asso 7:282–286Google Scholar
  22. Harborne JB (1973) Phytochemical methods. Chapman and Hall, London, pp 49–188Google Scholar
  23. Jawale C, Kirdak R, Dama L (2010) Larvicidal activity of Cestrum nocturnum on Aedes aegypti. Bangladesh J Pharmacol 5:39–40Google Scholar
  24. Josephrajkumar A, Subrahmanym B (2002) DNA synthesis in the imaginal wing discs of the American bollworm Helicoverpa armigera (Hübner). J Biosci 27(2):113–120PubMedCrossRefGoogle Scholar
  25. Kamaraj C, Rahuman AA, Bagavan A (2008) Antifeedant and larvicidal effects of plant extracts against Spodoptera litura (F.), Aedes aegypti L. and Culex quinquefasciatus Say. Parasitol Res 103:325–331PubMedCrossRefGoogle Scholar
  26. Karlos ALR, MdC C, Maria TM, Estelita PL, Eulogio LM, José RMR (2009) Activities of naphthoquinones against Aedes aegypti (Linnaeus, 1762) (Diptera: Culicidae), vector of dengue and Biomphalaria glabrata (Say, 1818), intermediate host of Schistosoma mansoni. Acta Trop 111(1):44–50CrossRefGoogle Scholar
  27. Karunamoorthi K, Ramanujam S, Rathinasamy R (2008) Evaluation of leaf extracts of Vitex negundo L. (Family: Verbenaceae) against larvae of Culex tritaeniorhynchus and repellent activity on adult vector mosquitoes. Parasitol Res 103:545–550PubMedCrossRefGoogle Scholar
  28. Kaushik R, Saini P (2008) Larvicidal activity of leaf extract of Millingtonia hortensis (Family: Bignoniaceae) against Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti. J Vector Borne Dis 45:66–69PubMedGoogle Scholar
  29. Kharat S, Dahanukar N, Raut R, Mahabaleshwarka M (2003) Long-term changes in fresh water fish species composition in North Western Ghats, Pune district. Curr Sci 84(6):816–820Google Scholar
  30. Kokate A (1999) Phytochemical methods. Phytotherapy 78:126–129, 2nd editionGoogle Scholar
  31. Mace GSL (1963) Anaerobic bacteriology for clinical laboratories. Pharmacognosy 23:89–91Google Scholar
  32. Mathew N, Anitha MG, Bala TSL, Sivakumar SM, Narmadha R, Kalyanasundaram M (2009) Larvicidal activity of Saraca indica, Nyctanthes arbor-tristis, and Clitoria ternatea extracts against three mosquito vector species. Parasitol Res 104:1017–1025PubMedCrossRefGoogle Scholar
  33. Mehlhorn H, Schmahl G, Schmidt J (2005) Extract of the seeds of the plant Vitex agnus castus proven to be highly efficacious as a repellent against ticks, fleas, mosquitoes and biting flies. Parasitol Res 95:363–365PubMedCrossRefGoogle Scholar
  34. Mimaki Y, Wantanabe K, Sakagami H, Sashida Y (2002) Steroidal glycosides from the leaves of Cestrum nocturnum. J Nat Prod 65:1863–1868PubMedCrossRefGoogle Scholar
  35. Ntonifor NN, Ngufor CA, Kimbi HK, Oben BO (2006) Traditional use of mosquito repellent to protect human against mosquito and other insect bites in rural community of Cameroon. East Afr Med J 83:553–558PubMedGoogle Scholar
  36. Okumu OF, Knols GJB, Fillinger U (2007) Larvicidal effects of a neem (Azadirachta indica) oil formulation on the malaria vector Anopheles gambiae. Malar J 6:63PubMedCrossRefGoogle Scholar
  37. Parimala R, Sachdanandam P (1993) Effect of Plumbagin on some glucose metabolising enzymes studied in rats in experimental hepatoma. Mol Cell Biochem 125:59–63PubMedCrossRefGoogle Scholar
  38. Patil SV, Patil CD, Salunke BK, Salunkhe RB (2010a) Larvicidal efficacy of six plants against two mosquito species Aedes aegypti and Anopheles stephensi. Trop Biomed 27(3) (in press)Google Scholar
  39. Patil SV, Salunke BK, Patil CD, Salunkhe RB (2010b) Potential of extracts of the tropical plant Balanites aegyptiaca (L) Del. (Balanitaceae) to control the mealybug, Maconellicoccus hirsutus (Homoptera: Pseudococcidae). Crop Prot 29:1293–1296CrossRefGoogle Scholar
  40. Pavela R (2009) Larvicidal property of essential oils against Culex quinquefasciatus Say (Diptera: Culicidae). Ind Crops Prod 30(2):311–315CrossRefGoogle Scholar
  41. Pelah D, Abramovich Z, Markus A, Wiesman Z (2002) The use of commercial saponin from Quillaja saponaria bark as a larvicidal agent against Aedes aegypti and Culex pipiens. J Ethnopharmacol 81:407–409PubMedCrossRefGoogle Scholar
  42. Pizarro AP, Oliveira Filho AM, Parente JP, Melo MT, dos Santos CE, Lima PR (1999) Utilization of the waste of sisal industry in the control of mosquito larvae. Rev Soc Bras Med Trop 32(1):23–29PubMedCrossRefGoogle Scholar
  43. Promsiri S, Naksathit A, Kruatrachue M, Thavara U (2006) Evaluations of larvicidal activity of medicinal plant extracts to Aedes aegypti (Diptera: Culcidae) and other effects on a non target fish. Insect Sci 13:179–188CrossRefGoogle Scholar
  44. Rahuman RR, Venketesan P (2008) Larvicidal efficacy of five cucurbitaceous plant leaf extracts against mosquito species. Pararsitol Res 103:133–139CrossRefGoogle Scholar
  45. Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K (2008) Isolation and identification of mosquito larvicidal compound from Abutilon indicum (Linn.) Sweet. Parasitol Res 102(5):981–988PubMedCrossRefGoogle Scholar
  46. Rahuman AA, Bagavan A, Kamaraj C, Saravanan E, Zahir AA, Elango G (2009a) Efficacy of larvicidal botanical extracts against Culex quinquefasciatus Say (Diptera: Culicidae). Parasitol Res 104:1365–1372PubMedCrossRefGoogle Scholar
  47. Rahuman AA, Bagavan A, Kamaraj C, Vadivelu M, Zahir AA, Elango G, Pandiyan G (2009b) Evaluation of indigenous plant extracts against larvae of Culex quinquefasciatus Say (Diptera: Culicidae). Parasitol Res 104:637–643PubMedCrossRefGoogle Scholar
  48. Rajkumar S, Jebanesan A (2005) Larvicidal and adult emergence inhibition effect of Centella asiatica Brahmi (Umbelliferae) against mosquito Culex quinquefasciatus Say (Diptera: Culcidae). Afr J Biomed Res 8:31–33Google Scholar
  49. Rivera AE, Gattuso M, Alvarado R, Zárate E, Agüero J, Feria I, Lozoya X (2007) Pharmacognostical studies of the plant drug Mimosae tenuiflorae cortex. J Ethnopharmacol 113(3):400–408Google Scholar
  50. Rodrigues AMS, Paula de JE, Roblot F, Fournet A, Espíndola LS(2005) Larvicidal activity of Cybistax antisyphilitica against Aedes aegypti larvae. Fitoterapia 76(7–8):755–757Google Scholar
  51. Rongsriyam Y, Baskoro T (1998) Medicinal plants for replacement of insecticides used in vector control. Research Abstract, Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok. pp. 683Google Scholar
  52. Shaalan EAS, Canyonb D, Younesc MWF, Abdel-Wahaba H, Mansoura AH (2005) A review of botanical phytochemicals with mosquitocidal potential. Environ Int 31:1149–1166Google Scholar
  53. Sharif M, Rahman A, Kang SC (2009) Chemical composition and inhibitory effect of essential oil and organic extracts of Cestrum nocturnum L. on food-borne pathogens. Int J Food Sci Tech 44:1176–1182Google Scholar
  54. Selvakumar V, Anbudurai PR, Balakumar T (2001) In vitro Propagation of the Medicinal Plant Plumbago zeylanica L through Nodal Explants. in vitr cell deve plan 37(2):280–284Google Scholar
  55. Sivagnaname N, Kalyanasundaram M (2004) Laboratory evaluation of methanolic extract of Atlantia monophylla (Family: Rutaceae) against immature stages of mosquitoes and non-target organism. Mem Inst Oswaldo Cruz 99(1):115–118Google Scholar
  56. Senthilkumar N, Varma P, Gurusubramanian G (2009) Larvicidal and adulticidal activities of some medicinal plants against the malarial vector, Anopheles stephensi (Liston). Parasitol Res 104:237–244PubMedCrossRefGoogle Scholar
  57. Sukumar K, Perich MJ, Boobar LR (1991) Botanical derivatives in mosquito control: a review. J Am Mosq Control Assoc 7:210–217Google Scholar
  58. Tilak JC, Adhikari S, Thomas PA, Devasagayam (2004) Antioxidant properties of Plumbago zeylanica: an Indian medicinal plant and its active ingredient Plumbagin. Redox Report 9:219Google Scholar
  59. Treyvaud V, Marston A, Dyatmiko W, Hostettmann K (2000) Molluscicidal saponins from Phytolacca icosandra. Phytochemistry 55:603–609Google Scholar
  60. WHO (1996) Report of the WHO informal consultation on the evaluation on the testing of insecticides CTD/WHO PES/IC/96.1:69Google Scholar
  61. Wiseman Z, Chapagain BP (2006) Larvicidal activity of saponin containing extracts and fractions of fruit mesocarp of Balanites aegyptiaca. Fitoterapia 77:420–424Google Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Chandrashekhar D. Patil
    • 1
  • Satish V. Patil
    • 1
    • 2
  • Bipinchandra K. Salunke
    • 1
  • Rahul B. Salunkhe
    • 1
  1. 1.School of Life SciencesNorth Maharashtra UniversityJalgaonIndia
  2. 2.North Maharashtra Culture Collection CentreNorth Maharashtra UniversityJalgaonIndia

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