Abstract
Different MHC haplotype of Kazakh sheep has different resistance and susceptibility of hydatidosis. Notably, the MvaIbc-SacIIab-Hin1Iab haplotype of MHC-DRB1 exon two was associated with resistance hydatidosis. In order to analyze the antibody and cytokine responses to hydatidosis in Kazakh sheep with hydatidosis resistance haplotype, eight Kazakh sheep with the haplotype of MvaIbc-SacIIab-Hin1Iab were chosen as the test group, and other eight, which were not associated with hydatidosis resistance or susceptibility, were taken as control. After experimentally infected with hydatid orally, the blood was collected on 0, 7, 14, 30, 45, 60, 75, 90, 105, and 120 days. Serum and mRNA level of the cytokines IL-2, IFN-γ, TNF-α, IL-4, and IL-10 were evaluated by ELISA and fluorescence quantitative real-time polymerase chain reaction, respectively. The total white blood cells and leukomonocytes were determined by automation cytoanalyze. The level of IgE, IgG, and IgM were evaluated by ELISA. The results showed that the total white blood cells and leukomonocytes in test group were significantly higher than in control on 7, 45, 90, and 105 days post-infection (p.i.). The serum level of IL-2 in test group was significantly higher than in control on 45 days p.i., while the difference of IL-2 mRNA expression between test and control group was not significant. The serum level of TNF-α in test group was significantly higher than in control at 90 and 105 days p.i., and the TNF-α mRNA in test group was also significantly higher than in control on 90 days p.i. The level of IgE, IgG, and IgM in test group was higher than in control, but none was significant. The results suggested that the test group, which was predominant of Th1, could induce the protective immunity, while the control, which was predominant of Th2, could induce the susceptibility to infection of hydatidosis.
Similar content being viewed by others
References
Abdul-Basit A, Al-Ghoury, Eman M, El-Hamshary (2010) HLA class II alleles: susceptibility or resistance to cystic echinococcosis in Yemeni patients. Parasitol Res 107:355–361
Adeli, Ayiguli, Chen WG (2009) The suggestions about resources and utilizing of Hazakah sheep. XinJiang animal husbandry 1:48–49, China
Andersen FL, Ouhelli H, Kashani M (1997) Compendium on cystic echinococcosis. Brigham Young University, Provo, UT 84602, USA
Bauder B, Auer H, Schilcher F, Gabler C, Romig T, Bilger B, Aspock H (1999) Experimental investigations on the B and T cell immune response in primary alveolar echinococcosis. Parasite Immunol 21:409–421
Bresson-Hadni, Liance SM, Meyer JP, Houin R, Bresson JL, Vuitton DA (1990) Cellular immunity in experimental Echinococcus multilocularis infection. II. Sequential and comparative phenotypic study of the periparasitic mononuclear cells in resistant and sensitive mice. Clin Exp Immunol 82:378–383
Craig PS (1986) Detection of specific circulating antigen, immune complexes and antibodies in human hydatidosis from Turkana (Kenya) and Great Britain, by enzyme immunoassay. Parasite Immunol 8:171–188
Daeki AO, Craig PS, Shambesh MK (2000) IgG-subclass antibody responses and the natural history of hepatic cystic echinococcosis in asymptomatic patients. Ann Trop Med Parasitol 94:319–328
Dalimi A, Motamedi G, Hosseini M, Mohammadian B, Malaki H, Ghamari Z, Ghaffari FF (2002) Echinococcosis/hydatidosis in western Iran. Vet Parasitol 105(2):161–171
Dematteis S, Rottenberg M, Baz A (2003) Cytokine response and outcome of infection depends on the infective dose of parasites in experimental infection by Echinococcus granulosus. Parasite Immunol 25:189–219
Dessaint JP, Bout D, Wattre P, Capron A (1975) Quantitative determination of specific IgE antibodies to Echinococcus granulosus and IgE levels in sera from patients with hydatid disease. Immunology 29:813–823
Dixon JB, Jenkin PS (1995) Immunology of mammalian metacestode infections. II. Immune recognition and effector function. Helminthol 64:599–613
Eckert J, Deplazes P (2004) Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev 17(1):107–135
Eiermann TH, Bettens F, Tiberghien P (1998) HLA and alveolar echinococcosis. Tissue Antigens 52(2):124–129
Emery I, Liance M, Deriaud E, Vuitton DA, Houin R, Leclerc C (1996) Characterization of T-cell immune responses of Echinococcus multilocularis -infected C57BL/6 J mice. Parasite Immunol 18:463–472
Emery I, Liance M, Leclerc CC (1997) Secondary Echinococcus multilocularis infection in A/J mice: delayed metacestode development is associated with Th1 cytokine production. Parasite Immunol 19:493–503
Fang YQ, Tan Y, Yang GZ (1999) The effects of IL-2 against toxoplasma gondii infection in vivo. Immunol J 115(1):32–34
Fotiadis C, Sergiou C, Kirou J, Troupis TG, Tselentis J, Doussaitou P, Gorgoulis VG, Sechas MN (1999) Experimental Echinococcus infection in the mouse model: pericystic cellular immunity reaction and effects on the lymphoid organs of immunocompetent and thymectomized mice. In Vivo 13:541–546
Godot V, Harraga S, Beurton I (2000) Resistance/susceptibility to Echinococcus multilocularis infection and cytokine profile in humans. II. Influence of the HLA B8, DR3, DQ2 haplotype. Clin Exp Immunol 121:491–498
Gottstein B, Hemphill A (1997) Immunopathology of echinococcosis. Chem Immunol 66:177–208
Gottstein B, Bettens F (1994) Association between HLA-DR13 and susceptibility to alveolar echinococcosis. J Infect Dis 169:1416–1423
Hashemi Tabar GR, Borji H (2010) Antibody responses to hydatid cyst in experimentally infected lambs. World Appl Sci J 8(8):1001–1006
Hill AVS, Allsopp CEM, Kwiatkowski D (1991) Common West African HLA antigens are associated with protection from severe malaria. Nature 352:595–600
Jenkins DJ, Romig T, Thompson RCA (2005) Emergence re-emergence of Echinococcus spp.a global update. Int J Parasitol 35:1205–1219
Jiang CP (2001) Parasite infection and cytokine. Chin J Parasit Dis Con 14(2):150–153
Khabiri AR, Bagheri F, Assmar M, Siavashi MR (2006) Analysis of specific IgE and IgG subclass antibodies for diagnosis of Echinococcus granulosus. Parasite Immunol 28:357–362
Khaled M, Al-Qaoud, Sami K, Abdel-Hafez (2008) The induction of T helper type 1 response by cytokine gene transfection protects mice against secondary hydatidosis. Parasitol Res 102:1151–1155
Li Y, Yan JL, Li J (2005) Investigation and analysis of epidemic conditions of hydatidosis disease of sheep in Xinjiang. J Shihezi UniversityNatural Sci 23:60–64, China
Meyer CG, Gallin M, Erttmann KD (1994) HLA-D alleles associated with generalized disease, localized disease, and putative immunity in Onchocerca volvulus infection. Proc Natl Acad Sci USA 91:7515–7524
Moore KW, deWaal Malefyt R, Coffman RL, O’Garra A (2001) Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol 19:683–765
Rausch RL (1995) Life cycle patterns and geographic distribution of Echinococcus species. In: Thompson RCA, Lymbery AJ (eds) Echinococcus and hydatid disease CAB International. Oxon, UK
Pinon JM, Poirriez J, Lepan H, Geers R, Penna R, Fernandez D (1987) Value of isotypic characterization of antibodies to Echinococcus granulosus by enzyme-linked immuno-filtration assay. Eur J Clin Microbiol 6:291–295
Rogan MT (1987) Echinococcus granulosus. Studies on the development of the metacestode tegument. University of Salford, UK
Rogan MT, Craig PS, Zehyle E, Masinde G, Wen H, Zhou P (1992) In vitro killing of taeniid oncospheres, mediated by human sera from hydatid endemic areas. Acta Trop 51:291–296
Rigano R, Buttari B, Profumo E, Ortona E, Delunardo F, Margutti P, Mattei V, Teggi A, Sorice M, Siracusano A (2007) Echinococcus granulosus antigen B impairs human dendritic cell differentiation and polarizes immature dendritic cell maturation towards a Th2 cell response. Infect Immun 75:1667–1678
Li RY, Jia B, Zhang WJ, Zhao ZS, Shi GQ, Shen H, Peng Q (2010) Analysis of the Relationship between MHC-DRB1 gene polymorphism and hydatidosis in Kazakh sheep. Asian Aust J Anim Sci 23(9):1145–1151
Sterla S, Sato H, Nieto A (1999) Echinococcus granulosus human infection stimulates low avidity anticarbohydrate IgG2 and high avidity antipeptide IgG4 antibodies. Parasite Immunol 21:27–34
Touil-Boukoffa C, Sanceau J, Tayebi B, Wietzerbin J (1997) Relationship among circulating interferon, tumor necrosis factor-α, and interleukin-6 and serologic reaction against parasitic antigen in human hydatidosis. J Interferon Cytokine Res 17:211–217
Vuitton DA (2003) The ambiguous role of immunity in echinococcosis: protection of the host or of the parasite? Acta Trop 85:119–132
Zhang WB, Allen GR, Donald PM (2008) Mechanisms of immunity in hydatid development disease: implications for vaccine. J Immunol 181:6679–6685
Wei XL, Ding JB, Xu Y, Wen H, Lin RY (2004) Change of cytokines in mice with Echinococcus multilocularis infection. Chin J Parasitol Parasit Dis 22(6):361–364
Zhang W, Li J, McManus DP (2003) Concepts in immunology and diagnosis of hydatid disease. Clin Microbiol Rev 16:18–36
Acknowledgments
We thank all the people who helped us for the present study. This study was supported by a grant from the Natural Science Foundation of China (30660124) to Prof. Bin Jia.
Author information
Authors and Affiliations
Corresponding author
Additional information
Ren-Yan Li and Qiang Peng contributed equally to this article.
Rights and permissions
About this article
Cite this article
Li, RY., Peng, Q., Jia, B. et al. Antibody and cytokine responses to hydatid in experimentally infected Kazakh sheep with hydatidosis resistance haplotype. Parasitol Res 108, 1131–1137 (2011). https://doi.org/10.1007/s00436-010-2155-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-010-2155-9