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GC-MS analysis and antileishmanial activities of two Turkish propolis types

Abstract

Propolis is a honeybee product with a very complex chemical composition and various pharmacological properties. This study was aimed to investigate antileishmanial activities of “Bursa” and “Hatay” propolis samples against Leishmania infantum and Leishmania tropica strains. Propolis samples were analysed with the gas chromatography-mass spectrometry technique. Promastigotes were incubated in Roswell Park Memorial Institute culture medium in the absence and presence of several concentrations (50, 100, 250, 500, 750, and 1,000 μg/mL) of each propolis sample. The viability and cell morphology of promastigotes in each concentration were examined after 24, 48, 72, and 96 h of incubation. The growth of leishmania parasites was significantly suppressed in the presence of 500, 750, and 1,000 μg/mL of Hatay propolis. Bursa propolis was found to be efficient in inhibiting the growth of leishmania promastigotes in culture media at these concentrations, 250, 500, 750, and 1,000 μg/mL. Thus, the in vitro results showed that the Hatay and Bursa propolis samples decreased significantly the proliferation of L. infantum and L. tropica parasites (p < 0.001); however, Bursa propolis was found to be more effective than Hatay propolis against leishmania promastigotes. These two natural products may be useful agents in the prevention of leishmanial infections.

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References

  • Aksoy A, Duran N, Toroglu S, Koksal F (2007) Short-term effect of mastic gum on salivary concentrations of cariogenic bacteria in orthodontic patients. Angle Orthod 77:124–128

    Article  PubMed  Google Scholar 

  • Allaboutturkey (2009) http://www.allaboutturkey.com/millipark.htm. Accessed 11 Jan 2009

  • Allahverdiyev A, Duran N, Ozguven M, Koltas S (2004) Antiviral activity of the volatile oils of Melissa officinalis L. against herpes simplex virus type-2. Phytomedicine 11:657–661

    CAS  Article  PubMed  Google Scholar 

  • Ayres DC, Marcucci MC, Giorgio S (2007) Effects of Brazilian propolis on Leishmania amazonensis. Mem Inst Oswaldo Cruz 102:215–220

    PubMed  Google Scholar 

  • Bankova V, Popova M, Bogdanov S, Sabatini A (2002) Chemical composition of European propolis: expected and unexcepted results. Z Naturforsch C 57:530–533

    CAS  PubMed  Google Scholar 

  • Benkovic V, Knezevic AH, Orsolic N et al (2009) Evaluation of radioprotective effects of propolis and its flavonoid constituents: in vitro study on human white blood cells. Phytother Res 23:1159–1168

    CAS  Article  PubMed  Google Scholar 

  • Berman J (2003) Current treatment approaches to leishmaniasis. Curr Opin Infect Dis 16:397–401

    CAS  Article  PubMed  Google Scholar 

  • CDC (2009) http://www.cdc.gov/ncidod/dpd/parasites/leishmania/factsht_leishmania.htm#common. Accessed 11 Jan 2009

  • Croft SL, Coombs GH (2003) Leishmaniasis—current chemotherapy and recent advances in the search for novel drugs. Trends Parasitol 19:502–508

    CAS  Article  PubMed  Google Scholar 

  • Cuesta A, Rodriguez A, Esteban MA, Meseguer J (2005) In vivo effects of propolis, a honeybee product, on gilthead seabream innate immune responses. Fish Shellfish Immunol 18:71–80

    CAS  Article  PubMed  Google Scholar 

  • David J, Satoskar AR, Brombacher F, Shoemaker CB, Titus RG, Boza M (1997) Immunomodulatory properties of maxadilan, a peptide derived from sand fly saliva. Acta Parasitol Turcica 21:174

    Google Scholar 

  • Diaz-Carballo D, Malak S, Bardenheuer W, Freistuehler M, Peter Reusch H (2008) The contribution of plukenetione A to the anti-tumoral activity of Cuban propolis. Bioorg Med Chem 16:9635–9643

    CAS  Article  PubMed  Google Scholar 

  • Duran G, Duran N, Culha G, Ozcan B, Oztas H, Ozer B (2008) In vitro antileishmanial activity of Adana propolis samples on Leishmania tropica: a preliminary study. Parasitol Res 102:1217–1225

    CAS  Article  PubMed  Google Scholar 

  • Duran N, Koc A, Oksuz H et al (2006) The protective role of topical propolis on experimental keratitis via nitric oxide levels in rabbits. Mol Cell Biochem 281:153–161

    CAS  Article  PubMed  Google Scholar 

  • Freitas SF, Shinohara L, Sforcin JM, Guimaraes S (2006) In vitro effects of propolis on Giardia duodenalis trophozoites. Phytomedicine 13:170–175

    CAS  Article  PubMed  Google Scholar 

  • Ghisalberti EL (1979) Propolis: a review. Bee World 60:59–84

    CAS  Google Scholar 

  • Kumazawa S, Hamasaka T, Nakayama T (2004) Antioxidant activity of propolis of various geographic origins. Food Chem 84:329–339

    CAS  Article  Google Scholar 

  • Machado GM, Leon LL, De Castro SL (2007) Activity of Brazilian and Bulgarian propolis against different species of Leishmania. Mem Inst Oswaldo Cruz 102:73–77

    Article  PubMed  Google Scholar 

  • Marcucci MC, Ferreres F, Custodio AR et al (2000) Evaluation of phenolic compounds in Brazilian propolis from different geographic regions. Z Naturforsch C 55:76–81

    CAS  PubMed  Google Scholar 

  • McLennan SV, Bonner J, Milne S et al (2008) The antiinflammatory agent propolis improves wound healing in a rodent model of experimental diabetes. Wound Repair Regen 16:706–713

    Article  PubMed  Google Scholar 

  • Murray HW, Berman JD, Davies CR, Saravia NG (2005) Advance in leishmaniasis. Lancet 366:1561–1577

    CAS  Article  PubMed  Google Scholar 

  • Newton SM, Lau C, Gurcha SS, Besra GS, Wright CW (2002) The evaluation of forty-three plant species for in vitro antimycobacterial activities; isolation of active constituents from Psoralea corylifolia and Sanguinaria canadensis. J Ethnopharmacol 79:57–67

    Article  PubMed  Google Scholar 

  • Ok UZ, Balcioglu IC, Ozkan AT, Ozensoy S, Ozbel Y (2002) Leishmaniasis in Turkey. Acta Trop 84: 43–48

    CAS  Article  PubMed  Google Scholar 

  • Oksuz H, Duran N, Tamer C, Cetin M, Silici S (2005) Effect of propolis in the treatment of experimental Staphylococcus aureus keratitis in rabbits. Ophthalmic Res 37:328–334

    CAS  Article  PubMed  Google Scholar 

  • Onlen Y, Duran N, Atik E et al (2007a) Antibacterial activity of propolis against MRSA and synergism with topical mupirocin. J Altern Complement Med 13:713–718

    Article  PubMed  Google Scholar 

  • Onlen Y, Tamer C, Oksuz H, Duran N, Altug ME, Yakan S (2007b) Comparative trial of different anti-bacterial combinations with propolis and ciprofloxacin on Pseudomonas keratitis in rabbits. Microbiol Res 162:62–68

    CAS  Article  PubMed  Google Scholar 

  • Pontin K, Da Silva Filho AA, Santos FF et al (2008) In vitro and in vivo antileishmanial activities of a Brazilian green propolis extract. Parasitol Res 103:487–492

    Article  PubMed  Google Scholar 

  • Prytzyk E, Dantas AP, Salomao K et al (2003) Flavonoids and trypanocidal activity of Bulgarian propolis. J Ethnopharmacol 88:189–193

    CAS  Article  PubMed  Google Scholar 

  • Salatino A, Teixeira EW, Negri G, Message D (2005) Origin and chemical variation of Brazilian propolis. Evid Based Complement Alternat Med 2:33–38

    Article  PubMed  Google Scholar 

  • Salomao K, Pereira PR, Campos LC et al (2008) Brazilian propolis: correlation between chemical composition and antimicrobial activity. Evid Based Complement Alternat Med 5:317–324

    Article  PubMed  Google Scholar 

  • Shimizu T, Hino A, Tsutsumi A, Park YK, Watanabe W, Kurokawa M (2008) Anti-influenza virus activity of propolis in vitro and its efficacy against influenza infection in mice. Antivir Chem Chemother 19:7–13

    CAS  PubMed  Google Scholar 

  • Siqueira AB, Gomes BS, Cambuim I et al (2009) Trichophyton species susceptibility to green and red propolis from Brazil. Lett Appl Microbiol 48:90–96

    CAS  Article  PubMed  Google Scholar 

  • Sorkun K, Suer B, Salih B (2001) Determination of chemical composition of Turkish propolis. Z Naturforsch C 56:666–668

    CAS  PubMed  Google Scholar 

  • Teixeira EW, Message D, Negri G, Salatino A, Stringheta PC (2008) Seasonal variation, chemical composition and antioxidant activity of Brazilian propolis samples. Evid Based Complementary Alternat Med (in press)

  • Uzel A, Sorkun K, Oncag O, Cogulu D, Gencay O, Salih B (2005) Chemical compositions and antimicrobial activities of four different Anatolian propolis samples. Microbiology Res 160:189–195

    CAS  Article  Google Scholar 

  • WHO (2009) http://www.who.int/leishmaniasis/burden/en/. Accessed 11 Jan 2009

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Correspondence to Nizami Duran.

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Duran, N., Muz, M., Culha, G. et al. GC-MS analysis and antileishmanial activities of two Turkish propolis types. Parasitol Res 108, 95–105 (2011). https://doi.org/10.1007/s00436-010-2039-z

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  • DOI: https://doi.org/10.1007/s00436-010-2039-z

Keywords

  • Visceral Leishmaniasis
  • Leishmaniasis
  • Propolis
  • Cutaneous Leishmaniasis
  • Leishmania Parasite