Abstract
Chrysosporium keratinophilum is known to be a keratinophilic fungus and an effective mosquito control agent. This fungus was grown on Sabauraud dextrose broth in the laboratory at 25°C, while the relative humidity was maintained at 75 ± 5% for 15 days. Filtration process of metabolites was done using whatman-1 filter paper, column chromatography and flash, chromatography. Larvicidal efficacy was performed against all instars of Culex quinquefasciatus. Larvicidal efficacy was performed at six different concentrations with different effective ratios (ethanol/metabolites: 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, and 1:9). The mortality values were then subjected by the probit analysis. The larval mortalities were observed for a period of 24, 48, and 72 h, respectively. The first and second instars were highly susceptible to 2:8 ratio. In the first instar after column chromatography, LC50 = 26.66 ppm, LC90 = 121.96 ppm, LC99 = 231.86 ppm were observed after 72 h, while after flash chromatography the LC50 = 20 ppm, LC90 = 123.02 ppm, LC99 = 281.83 ppm were observed after 48 h. In the second instar after column chromatography, LC50 = 18.19 ppm, LC90 = 102.32 ppm, LC99 = 162.18 ppm were observed after 72 h, while doing flash chromatography 100% mortality could be recorded after 24 h. In the third instar after column chromatography, the LC50 = 38.01 ppm, LC90 = 131.82 ppm, LC99 = 245.47 ppm were observed after 72 h, while after flash chromatography the LC50 = 17.78 ppm, LC90 = 100 ppm, LC99 = 151.35 ppm. In the fourth instar, LC50 = 61.65 ppm, LC90 = 181.97 ppm, LC99 = 436.51 ppm, while after flash chromatography LC50 = 40 ppm, LC90 = 120 ppm, and LC99 = 223.87 ppm were observed after 72 h. The extracellular metabolites of C. keratinophilum could be a fungal based larvicides resource for the control of C. quinquefasciatus larvae. This could be another agent for biotechnological exploitation, if found suitable in field trials.
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Acknowledgments
We thank Prof. V.G. Das, Director, Dayalbagh Educational Institute, for his encouragements. We are also thankful to the Department of Science and Technology for the financial support (DST, C-24/Soam Prakash) to conduct the research and to DST-FIST program (2003–2008) for providing laboratory facilities.
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Soni, N., Prakash, S. Effect of Chrysosporium keratinophilum metabolites against Culex quinquefasciatus after chromatographic purification. Parasitol Res 107, 1329–1336 (2010). https://doi.org/10.1007/s00436-010-2003-y
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DOI: https://doi.org/10.1007/s00436-010-2003-y