Abstract
Neurocysticercosis (NC) is the most important neurological disease of parasitic origin in humans. IgA and IgG detection in serum from neurocysticercosis patients was tested using some antigenic preparations of total saline extract from Taenia saginata: detergent (D) and aqueous (A) phases extracted with Triton X-114 and the jacalin bound (JBF) and unbound fractions (JUF) obtained by affinity chromatography using jacalin column. Samples were obtained from 45 patients with definitive NC, who were subdivided into active-NC group and inactive-NC group; 35 patients with other parasitoses; and 30 apparently healthy individuals. Sensitivity and specificity were calculated. Specificity to detect IgA and IgG for D phase, respectively, were 89.8% and 86.9% and for IgG detection 91.3% and 76.8% when using D phase and JUF, respectively. D phase and JBF proved to be specific and efficient and could be efficiently utilized as an alternative antigen for IgA detection in NC, with comparable results with IgG.
References
Baermann S (1917) Eine Einfache Methode zur Auffindung Von Ankylostomum (Nematoden)—Larven in Erdproden. Mededeel mit. h. Geneesk. Lab Weltevredem Feestbundel, Batavia
Bueno EC, Vaz AJ, Machado LD, Livramento JA (2000) Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens. Arq Neuro-psiquiatria 58:18–24
Costa JM, Ferreira AW, Makino MM, Camargo ME (1982) Spinal fluid immunoenzymatic assay (ELISA) for neurocysticercosis. Rev Inst Med Trop São Paulo 24:337–341
Dabes-Guimaraes TM, Toledo VD, Costa CA, Costa RT, Genaro O, Williams P, Mayrink W (1996) Assessment of immunity induced in mice by glycoproteins derived from different strains and species of Leishmania. Mem Inst Oswaldo Cruz 91:63–70
Del Brutto OH, Wadia NH, Dumas M, Cruz M, Tsang VCW, Schantz PM (1996) Proposal of diagnostic criteria for human cysticercosis and Neurocysticercosis. J Neurol Sci 142:1–6
Dua T, Aneja S (2006) Neurocysticercosis: management issues. Indian Pediatr 43:227–235
Enseñat J, Martínez-Mañas R, Horcajada JP, De Juan C, Ferrer E (2007) Diagnostic and therapeutic difficulties in neurocysticercosis: presentation of 6 cases and review of the literature. Neurocirurgia (Astur) 18:101–110
Espinoza B, Ruiz-Palacios G, Tovar A, Sandoval MA, Plancarte A, Flisser A (1986) Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis. J Clin Microbiol 24:536–541
Garcia HH, González AE, Evans CAV, Gilman RH (2003) Taenia solium cysticercosis. Lancet 361:547–556
Gomes-Silva A, Souza MA, Afonso-Cardoso SR, Andrade LR, Dietze R, Lemos E (2008) Serological reactivity of different antigenic preparations of Leishmania (Leishmania) amazonensis and the Leishmania braziliensis complex. Rev Soc Bras Med Trop 41(2):135–141
Ishida MMI, Peralta RHS, Livramento JA, Hoshino-Shimizu S, Peralta JM, Vaz AJ (2006) Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay. Rev Inst Med Trop São Paulo 48:343–346
Letonja T, Hammerberg C, Schurig G (1987) Evaluation of spleen lymphocyte responsiveness to a T-cell mitogen during early infection with larval Taenia taeniaeformis. Parasitol Res 73:265–270
Lowry VH, Rosebrouch NJ, Farr AL, Randal RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Lutz AV (1919) Schistosoma mansoni e a schistosomose, segundo observações feitas no Brasil. Mem Inst Oswaldo Cruz 11:121–125
Machado GA, Santiago FM, Mineo JR, Costa-Cruz JM (2007) Assessment of antigenic fractions of varying hydrophobicity from Taenia solium metacestodes for the diagnosis of human Neurocysticercosis. Trop Med Int Health 12:1–8
Mandal J, Singhi PD, Khandelwal N, Malla N (2008) Evaluation of lower molecular mass (20-24 kDa) Taenia solium cysticercosis antigen fraction by ELISA and dot blot for the serodiagnosis of neurocysticercosis in children. Parasitol Res 102:1097–1101
Mineo JR, Silva DAO, Sopelete MC, Leal GS, Vidigal LHG, Tápia LER, Bacchin MI (2005) Pesquisa na área biomédica: do planejamento à publicação. EDUFU: Editora da Universidade Federal de Uberlândia, Uberlândia, p 273
Odashima NS, Takayanagui OM, Figueiredo JF (2002) Enzyme linked immunosorbent assay (ELISA) for the detection of IgG, IgM, IgE and IgA against Cysticercus cellulosae in cerebrospinal fluid of patients with neurocysticercosis. Arq Neuro-Psiquiatria 60:400–405
Oliveira HB, Machado GA, Cabral DD, Costa-Cruz JM (2007) Application of Taenia saginata metacestodes as an alternative antigen for the serological diagnosis of human neurocysticercosis. Parasitol Res 101:107–113
Sahu PS, Parija SC, Sahu PK (2008) Tear IgA-ELISA novel and sensitive method for diagnosis of ophthalmic cysticercosis. Acta Trop 106:168–174
Sako Y, Nakao M, Nakaya K, Yamasaki H, Ito A (2006) Recombinant antigens for serodiagnosis of cysticercosis and echinococcosis. Parasitol Int 55:69–73
Shiguekawa KYM, Mineo JR, Moura LP, Costa-Cruz JM (2000) ELISA and western blotting tests in the detection of IgG antibodies to Taenia solium metacestodes in serum samples in human neurocysticercosis. Trop Med Int Health 5:443–449
Sotelo J, Guerrero V, Rubio F (1985) Neurocysticercosis: a new classification based on active and inactive forms. A study of 753 cases. Arch Intern Med 145:442–445
van Egmond M, Damen CA, van Spriel AB, Vidarsson G, van Garderen E, van de Winkel JG (2001) IgA and the IgA Fc receptor. Trends Immunol 22:205–211
Yoo EM, Morrison SL (2005) IgA: an immune glycoprotein. Clin Immunol 116:3–10
Acknowledgments
Financial support. This study was supported by the Brazilian Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG) and the Federal University of Uberlândia (UFU), Brazil.
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da S. Ribeiro, V., Manhani, M.N. & Costa-Cruz, J.M. IgA detection in human neurocysticercosis using different preparations of heterologous antigen. Parasitol Res 107, 221–225 (2010). https://doi.org/10.1007/s00436-010-1859-1
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DOI: https://doi.org/10.1007/s00436-010-1859-1