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Expression and functional characterization of a Rho-family small GTPase CDC42 from Trichinella spiralis

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Abstract

A full-length cDNA encoding a Rho-family small GTPase gene cdc42 of Trichinella spiralis was expressed in E. coli. The recombinant protein of TsCDC42 was purified and used to raise the polyclonal antibodies. The expression of TsCDC42 in different stages of parasite was investigated. The western blot showed that TsCDC42 was expressed in all stages of T. spiralis, including newborn larvae, muscle larvae and adult worms. The immuno-localization revealed that TsCDC42 was ubiquitously distributed in the newborn larvae, muscle larvae and adult worm. Cross-species RNAi was done by knockdown Tscdc42 RNAi in C. elegans. The results revealed that endogenous expression level of CDC42 was decreased by knockdown Tscdc42 RNAi in C. elegans, and this knockdown reduced the progeny of C. elegans. It suggested that Tscdc42 might play the same roles in the early development of T. spiralis.

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Acknowledgements

This work was supported by Xiamen Science Technology grant (3502Z20071077), China National Nature Science Foundation (No. 30972181) and New Century Talents Support Program from Xiamen University to YRY. We thank Dr. Xin-zhuan Su for manuscript revision and comments. Ceanorhabditis elegans wild-type animals (N2) and E. coli (HT115) were gifts from Ceanorhabditis Genetics Center. L4440 vector was generously provided by Andy Fire Lab through Addgene Co.

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  1. Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Science, Xiamen University, Xiamen, Fujian, 361005, People’s Republic of China

    Yurong Yang, Weiwen Qin, Guimei Tian & Wei Jian

  2. Department of Biology, School of Life Science, Xiamen University, Xiamen, Fujian, 361005, People’s Republic of China

    Yurong Yang

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  1. Yurong Yang
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  2. Weiwen Qin
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Correspondence to Yurong Yang.

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Yang, Y., Qin, W., Tian, G. et al. Expression and functional characterization of a Rho-family small GTPase CDC42 from Trichinella spiralis . Parasitol Res 107, 153–162 (2010). https://doi.org/10.1007/s00436-010-1851-9

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  • DOI: https://doi.org/10.1007/s00436-010-1851-9

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