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Acaricidal effect and chemical composition of essential oils extracted from Cuminum cyminum, Pimenta dioica and Ocimum basilicum against the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)

Parasitology Research volume 108, pages 481–487 (2011)Cite this article

Abstract

Acaricidal activity of essential oils extracted from cumin seeds (Cuminum cyminum), allspice berries (Pimenta dioica) and basil leaves (Ocimum basilicum) were tested on 10-day-old Rhipicephalus (Boophilus) microplus tick larvae using the LPT. Two-fold dilutions of the three essential oils were tested from a starting dilution of 20% down to 1.25%. Results showed a high toxicological effect for cumin, producing 100% mortality in all tested concentrations on R. microplus larvae. Similarly, allspice essential oil produced 100% mortality at all concentrations with the exception of a dramatic decrease at 1.25% concentration. Conversely, basil essential oil was not shown to be toxic against R. microplus larvae. The most common compounds detected by gas chromatography-mass spectrometry were as follows: cumin: cuminaldehyde (22.03%), γ-terpinene (15.69%) and 2-caren-10-al (12.89%); allspice: methyl eugenol (62.7%) and eugenol (8.3%); basil: linalool (30.61%) and estragole (20.04%). Results clearly indicate that C. cyminum and P. dioica essential oils can be used as an effective alternative for R. microplus tick control, and there is a high probability they can be used for other ticks affecting cattle in Mexico and throughout the world, thereby reducing the necessity for traditional and unfriendly synthetic acaricides.

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References

  • Aguirre EJ, Aburto S (1983) Determinación de las concentraciones discriminantes como medio de diagnóstico de susceptibilidad en garrapatas. B. microplus. In: IV Reunión Anual de Parasitología Veterinaria, México, D.F., 2–6 de septiembre de 1983, pp 6–10

  • Borges LMF, Ferri PH, Silva WJ, Silva WC, Silva JG (2003) In vitro efficacy of extracts of Melia azedarach against the tick Boophilus microplus. Med Vet Entomol 17:228–231

    Article  CAS  PubMed  Google Scholar 

  • Boyraz N, Özcan M (2005) Antifungal effect of some spice hydrosols. Fitoterapia 76:661–665

    Article  PubMed  Google Scholar 

  • Brown HA, Minott DA, Ingram CW, Williams LAD (1998) Biological activities of the extracts and constituents of pimento, Pimenta dioica L. against the southern cattle tick, Boophilus microplus. Insect Sci Appl 18:9–16

    CAS  Google Scholar 

  • Cetin H, Cilek JE, Aydin L, Yanikoglu A (2009) Acaricidal effects of the essential oil of Origanum minutiflorum (Lamiaceae) against Rhipicephalus turanicus (Acari: Ixodidae). Vet Parasitol 160:359–361

    Article  CAS  PubMed  Google Scholar 

  • Chaubey MK (2008) Fumigant toxicity of essential oils from some common spices against pulse bettle, Callosobruchus chinensis (Coleoptera: Bruchidae). J Oleo Sci 57:171–179

    CAS  PubMed  Google Scholar 

  • Chittihunsa T, Samngamnim N (1999) Studies on the effects of some crude plant extracts to the mortality and feeding behavior of Nephotettix virescens. In: Oates CG (ed) The 37th Kasetsart University Annual Conference, Bangkok, Thailand, 3–5 February 1999. Text and J Publication Co, Ltd., pp 70–77

  • Coskun S, Girisgin O, Kürkcüoglu M, Malyer H, Girisgin AO, Kırımer N, Baser KH (2008) Acaricidal efficacy of Origanum onites L. essential oil against Rhipicephalus turanicus (Ixodidae). Parasitol Res 103:259–261

    Article  PubMed  Google Scholar 

  • Davies NW (1990) Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicon and carbowax 20 M phases. J Chromatogr 503:1–24

    Article  CAS  Google Scholar 

  • Del Fabro S, Nazzi F (2008) Repellent effect of sweet basil compounds on Ixodes ricinus ticks. Exp Appl Acarol 45:219–228

    Article  Google Scholar 

  • Fernandes FF, Freitas EPS (2007) Acaricidal activity of an oleoresinous extract from Copaifera reticulata (Leguminosae: Caesalpinioideae) against larvae of the southern cattle tick, Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Vet Parasitol 147:150–154

    Article  Google Scholar 

  • Garcia-Fajardo J, Martinez-Sosa M, Estarron-Espinosa M, Vilarem G, De Santos J (1997) Comparative study of the oil and supercritical CO2 extract of Mexican pimento (Pimenta dioica Merrill). J Essent Oil Res 9:181–185

    CAS  Google Scholar 

  • Graham OH, Hourrigan JL (1977) Eradication programs for the arthropod parasites of livestock. J Med Entomol 13:629–658

    CAS  PubMed  Google Scholar 

  • Iacobellis NS, Lo Cantore P, Capasso F, Senatore F (2005) Antibacterial activity of Cuminum cyminum L. and Carum carvi L. essential oils. J Agric Food Chem 53:57–61

    Article  CAS  PubMed  Google Scholar 

  • Jennings W, Shibamoto T (1980) Qualitative analysis of flavor and fragrance volatiles by glass capillary gas chromatography. Appendix III, Retention indices in increasing order on polyethylene glycol carbowax 20 M. Academic, New York, p 472

    Google Scholar 

  • Kéita SK, Vincent C, Schmit JP, Ramaswamy S, Bélanger A (2000) Effect of various essential oils on Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). J Stored Prod Res 36:355–364

    Article  PubMed  Google Scholar 

  • Kéita SM, Vincent C, Schmit JP, Arnason JT, Bélanger A (2001) Efficacy of essential oil of Ocimum basilicum L. and O. gratissimum L. applied as an insecticidal fumigant and powder to control Callosobruchus maculatus (Fab.). J Stored Prod Res 37:339–349

    Article  PubMed  Google Scholar 

  • Kim EH, Kim HK, Ahn YJ (2003) Acaricidal activity of clove bud oil compounds against Dermatophagoides farinae and Dermatophagoides pteronyssinus (Acari: Pyroglyphidae). J Agric Food Chem 51:885–889

    Article  CAS  PubMed  Google Scholar 

  • Kim SI, Yi JH, Tak JH, Ahn YJ (2004) Acaricidal activity of plant essential oils against Dermanyssus gallinae (Acari: Dermanyssidae). Vet Parasitol 120:297–304

    Article  PubMed  Google Scholar 

  • Knio KM, Usta J, Dagher S, Zournajian H, Kreydiyyeh S (2008) Larvicidal activity of essential oils extracted from commonly used herbs in Lebanon against the seaside mosquito, Ochlerotatus caspius. Bioresour Technol 99:763–768

    Article  CAS  PubMed  Google Scholar 

  • Kostic M, Popovic Z, Brkic D, Milanovic S, Sivcev I, Stankovic S (2008) Larvicidal and antifeedant activity of some plant-derived compounds to Lymantria dispar L. (Lepidoptera: Limantriidae). Bioresour Technol 99:7897–7901

    Article  CAS  PubMed  Google Scholar 

  • Marzouk MS, Moharram FA, Mohamed MA, Gamal-Eldeen AM, Aboutabl EA (2007) Anticancer and antioxidant tannins from Pimenta dioica leaves. Z Naturforsch C 62:526–536

    CAS  PubMed  Google Scholar 

  • Miller RJ, Davey RB, George JE (2005) First report of organophosphate-resistant Boophilus microplus (Acari: Ixodidae) within the United States. J Med Entomol 42:912–917

    Article  CAS  PubMed  Google Scholar 

  • Miller RJ, Davey RB, George JE (2007) First report of permethrin-resistant Boophilus microplus (Acari. Ixodidae) collected within the United States. J Med Entomol 44:308–315

    Article  CAS  PubMed  Google Scholar 

  • Miyajima Y, Kikuzaki H, Hisamoto M, Nikatani N (2004) Antioxidative polyphenols from berries of Pimenta dioica. Biofactors 21:301–303

    Article  CAS  PubMed  Google Scholar 

  • Murugan K, Murugan P, Noortheen A (2007) Larvicidal and repellent potential of Albizzia amara Boivin and Ocimum basilicum Linn against dengue vector, Aedes aegypti (Insecta:Diptera:Culicidae). Bioresour Technol 98:198–201

    Article  CAS  PubMed  Google Scholar 

  • Mwangi EN, Hassanali A, Essuman S, Myandat E, Moreka L, Kimondo M (1995) Repellent and acaricidal properties of Ocimum suave against Rhipicephalus appendiculatus ticks. Exp Appl Acarol 19:11–18

    Article  CAS  PubMed  Google Scholar 

  • Nakatani N (2000) Phenolic antioxidants from herbs and spices. Biofactors 13:141–146

    Article  CAS  PubMed  Google Scholar 

  • Nostro A, Cellini L, Di Bartolomeo S, Di Campli E, Grande R, Cannatelli MA, Marzio L, Alonzo V (2005) Antibacterial effect of plant extracts against Helicobacter pylori. Phytother Res 19:198–202

    Article  CAS  PubMed  Google Scholar 

  • Pamo TE, Tendonkeng F, Kana JR, Tenekeu G, Tapondjou LA, Payne VK (2004) The acaricidal effect of the essential oil of Ageratum houstonianum Mill. flowers on ticks (Rhipicephalus lunulatus) in Cameroon. S Afr J Anim Sci 34:244–247

    CAS  Google Scholar 

  • Pamo ET, Tendonkeng F, Kana JR, Payne VK, Boukila B, Lemoufouet J, Miegoue E, Nanda AS (2005) A study of the acaricidal properties of an essential oil extracted from the leaves of Ageratum houstonianum. Vet Parasitol 128:319–323

    Article  Google Scholar 

  • Park IK, Kim J, Lee SG, Shin SC (2007) Nematicidal activity of plant essential oils and components from ajowan (Trachyspermum ammi), allspice (Pimenta dioica) and litsea (Litsea cubeba) essential oils against pine wood nematode (Bursaphelenchus xylophilus). J Nematol 39:275–279

    CAS  PubMed  Google Scholar 

  • Park IK, Kim JN, Lee YS, Lee SG, Ahn YJ, Shin SC (2008) Toxicity of plant essential oils and their components against Lycoriella ingenua (Diptera: Sciaridae). J Econ Entomol 39:275–279

    Google Scholar 

  • Pavela R (2004) Insecticidal activity of certain medicinal plants. Fitoterapia 75:745–749

    Article  PubMed  Google Scholar 

  • Pavela R (2008) Larvicidal effects of various Euro-Asiatic plants against Culex quinquefasciatus Say larvae (Diptera: Culicidae). Parasitol Res 102:555–559

    Article  PubMed  Google Scholar 

  • Pereira JR, Famadas KM (2006) The efficiency of extracts of Dahlstedtia pentaphylla (Leguminosae, Papilionoidae, Millettiedae) on Boophilus microplus (Canestrini, 1887) in artificially infested bovines. Vet Parasitol 142:192–195

    Article  CAS  PubMed  Google Scholar 

  • Prajapati V, Tripathi AK, Aggarwal KK, Khanuja SPS (2005) Insecticidal, repellent and oviposition-deterrent activity of selected essential oils against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. Bioresour Technol 96:1749–1757

    Article  CAS  PubMed  Google Scholar 

  • Ramos A, Visozo A, Piloto J, García A, Rodríguez CA, Rivero R (2003) Screening of antimutagenicity via antioxidant activity in Cuban medicinal plants. J Ethnopharmacol 87:241–246

    Article  CAS  PubMed  Google Scholar 

  • Rao MS, Pratibh G, Korwar GR (2000) Evaluation of aromatic oils against Helicoverpa armigera. Ann Plant Prot Sci 8:236–238

    Google Scholar 

  • Ribeiro VLS, Toigo E, Bordignon AL, Gonçalves K, von Poser G (2007) Acaricidal properties of extracts from the aerial parts of Hypericum polyanthemum on the cattle tick Boophilus microplus. Vet Parasitol 147:199–203

    Article  PubMed  Google Scholar 

  • Rodriguez-Vivas RI, Alonso-Díaz MA, Rodríguez-Arevalo F, Fragoso-Sanchez H, Santamaría VM, Rosario-Cruz R (2006a) Prevalence and potential risk factors for organophosphate and pyrethroid resistance in Boophilus microplus ticks on cattle ranches from the State of Yucatan, Mexico. Vet Parasitol 136:335–342

    Article  CAS  PubMed  Google Scholar 

  • Rodriguez-Vivas RI, Rodríguez-Arevalo F, Alonso-Díaz MA, Fragoso-Sanchez H, Santamaría VM, Rosario-Cruz R (2006b) Prevalence and potential risk factors for amitraz resistance in Boophilus microplus ticks in cattle farms in the State of Yucatan, Mexico. Prev Vet Med 75:280–286

    Article  CAS  PubMed  Google Scholar 

  • Rosario-Cruz R, Guerrero FD, Miller RJ, Rodriguez-Vivas RI, Domínguez-García DI, Cornel AJ, Hernandez-Ortiz R, George JE (2005) Roles played by esterase activity and by a sodium channel mutation involved in pyrethroid resistance in populations of Boophilus microplus (Acari: Ixodidae) collected from Yucatan, Mexico. J Med Entomol 42:1020–1025

    Article  CAS  PubMed  Google Scholar 

  • Seo MI, Kim J, Lee SG, Shin CH, Shin SC, Park IL (2009) Fumigant antitermitic activity of plant essential oils and components from ajowan (Trachyspermum ammi), allspice (Pimenta dioica), caraway (Carum carvi), dill (Anethum graveolens), geranium (Pelargonium graveolens), and litsea (Litsea cubeba) oils against Japanese termite (Reticulitermes speratus Kolbe). J Agric Food Chem 57:6596–6602

    Article  CAS  PubMed  Google Scholar 

  • Singh G, Kapoor IPS, Pandey SK, Singh UK, Singh RK (2002) Studies on essential oils: Part 10; antibacterial activity of volatile oils of some spices. Phytother Res 16:680–682

    Article  CAS  PubMed  Google Scholar 

  • Stone BF, Haydock KP (1962) A method for measuring the acaricide susceptibility of the cattle Boophilus microplus (CAN). Bull Entomol Res 53:563–578

    Article  CAS  Google Scholar 

  • Waliwitiya R, Isman MB, Vernon RS, Riseman A (2005) Insecticidal activity of selected monoterpenoids and rosemary oil to Agriotes obscurus (Coleoptera: Elateridae). J Econ Entomol 98:1560–1565

    Article  CAS  PubMed  Google Scholar 

  • Waliwitiya R, Kennedy CJ, Lowenberger CA (2009) Larvicidal and oviposition-altering activity of monoterpenoids, trans-anithole and rosemary oil to the yellow fever mosquito Aedes aegypti (Diptera: Culicidae). Pest Manag Sci 65:241–248

    Article  CAS  PubMed  Google Scholar 

  • Yang YC, Lee SH, Lee WJ, Choi DH, Ahn YJ (2003) Ovicidal and adulticidal effects of Eugenia caryophyllata bud and leaf oil compounds on Pediculus capitis. J Agric Food Chem 51:4884–4888

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors thank Winston Smith and Maritza Estela Piña Zavala for critical reading of this work.

Ethical standards

All experiments comply with the current laws of Mexico.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Col. Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico

    Moises Martinez-Velazquez, Gustavo Adolfo Castillo-Herrera, Jose Miguel Flores-Fernandez, Julisa Lopez-Ramirez, Rodolfo Hernandez-Gutierrez & Eugenia del Carmen Lugo-Cervantes

  2. Centro Nacional de Investigaciones en Parasitología Veterinaria-INIFAP, No. 8534, Carretera Federal Cuernavaca Cuautla, Colonia Progreso, 62550, Jiutepec, Morelos, Mexico

    Rodrigo Rosario-Cruz

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  1. Moises Martinez-Velazquez
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  2. Gustavo Adolfo Castillo-Herrera
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  3. Rodrigo Rosario-Cruz
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  4. Jose Miguel Flores-Fernandez
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  6. Rodolfo Hernandez-Gutierrez
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  7. Eugenia del Carmen Lugo-Cervantes
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Correspondence to Moises Martinez-Velazquez.

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Martinez-Velazquez, M., Castillo-Herrera, G.A., Rosario-Cruz, R. et al. Acaricidal effect and chemical composition of essential oils extracted from Cuminum cyminum, Pimenta dioica and Ocimum basilicum against the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Parasitol Res 108, 481–487 (2011). https://doi.org/10.1007/s00436-010-2069-6

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Keywords

  • Eugenol
  • Linalool
  • Cumin
  • Methyl Eugenol
  • Acaricidal Activity