Abstract
After an intraperitoneal infection of mice with Taenia crassiceps metacestodes, peritoneal inflammatory cells labeled with fluoresceinated MoAb anti-mouse were analyzed by flow cytometry. Apoptosis was studied by annexin A/PI, TUNEL assays, DNA laddering, caspase-3 activity, and electron microscopy. An important continuous decrease of CD4+, CD8+ and CD19+ lymphocytes, and an increase of eosinophils and macrophages throughout the observation time were found. Apoptosis of eosinophils was quantified during the observation period with a peak at 6 days post-infection (67.27%). In an additional experiment at 12 days post-infection using TUNEL staining, a high level of apoptosis of eosinophil (92.3%) and a significant decrease of CD4+, CD8+, and CD19+ lymphocytes were confirmed. Caspase-3 activity in peritoneal fluid, peritoneal cells' DNA fragmentation, and apoptosis of eosinophils and monocytes were found. The dramatic decrease of peritoneal inflammatory T and B cells and the high level of apoptosis of inflammatory eosinophils induced in mice by infection with T. crassiceps cysticerci may be important factors of the immunosuppression observed in cysticercosis.
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Acknowledgments
This work was supported by Universidad Nacional Autónoma de México.
We are grateful to Dr. Rodolfo Paredes and Juan Manuel Barbosa for the electron microscopy and computational support. We also thank America Inurreta and Ariadna Molinari for revising the manuscript.
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Zepeda, N., Solano, S., Copitin, N. et al. Decrease of peritoneal inflammatory CD4+, CD8+, CD19+ lymphocytes and apoptosis of eosinophils in a murine Taenia crassiceps infection. Parasitol Res 107, 1129–1135 (2010). https://doi.org/10.1007/s00436-010-1980-1
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DOI: https://doi.org/10.1007/s00436-010-1980-1