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Differentiation of Myxobolus spp. (Myxozoa: Myxobolidae) infecting roach (Rutilus rutilus) in Hungary

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Abstract

During a survey on fishes from Hungarian freshwaters, the occurrence of eight Myxobolus species was registered in roach (Rutilus rutilus L.). Most species had a specific location within the fish host. Of the known species infecting roach, the intramuscularly developing species Myxobolus pseudodispar was found to be the most common. Besides Myxobolus intimus and Myxobolus diversicapsularis infecting the capillary network of gill lamellae, Myxobolus feisti infecting the cartilaginous gill rays were observed. Of the “muelleri-type” Myxobolus spp, Myxobolus rutili and Myxobolus sommervillae sp. n. formed elongated plasmodia in the gill filaments outside and inside the arteria efferens, respectively, while Myxobolus wootteni sp. n. developed cysts in fins. Plasmodia and spores of Myxobolus fundamentalis sp. n. were detected in the cartilaginous gill arch under the basis of gill filaments. Despite similarities of some species in spore morphology, 18S rDNA sequences and phylogenetic analyses showed clear differences among all species examined. The findings of the study demonstrate that morphologically similar spores could only be correctly identified by considering the location of plasmodia and the genetic characters of the myxozoan species.

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References

  • Abdel-Ghaffar F, Toukhy A, Al-Quarashi S, Al-Rasheid KAS, Abdel-Baki AS, Hegazy A, Bashtar A-R (2008) Five new myxosporean species (Myxozoa: Myxosporea) infecting the Nile Tilapia) Oreochromis niloticus in Bahr Shebin, Nile Tributary, Nile Delta, Egypt. Parasitol Res 103:1197–1205

    Article  CAS  PubMed  Google Scholar 

  • Ali MA, Abdel-Baki AS, Sakran T, Entzeroth R, Abdel-Ghaffar F (2007) Myxobolus lubati n. sp. (Myxosporea: Myxobolidae), a new parasite of haffara seabream Rhabdosargus haffara (Forsskal, 1775), Red Sea, Egypt: a light and electron transmission microscopy. Parasitol Res 100:819–827

    Article  CAS  PubMed  Google Scholar 

  • Alvarez-Pellitero P, Molnár K, Sitja-Bobadilla A, Cs S (2002) Comparative ultrastructure of the actinosporean stages of Myxobolus bramae and Myxobolus pseudodispar (Myxosporea). Parasitol Res 88:198–207

    Article  CAS  PubMed  Google Scholar 

  • Andree KB, Székely C, Molnár K, Gresoviac SJ, Hedrick RP (1999) Relationships among members of the genus Myxobolus (Myxozoa: Bivalvidae) based on small subunit ribosomal RNA sequences. J Parasitol 85:68–74

    Article  CAS  PubMed  Google Scholar 

  • Baska F (1986) Histological studies on the development of Myxobolus pseudodispar Gorbunova, 1936 in the roach (Rutilus rutilus). Acta Vet Hung 35:251–257

    Google Scholar 

  • Baska F, Voronin VN, Eszterbauer E, Müller L, Sz M, Molnár K (2009) Occurrence of two myxosporean species, Myxobolus hakyi sp. n. and Hoferellus pulvinatus sp. n., in Pangasianodon hypophthalmus fry imported from Thailand to Europe as ornamental fish. Parasitol Res 105:1391–1398

    Article  PubMed  Google Scholar 

  • Cone D, Marcogliese DJ, Russel R (2004) The myxozoan fauna of the spottail shiner in the Great Lakes basin: membership, richness, and geographical distribution. J Parasitol 90:921–932

    Article  PubMed  Google Scholar 

  • Corpet F (1988) Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 16:10881–10890

    Article  CAS  PubMed  Google Scholar 

  • Donec ZS, Shulman SS (1984) Knidosporidii (Cnidosporidia). In: Bauer ON (ed) Key to the determination of parasites of freshwater fishes of the USSR Vol. 1. Nauka, Leningrad, pp 88–251, in Russian

    Google Scholar 

  • Eiras JC, Molnár K, Lu YS (2005) Synopsis of the genus Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea: Myxobolidae). Syst Parasitol 61:1–46

    Article  CAS  PubMed  Google Scholar 

  • Eiras JC, Monteiro CM, Brasil-Sato MC (2010) Myxobolus franciscoi (Myxozoa: Myxosporea: Myxobolidae), a parasite of Prochilodus argenteus (Actinopterygii: Prochilodontidae) from the Upper Sao Francisco River, Brazil, with a revision of Myxobolus spp. from South America. Zoologia 27:131–137

    Google Scholar 

  • Eszterbauer E (2002) Molecular biology can differentiate morphologically indistinguishable myxosporean species: Myxobolus elegans and M. hungaricus. Acta Vet Hung 50:59–62

    Article  PubMed  Google Scholar 

  • Eszterbauer E (2004) Genetic relationship among gill-infecting Myxobolus species (Myxosporea) of cyprinids: molecular evidence of importance of tissue-specificity. Dis Aquat Org 58:35–40

    Article  PubMed  Google Scholar 

  • Eszterbauer E, Székely C (2004) Molecular phylogeny of the kidney-parasitic Sphaerospora renicola from common carp (Cyprinus carpio) and Sphaerospora sp. from goldfish (Carassius auratus auratus). Acta Vet Hung 52:469–478

    Article  CAS  PubMed  Google Scholar 

  • Eszterbauer E, Marton S, Rácz OZ, Letenyei M, Molnár K (2006) Morphological and genetic differences among actinosporean stages of fish-parasitic myxosporeans (Myxozoa): difficulties of species identification. Syst Parasitol 65:97–114

    Article  PubMed  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hallett SL, Diamant A (2001) Ultrastructure and small-subunit ribosomal DNA sequence of Henneguya lesteri n. sp. (Myxosporea), a parasite of sand whiting Sillago analis (Sillaginidae) from the coast of Queensland, Australia. Dis Aquat Org 46:197–212

    Article  CAS  PubMed  Google Scholar 

  • Hallett SL, Atkinson SD, Erséus C, El-Matbouli M (2005) Dissemination of triactinomyxons (Myxozoa) via oligochaetes used as live food for aquarium fishes. Dis Aquat Org 65:137–152

    Article  PubMed  Google Scholar 

  • Hallett SL, Atkinson SD, Erséus C, El-Matbouli M (2006) Myxozoan parasites disseminated via oligochaete worms as live food for aquarium fishes: descriptions of aurantiactinomyxon and raabeia actinospore types. Dis Aquat Org 69:213–225

    Article  PubMed  Google Scholar 

  • Hillis DM, Dixon T (1991) Ribosomal DNA: molecular evolution and phylogenetic inference. Quart Rev Biol 66:411–453

    Article  CAS  PubMed  Google Scholar 

  • Kaur H, Singh R (2010) One new myxosporidian species, Myxobolus slendrii sp. nov., and one known species, M. punjabensis Gupta and Khera, 1989, infecting freshwater fishes in wetlands of Punjab, India. Parasitol Res 106:1043–1047

    Article  PubMed  Google Scholar 

  • Landsberg JJ, Lom J (1991) Taxonomy of the genera of the Myxobolus/Myxosoma group (Myxobolidae: Myxosporea), current listing of species and revision of synonyms. Syst Parasitol 18:165–186

    Article  Google Scholar 

  • Lom J, Dyková I (1992) Protozoan parasites of fishes, volume 26 (Developments in aquaculture and fisheries science). Elsevier, Amsterdam, p 315

    Google Scholar 

  • Longshaw M, Frear P, Feist SW (2003) Myxobolus buckei sp n. (Myxozoa), a new pathogenic parasite from the spinal column of three cyprinid fishes from the United Kingdom. Folia Parasitol 50:251–262

    PubMed  Google Scholar 

  • Molnár K (1994) Comments on the host, organ and tissue specificity of fish myxosporeans and on the types of their intrapiscine development. Parasitol Hung 27:5–20

    Google Scholar 

  • Molnár K, Eszterbauer E, Székely C, Dán Á, Harrach B (2002) Morphological and molecular biological studies on intramuscular Myxobolus spp. of cyprinid fish. J Fish Dis 25:643–652

    Article  Google Scholar 

  • Molnár K, Marton S, Eszterbauer E, Székely C (2006) Comparative morphological and molecular studies on Myxobolus spp. infecting chub from the River Danube, Hungary, and description of M. muellericus sp. n. Dis Aquat Org 73:49–61

    Article  PubMed  Google Scholar 

  • Molnár K, Marton S, Eszterbauer E, Székely C (2007) Description of Myxobolus gayerae n. sp. and redescription of Myxobolus leuciscine infecting the European chub from the Hungarian stretch of the river Danube. Dis Aquat Org 78:147–1543

    Article  PubMed  Google Scholar 

  • Molnár K, Cech G, Székely C (2008) Myxobolus species infecting the cartilaginous rays of the gill filaments in cyprinid fishes. Acta Parasitol 53:330–338

    Article  Google Scholar 

  • Posada D, Crandall KA (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14:817–818

    Article  CAS  PubMed  Google Scholar 

  • ácz OZ, Székely C, Molnár K (2004) Intraoligochaete development of Myxobolus intimus (Myxosporea: Myxobolidae), a gill myxosporean of the roach (Rutilus rutilus). Folia Parasitol 51:199–207

    PubMed  Google Scholar 

  • Reuss H (1906) Neue Myxosporidien von Süsswasserfischen. Bull Acad Imp Sci St-Petersbg Ser 5:199–205

    Google Scholar 

  • Ronquist F, Huelsenbeck JP (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574

    Article  CAS  PubMed  Google Scholar 

  • Shulman SS (1966) Myxosporidia of the fauna of the USSR. Nauka, Moscow, p 504, in Russian

    Google Scholar 

  • Staden R (1996) The Staden sequence analysis package. Mol Biotechnol 5:233–241

    Article  CAS  PubMed  Google Scholar 

  • Swofford DL (2001) PAUP*: phylogenetic analysis using parsimony (*and other methods).Version 4.0b8. Sinauer Associates, Sunderland

    Google Scholar 

  • Székely C, Molnár K, Eszterbauer E, Baska F (1999) Experimental detection of the actinospores of Myxobolus pseudodispar (Myxosporea: Myxobolidae) in oligochaete alternate host. Dis Aquat Org 38:219–224

    Article  PubMed  Google Scholar 

  • Székely C, Molnár K, Rácz O (2001) Complete developmental cycle of Myxobolus pseudodispar (Gorbunova) (Myxosporea: Myxobolidae). J Fish Dis 24:461–468

    Article  Google Scholar 

  • Whipps CM, Adlard RD, Bryant MS, Lester RJ, Findlay V, Kent ML (2003) First report of three Kudoa species from eastern Australia: Kudoa thyrsites from Mahi mahi (Coryphaena hippurus), Kudoa amamiensis and Kudoa minithyrsites n. sp. from sweeper (Pempheris ypsilychnus). J Eukaryot Microbiol 50:215–219

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

The study was supported by the Hungarian Scientific Research Fund (OTKA, projects no. T45891, K75873 and K71837). The authors thank Ms. Györgyi Pataki for preparing drawings and histological slides.

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Authors and Affiliations

  1. Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, 1581, Budapest, Hungary

    Kálmán Molnár, Szilvia Marton, Csaba Székely & Edit Eszterbauer

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  1. Kálmán Molnár
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  2. Szilvia Marton
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  3. Csaba Székely
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  4. Edit Eszterbauer
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Correspondence to Kálmán Molnár.

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Molnár, K., Marton, S., Székely, C. et al. Differentiation of Myxobolus spp. (Myxozoa: Myxobolidae) infecting roach (Rutilus rutilus) in Hungary. Parasitol Res 107, 1137–1150 (2010). https://doi.org/10.1007/s00436-010-1982-z

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