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Kinetics of circulating antigen 14-3-3 in sera of rabbits firstly infected with Schistosoma japonicum and treated with/without praziquantel

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A sandwich ELISA was developed for the detection of circulating antigen 14-3-3 in the sera of rabbits. Rabbits that were infected with 500 cercariae of Schistosoma japonicum were grouped and the kinetics of 14-3-3 was observed. For the treated group, the 14-3-3 protein could be detected as early as 2–4 weeks postinfection and then its levels rose rapidly and reached a peak at around 6 weeks. The 14-3-3 levels in the sera significantly decreased after the infected rabbits were treated with praziquantel at 6 weeks postinfection and declined to the initial level about 8 weeks posttreatment. While in the untreated group, 14-3-3 levels reached a peak in 8 weeks postinfection and then remained at plateau level for about 6 weeks. Our findings showed that detection of S. japonicum14-3-3 has an important value for diagnosis of acute infection of S. japonicum and evaluation of chemotherapy.

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This work was supported by the grants from Science and Technology Foundation of Jiangsu Provincial Bureau of Health (grant no. H200738) and National Project of Important Infectious Diseases (grant no. 2008ZX10004-011).

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Correspondence to Chuan-Xin Yu.

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Qian, CY., Song, LJ., Hua, WQ. et al. Kinetics of circulating antigen 14-3-3 in sera of rabbits firstly infected with Schistosoma japonicum and treated with/without praziquantel. Parasitol Res 108, 493–495 (2011).

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