Abstract
The MYND-type zinc finger protein (MYND-ZF) is a large group of proteins containing the MYND domain which play an important role in protein–protein interactions. A cDNA clone encoding a novel MYND-ZF was isolated and identified from a Clonorchis sinensis (C. sinensis) adult cDNA library. The open reading frame of this novel cDNA sequence contains 1,440 base pairs with a putative protein of 479 amino acids showing a high homology with the MYND-ZF identified from other species. Recombinant CsMYND-ZF was expressed and purified from Escherichia coli BL21 (DE3). CsMYND-ZF transcripts were detected in the cDNA of adult worms and metacercariae but not in eggs of C. sinensis. Immunohistochemistry results revealed that CsMYND-ZF was deposited at the tegument of adult worms and metacercariae C. sinensis using anti-recombinant CsMYND-ZF serum. These findings may contribute to the development of a reliable diagnostic method.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abu-Farha M, Lambert JP, Al-Madhoun AS, Elisma F, Skerjanc IS, Figeys D (2008) The tale of two domains: proteomics and genomics analysis of SMYD2, a new histone methyltransferase. Mol Cell Proteomics 7(3):560–572
Ansieau S, Leutz A (2002) The conserved Mynd domain of BS69 binds cellular and oncoviral proteins through a common PXLXP motif. J Biol Chem 277(7):4906–4910
Black AR, Black JD, Azizkhan-Clifford J (2001) Sp1 and krüppel-like factor family of transcripttion factors in cell growth regulation and cancer. J Cell Physiol 188(2):143–160
Chung CS, Lee SK (1976) An epidemiological study of primary liver carcinomas in Busan area with special reference to clonorchiasis. Korean J Pathol 10(3):33–46
Gross CT, McGinnis W (1996) DEAF-1, a novel protein that binds an essential region in a deformed response element. EMBO J 15(8):1961–1970
Hashimoto M, Murata E, Aokil T (2010) Secretory protein with RING finger domain (SPRING) specific to Trypanosoma cruzi is directed, as a ubiquitin ligase related protein, to the nucleus of host cells. Cell Microbiol 12(1):19–30
Hoovers JM, Mannens M, John R, Bliek J, van Heyningen V, Porteous DJ, Leschot NJ, Westerveld A, Little PF (1992) High-resolution localization of 69 potential human zinc finger protein genes: a number are clustered. Genomics 12(2):254–263
Jeffs SA, Hagan P, Allen R, Correa-Oliveira R, Smithers SR, Simpson AJ (1991) Molecular cloning and characterisation of the 22-kilodalton adult Schistosoma mansoni antigen recognized by antibodies from mice protectively vaccinated with isolated tegumental surface membranes. Mol Biochem Parasitol 46(1):159–167
Jones MK, Gobert GN, Zhang L, Sunderland P, McManus DP (2004) The cytoskeleton and motor proteins of human schistosomes and their roles in surface maintenance and host-parasite interactions. Bioessays 26(7):752–765
Komatsu S, Imoto I, Tsuda H, Kozaki KI, Muramatsu T, Shimada Y, Aiko S, Yoshizumi Y, Ichikawa D, Otsuji E, Inazawa J (2009) Overexpression of SMYD2 relates to tumor cell proliferation and malignant outcome of esophageal squamous cell carcinoma. Carcinogenesis 30(7):1139–1146
Lee JH, Yang HM, Bak UB, Rim HJ (1994) Promoting role of Clonorchis sinensis infection on induction of cholangiocarcinoma during two-step carcinogenesis. Korean J Parasitol 32(1):13–18
Lee JA, Suh DC, Kang JE, Kim MH, Park H, Lee MN, Kim JM, Jeon BN, Roh HE, Yu MY, Choi KY, Kim KY, Hur MW (2005) Transcriptional activity of Sp1 is regulated by molecular interactions between the zinc finger DNA binding domain and the inhibitory domain with corepressors, and this interaction is modulated by MEK. J Biol Chem 280(30):28061–28071
Li L, He S, Sun JM, Davie JR (2004) Gene regulation by Sp1 and Sp3. Biochem Cell Biol 82(4):460–471
Lun ZR, Gasser RB, Lai DH, Li AX, Zhu XQ, Yu XB, Fang YY (2005) Clonorchiasis: a key foodborne zoonosis in China. Lancet Infect Dis 5(1):31–41
Lutterbach B, Westendorf JJ, Linggi B, Patten A, Moniwa M, Davie JR, Huynh KD, Bardwell VJ, Lavinsky RM, Rosenfeld MG, Glass C, Seto E, Hiebert SW (1998) ETO, a target of t(8;21) in acute leukemia, interacts with the N-CoR and mSin3 corepressors. Mol Cell Biol 18(12):7176–7184
Miller J, McLachlan AD, Klug A (1985) Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. EMBO J 4(6):1609–1614
Rim HJ (2005) Clonorchiasis: an update. J Helminthol 79(3):269–281
Scarr RB, Sharp PA (2002) PDCD2 is a negative regulator of HCF-1 (C1). Oncogene 21(34):5245–5254
Song LX, Chen SY, Yu XB, Wu ZD, Xu J, Yang G, Zheng NC, Hu XC, Guo LC, Dai JF, Xu J, Ji CN, Gu SH, Ying K (2004) Molecular cloning and characterization of cDNA encoding a ubiquitinconjugating enzyme from Clonorchis sinensis. Parasitol Res 94(3):227–232
Spadaccini R, Perrin H, Bottomley MJ, Ansieau S, Sattler M (2006) Structure and functional analysis of the MYND domain. J Mol Biol 358(2):498–508
Van Hellemond JJ, Retra K, Brouwers JFHM, Balkom BWM, Yazdanbakhsh M, Shoemaker CB, Tielens AGM (2006) Functions of the tegument of schistosomes: clues from the proteome and lipidome. Int J Parasitol 36(6):691–699
Waine GJ, Becker MM, Scott JC, Kalinna BH, Yang W, McManus DP (1994) Purification of a recombinant Schistosoma japonicum antigen homologous to the 22-kDa membrane-associated antigen of S. mansoni, a putative vaccine candidate against schistosomiasis. Gene 142(2):259–263
Wang J, Hoshino T, Redner RL, Kajigaya S, Liu JM (1998) ETO, fusion partner in t(8;21) acute myeloid leukemia, represses transcription by interaction with the human N-CoR/mSin3/HDAC1 complex. Proc Natl Acad Sci USA 95(18):10860–10865
Zeng W, Kong Q, Li C, Mao B (2010) Xenopus RCOR2 (REST corepressor 2) interacts with ZMYND8, which is involved in neural differentiation. Biochem Biophys Res Commun 394(4):1024–9
Zheng NC, Xu J, Wu ZD, Chen JZ, Hu XC, Song LX, Yang G, Ji CN, Chen SY, Gu SH, Ying K, Yu XB (2005) Clonorchis sinensis: molecular cloning and functional expression of novel cytosolic malate dehydrogenase. Exp Parasitol 109(4):220–227
Acknowledgments
This study was supported by the Natural Science Foundation of Guangdong province (815100891000005), the Medical Research Foundation of Guangdong province (B2008157), and the National Basic Research Program of China (973 program; no. 2010CB530000).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, L., Yu, X., Yang, Y. et al. Molecular characterization and expression of the MYND-ZF gene from Clonorchis sinensis . Parasitol Res 107, 1249–1255 (2010). https://doi.org/10.1007/s00436-010-1995-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-010-1995-7