Abstract
Echinococcosis/hydatidosis is one of the most important zoonotic diseases commonly found in different regions of Iran with a major economic and public health importance. In the current study, Echinococcus granulosus isolates were collected from hospitalized patients in Isfahan, central Iran. The genotypes of 30 samples were determined by polymerase chain reaction amplification of internal transcribed spacer-1 region of ribosomal DNA, followed by restriction fragment length polymorphism (RFLP) with two restriction enzymes namely AluI and MspI. As expected, each isolate yielded an approximately 1-kbp DNA fragment on the electrophoresis gel. According to RFLP results for both enzymes, all isolates had an equal pattern indicating the G1 genotype. Our findings confirmed that G1 is the dominant genotype of cystic echinococcosis in human in central Iran, with predilection to different organs including liver, lung, and brain, and warrants the importance of sheep dog cycle in public health.
References
Ahmadi N, Dalimi A (2006) Characterization of Echinococcus granulosus isolates from human, sheep and camel in Iran. Infect Genet Evol 6:85–90
Bowles J, McManus DP (1993) Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction-based RFLP method. Mol Biochem Parasitol 57:231–239
Harandi MF, Hobbs RP, Adams PJ, Mobedi I, Morgan-Ryan UM, Thompson RCA (2002) Molecular and morphological characterization of Echinococcus granulosus of human and animal origin in Iran. Parasitol 125:367–373
Jamali R, Ghazanchaei A, Asgharzadeh M (2004) Identification and characterization of Echinococcus granulosus by PCR-RFLP technique in Tabriz district. Journal of Parasitic Diseases 28:69–72
Karimi A, Dianatpour R (2008) Genotypic and phenotypic charactrization of Echinococcus granulosus of Iran. Biotechnology 7:757–762
Lavikainen A, Lehtinen MJ, Meri T, Hirvela-Koski V, Meri S (2003) Molecular genetic characterization of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus. Parasitol 127:207–215
McManus DP, Thompson RC (2003) Molecular epidemiology of cystic echinococcosis. Parasitol 127(Suppl):S37–S51
Moks E, Jogisalu I, Valdmann H, Saarma U (2008) First report of Echinococcus granulosus G8 in Eurasia and a reappraisal of the phylogenetic relationships of ‘genotypes’ G5-G10. Parasitol 135:647–654
Moro PL, Nakao M, Ito A, Schantz PM, Cavero C, Cabrera L (2009) Molecular identification of Echinococcus isolates from Peru. Parasitol Int 58:184–186
Rahimi HR, Kia EB, Mirhendi SH, Talebi A, Harandi MF, Jalali-Zand N, Rokni MB (2007) A new primer pair in ITS1 region for molecular studies on Echinococcus granulosus. Iranian J Publ Health 36:45–49
Rokni M (2009) Echinococcosis/hydatidosis in Iran. Iranian J Parasitol 4:1–16
Saarma U, Jogisalu I, Moks E, Varcasia A, Lavikainen A, Oksanen A, Simsek S, Andresiuk V, Denegri G, Gonzalez LM et al (2009) A novel phylogeny for the genus Echinococcus, based on nuclear data, challenges relationships based on mitochondrial evidence. Parasitol 136:317–328
Sadjjadi S (2006) Present situation of echinococcosis in the Middle East and Arabic North Africa. Parasitol Int 55(Suppl):S197–S202
Scott JC, Stefaniak J, Pawlowski ZS, McManus DP (1997) Molecular genetic analysis of human cystic hydatid cases from Poland: identification of a new genotypic group (G9) of Echinococcus granulosus. Parasitol 114:37–43
Sharbatkhori M, Kia E, Fasihi Harandi M, Jalalizand N, Zahabiun F, Mirhendi H (2009a) Comparison of five simple methods for DNA extraction from Echinococcus granulosus protoscoleces for PCR amplification of ribosomal DNA. Iranian J Parasitol 4:54–60
Sharbatkhori M, Mirhendi H, Jex AR, Pangasa A, Campbell BE, Kia E, Eshraghian M, Fasihi Harandi M, Gasser RB (2009b) Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning-phylogenetic approach. Electrophoresis 30:2648–2655
Sharbatkhori M, Mirhendi H, Harandi MF, Rezaeian M, Mohebali M, Eshraghian M, Rahimi H, Kia EB (2010) Echinococcus granulosus genotypes in livestock of Iran indicating high frequency of G1 genotype in camels. Exp Parasitol 124:373–379
Thompson RCA (2008) The taxonomy, phylogeny and transmission of Echinococcus. Exp Parasitol 119:439–446
Thompson RCA, McManus D (2002) Towards a taxonomic revision of the genus Echinococcus. Trends Parasitol 18:452–457
Thompson RC, Lymbery AJ, Constantine CC (1995) Variation in Echinococcus: towards a taxonomic revision of the genus. Adv Parasitol 35:145–176
Zhang L, Eslami A, Hosseini SH, McManus DP (1998) Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mitochondrial DNA markers. Am J Trop Med Hyg 59:171–174
Acknowledgements
The authors would like to appreciate all the people who helped to perform this study including Mrs. N. Jalalizand for her kind contribution in molecular lab and the surgeons in Al-Zahra Hospital who had kind cooperation for collecting hydatid cysts samples. This study was financially supported by the National Institute of Health Research, Academic Pivot for Education and Research, Tehran University of Medical Sciences: Project No: 240/5142.
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Kia, E.B., Rahimi, H., Sharbatkhori, M. et al. Genotype identification of human cystic echinococcosis in Isfahan, central Iran. Parasitol Res 107, 757–760 (2010). https://doi.org/10.1007/s00436-010-1947-2
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DOI: https://doi.org/10.1007/s00436-010-1947-2