Essential oil of Plectranthus amboinicus was studied for its chemical composition and larvicidal potential against the malarial vector mosquito Anopheles stephensi. Totally 26 compounds were identified by GC and GC-MS. The major chemical compounds were carvacrol (28.65%) followed by thymol (21.66%), α-humulene (9.67%), undecanal (8.29%), γ-terpinene (7.76%), ρ-cymene (6.46%), caryophyllene oxide (5.85%), α-terpineol (3.28%) and β-selinene (2.01%). The larvicidal assay was conducted to record the LC50 and LC90 values and the larval mortality was observed after 12 and 24 h of exposure period. The LC50 values of the oil were 33.54 (after 12 h) and 28.37 ppm (after 24 h). The LC90 values of the oil were 70.27 (after 12 h) and 59.38 ppm (after 24 h). The results of the present study showed that the essential oil of P. amboinicus is one of the inexpensive and eco-friendly sources of natural mosquito larvicidal agent to control/reduce the population of malarial vector mosquito.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Adams RP (2009) Identification of essential oil compounds by gas chromatography and mass spectrometry, 4th edn. Allured Publishing Corporation, Corol stream
Amer A, Mehlhorn H (2006) Larvicidal effects of varies essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitol Res 99:466–472. doi:10.1007/s00436-006-0182-3
Baser KHC, Ozek T, Tümen G, Sezik G (1993) Composition of the essential oil of Turkish Origanum species with commercial importance. J Essent Oil Res 5:619–623
Bassole IH, Guelbeogo WM, Nebie R, Costantini C, Sagnon N, Kabore ZI, Traore SA (2004) Ovicidal and larvicidal activity against Aedes aegypti and Anopheles gambiae complex mosquitoes of essential oils extracted from three spontaneous plants of Burkina Faso. Parasitologia 45:23–26
Carvalho AFU, Melo VMM, Craveiro AA, Machado MIL, Bantim MB, Rabelo EF (2003) Larvicidal activity of the essential oil from Lippia sidoides Cham against Aedes aegypti L. Mem Inst Oswaldo Cruz 98:569–571
Cooper RA, Hartwig CL, Ferdig MT (2005) pfcrt is moe than the Plasmodium falciparum chloroquine resistance gene: a functional and evolutionary perspective. Acta Trop 94:170–180. doi:10.1016/j.actatropica.2005.04.004
Garcia LS (2010) Malaria. Clin Lab Med 30:93–129. doi:10.1016/j.cll.2009.10.001
Githeko (2008) Malaria, climate change and possible impacts on populations in Africa. In: Caraël M, Glynn JR (eds) HIV, resurgent infections and population change in Africa. Springer Science, The Netherlands, pp 67–77
Githeko AK, Lindsay SW, Confalonieri U, Partz J (2000) Climate changes and vector borne diseases: a regional analysis. Bull WHO 78:1136–1147
Knio KM, Usta J, Dagher S, Zournajiam H, Kreydiyyeh S (2008) Larvicidal activity of essential oil extracted from commonly used herbs in Lebanon against the seaside mosquito, Ochlerotatus caspius. Bioresour Technol 99:763–768. doi:10.1016/j.biortech.2007.01.026
Lal S, Laharia C, Saxena VK (2010) Insecticide treated nets, antimalarials and child survival in India. Indian J Pediatr 77:425–430. doi:10.1007/s12098-010-0051-z
Moretti MDL, Sanna-Passino G, Demontis S, Bazzoni E (2002) Essential oil formulations useful as a new tool for insect pest control. AAPS PharmSciTech 3:1–11
Murthy PS, Ramalakshmi K, Srinivas P (2009) Fungitoxic activity of Indian borage (Plectranthus amboinicus) volatiles. Food Chem 114:1014–1018. doi:10.1016/j.foodchem.2008.10.064
Raghavendra K, Subbarao SK (2002) Chemical insecticides in malaria vector control in India. ICMR Bull 32:1–7
Senthilkumar N, Verma P, Gurusubamanian G (2009) Larvicidal and adulticidal activities of some medicinal plants against the malarial vector Anopheles stephensi (Liston). Parasitol Res 104:237–244. doi:10.1007/s00436-008-1180-4
Traboulsi AF, Taoubi K, El-Haj S, Bessiere JM, Rammal S (2002) Insecticidal properties of essential plant oils against the mosquito Culex pipiens molestus (Diptera: Culicidae). Pest Manage Sci 58:491–495. doi:10.1002/ps.486
World Health Organisation (1981) Instruction for determining the susceptibility or resistance of mosquito larvae to insecticides. WHO-VBC 81.807, pp 1–6
World Health Organization (1989) Geographical distribution of arthropod-borne diseases and their principal vectors. WHO/VBC89.967
The authors are grateful to Dr. R. Panneerselvam, Professor and Head, Department of Botany, Annamalai University, for providing laboratory facilities for the study. We are thankful to The Director, Centre for Research in Medical Entomology (ICMR-Government of India), Madurai for mosquito egg supply.
About this article
Cite this article
Senthilkumar, A., Venkatesalu, V. Chemical composition and larvicidal activity of the essential oil of Plectranthus amboinicus (Lour.) Spreng against Anopheles stephensi: a malarial vector mosquito. Parasitol Res 107, 1275–1278 (2010). https://doi.org/10.1007/s00436-010-1996-6
- Larvicidal Activity
- Anopheles Stephensi