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Impact of Parthenium hysterophorus leaf extracts on the fecundity, fertility and behavioural response of Aedes aegypti L.

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Abstract

Different extracts of 1,000 ppm were prepared from the leaves of Parthenium hysterophorus using acetone, benzene, petroleum ether, diethyl ether and hexane as the solvents. The efficacy of each extract was assessed against dengue fever vector, Aedes aegypti by evaluating the variations in fecundity, fertility and behavioural response of the female adults. The leaf extracts could cause 70–100% repellency in the oviposition behaviour of the adults. The diethyl ether extract was found to be the most effective extract resulting in maximum effective repellency (99.7%) leading to the highest levels of reduced fecundity and 100% egg mortality followed by benzene extracts causing 93.8% reduced oviposition and 100% ovicidal effect. Hexane and acetone extracts with the least oviposition deterrence of 70–74% and negligible egg mortality (8–9%) proved to be the least effective extracts. The petroleum ether extract had a moderate impact resulting in 93.2% diminished fecundity and 41% ovicidal effect. The behavioural response of female adults of A. aegypti was evaluated by performing spatial repellency and contact irritancy assays. The most significant spatial repellency behaviour was elicited by acetone extracts leading to escape of 80% mosquitoes. Hexane and diethyl ether extracts could cause moderate response with 50–60% escape, while a slight and no reaction was observed on exposure to petroleum ether and benzene extracts, respectively. An interesting observation was the knocked-down activity caused by the hexane extracts with no recovery even after 24 h. A significant contact irritancy response was noticed in the mosquitoes on exposure to acetone leaf extracts resulting in first flight only after 4 s and a total of 12 flights during exposure. No irritancy behaviour was observed on exposure to diethyl ether and benzene leaf extracts. However, as against controls, a slight irritability response was noticed on exposure to hexane leaf extracts resulting in relative irritability of 1.2. Our results suggest the selective efficiency of Parthenium leaf extracts against A. aegypti, as the most effective oviposition deterrent and ovicidal agent was least effective as irritant extract and vice-versa. Further detailed research is needed to identify the active ingredient in the extracts and implement the effective mosquito management programme.

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Acknowledgements

The authors are thankful to Department of Science and Technology for providing financial assistance to our project. The authors extend thanks to Dr. Savithri Singh, Principal, Acharya Narendra Dev College for providing the laboratory and culture facilities to conduct the experimental work.

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  1. Department of Zoology, Acharya Narendra Dev College University of Delhi, Kalka Ji, New Delhi, 110019, India

    Sarita Kumar, Abhay Pratap Singh, Gokul Nair, Sahil Batra, Anubha Seth, Naim Wahab & Radhika Warikoo

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  1. Sarita Kumar
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  2. Abhay Pratap Singh
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Correspondence to Sarita Kumar.

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Kumar, S., Singh, A.P., Nair, G. et al. Impact of Parthenium hysterophorus leaf extracts on the fecundity, fertility and behavioural response of Aedes aegypti L.. Parasitol Res 108, 853–859 (2011). https://doi.org/10.1007/s00436-010-2126-1

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