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Efficacy of a combination product containing pyrantel, febantel and praziquantel (Drontal® Plus Flavour, Bayer Animal Health) against experimental infection with the hookworm Ancylostoma ceylanicum in dogs

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Abstract

Ancylostoma ceylanicum is a common hookworm of dogs, cats and humans in Asia. More recently, this hookworm was found to infect dogs in Australia. The objective of this study was to determine the efficacy of a combination product containing pyrantel, febantel and praziquantel (Drontal® Plus Flavour, Bayer) against A. ceylanicum in experimentally infected dogs. Twelve dogs were each subcutaneously injected with 300 infective third-stage larvae of A. ceylanicum. Pups were stratified by egg count and randomly allocated equally into control and treatment groups. The pups in the treatment group were treated orally at 20 days post-infection with a tablet containing pyrantel, febantel and praziquantel (Drontal® Plus Flavour, Bayer) with the recommended dose of one tablet per 10 kg bodyweight. The dogs in the control group were not treated. Egg counts were performed daily until the end of the study period and compared for the treated and control groups. No eggs were detected in the treated group of pups within 3 days of treatment, and faecal samples from this group remained negative throughout the rest of the study resulting in a treatment efficacy (egg reduction) of 100% (p = 0.0011). The egg counts for the untreated group remained high for the rest of the study period. This trial demonstrated that a combination tablet containing pyrantel, febantel and praziquantel (Drontal® Plus Flavour, Bayer) given at the manufacturer’s recommended dose is effective against infection with A. ceylanicum in dogs.

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References

  • Anten JF, Zuidema PJ (1964) Hookworm infection in Dutch servicemen returning from West New Guinea. Trop Geogr Med 64:216–224

    CAS  PubMed  Google Scholar 

  • Areekul S, Radomyos P, Viravan C (1970a) Experimental infection of Ancylostoma ceylanicum in man. J Med Assoc Thai 53:190–194

    CAS  PubMed  Google Scholar 

  • Areekul S, Radomyos P, Viravan C (1970b) Preliminary report of Ancylostoma ceylanicum infection in Thai people. J Med Assoc Thai 53:315–321

    CAS  PubMed  Google Scholar 

  • Areekul S, Saenghirun C, Ukoskit K (1975) Studies on the pathogenicity of Ancylostoma ceylanicum. I. Blood loss in experimental dogs. Southeast Asian J Trop Med Public Health 6:235–240

    CAS  PubMed  Google Scholar 

  • Carroll SM, Grove DI (1984) Parasitological, hematologic, and immunologic responses in acute and chronic infections of dogs with Ancylostoma ceylanicum: a model of human hookworm infection. J Infect Dis 150:284–294

    CAS  PubMed  Google Scholar 

  • Carroll SM, Grove DI (1986) Experimental infection of humans with Ancylostoma ceylanicum: clinical, parasitological, haematological and immunological findings. Trop Geogr Med 38:38–45

    CAS  PubMed  Google Scholar 

  • Choo J, Pang E, Prociv P (2000) Hookworms in dogs of Kuching, Sarawak (north Borneo). Trans R Soc Trop Med Hyg 94:21–22

    Article  CAS  PubMed  Google Scholar 

  • Chowdhury AB, Schad GA (1972) Ancylostoma ceylanicum: a parasite of man in Calcutta and environs. Am J Trop Med Hyg 21:300–301

    CAS  PubMed  Google Scholar 

  • Hopkins TJ, Gyr P (1991) Synergism of a combination of febantel and pyrantel embonate against Ancylostoma caninum on dogs. Vet Med Rev 61:3–9

    Google Scholar 

  • Kopp SR, Kotze AC, McCarthy JS, Coleman GT (2007) High-level pyrantel resistance in the hookworm Ancylostoma caninum. Vet Parasitol 143:299–304

    Article  CAS  PubMed  Google Scholar 

  • Kopp SR, Kotze AC, McCarthy JS, Traub RJ, Coleman GT (2008) Pyrantel in small animal medicine: 30 years on. Vet J 178:177–184

    Article  CAS  PubMed  Google Scholar 

  • Lachin JM (1992) Some large-sample distribution-free estimators and tests for multivariate partially incomplete data from two populations. Stat Med 11:1151–1170

    Article  CAS  PubMed  Google Scholar 

  • Margono SS, Koesharjono C, Kosin E (1979) Hookworm in dogs and cats in the area of Jakarta. Trop Geogr Med 31:257–261

    CAS  PubMed  Google Scholar 

  • Miller TA (1966) Blood loss during hookworm infection, determined by erythrocyte labeling with radioactive 51-chromium. II. Pathogenesis of Ancylostoma braziliense infection in dogs and cats. J Parasitol 52:856–865

    Article  CAS  PubMed  Google Scholar 

  • Miller TA (1968) Pathogenesis and immunity in hookworm infection. Trans R Soc Trop Med Hyg 62:473–489

    Article  CAS  PubMed  Google Scholar 

  • Palmer CS, Traub RJ, Robertson ID, Hobbs RP, Elliot A, While L, Rees R, Thompson RC (2007) The veterinary and public health significance of hookworm in dogs and cats in Australia and the status of A. ceylanicum. Vet Parasitol 145:304–313

    Article  PubMed  Google Scholar 

  • Pratt PW (1997) Laboratory procedure for veterinary technicians, 3r edn. Mosby, St. Louis

  • Ray DK, Bhopale KK, Shrivastava VB (1972) Migration and growth of Ancylostoma ceylanicum in golden hamsters Mesocricetus auratus. J Helminthol 46:357–362

    Article  CAS  PubMed  Google Scholar 

  • Rep BH, van Joost KS, Vetter JC (1971) Pathogenicity of Ancylostoma ceylanicum. VI. Lethal blood loss in hookworm infection. Trop Geogr Med 23:184–193

    CAS  PubMed  Google Scholar 

  • Rodkhum C, Runglertkriangkrai N, Thaweethavonsawat P, Nithiuthai S, Preechatangkit B, Kositamongkol T, Chungpivat S (1997) Comparative studies on the anthelmintic efficacy of nitroscanate mebendazone and a combination of pyrantel, febantel and praziquantel against hookworms and ascarid in puppies. Thai J Vet Med 27:121–137

    Google Scholar 

  • Setasuban P, Vajrasthira S, Muennoo C (1976) Prevalence and zoonotic potential of Ancylostoma ceylanicum in cats in Thailand. Southeast Asian J Trop Med Public Health 7:534–539

    CAS  PubMed  Google Scholar 

  • Sheikh O, Lee C, Whitten L (1985) A survey of helminths and arthropods in stray dogs in Kuala Lumpur. Kajian Vet J 17:19–23

    Google Scholar 

  • Taweethavonsawat P, Chungpivat S, Viseshakul N (2007) Update: status of intestinal parasites in stray dogs in Bangkok. Joint International Tropical and Medicine Meeting, "Health Security in the Tropics".

  • Thall PF, Lachin JM (1988) Analysis of recurrent events: nonparametric methods for random interval count data. J Am Stat Assoc 83:339–347

    Article  Google Scholar 

  • Traub RJ, Hobbs RP, Adams PJ, Behnke JM, Harris PD, Thompson RC (2007) A case of mistaken identity—reappraisal of the species of canid and felid hookworms (Ancylostoma) present in Australia and India. Parasitology 134:113–119

    Article  CAS  PubMed  Google Scholar 

  • Traub RJ, Inpankaew T, Sutthikornchai C, Sukthana Y, Thompson RC (2008) PCR-based coprodiagnostic tools reveal dogs as reservoirs of zoonotic ancylostomiasis caused by Ancylostoma ceylanicum in temple communities in Bangkok. Vet Parasitol 155:67–73

    Article  CAS  PubMed  Google Scholar 

  • Velasquez CC, Cabrera BC (1968) Ancylostoma ceylanicum (Looss, 1911) in a Filipino woman. J Parasitol 54:430–431

    Article  CAS  PubMed  Google Scholar 

  • VICH guideline: 19 (2001) Efficacy for anthelmintics: specific requirements for canines. Veterinary International Cooperation on Harmonization of Technical Requirements for Registration of Veterinary Medicinal Products.

  • Wei LJ, Lachin JM (1984) Two-sample asymptotically distribution-free tests for incomplete multivariate observations. J Am Stat Assoc 79:653–661

    Article  Google Scholar 

  • Wijers DJ, Smit AM (1966) Early symptoms after experimental infection of man with Ancylostoma braziliense var. ceylanicum. Trop Geogr Med 18:48–52

    CAS  PubMed  Google Scholar 

  • Yoshida Y (1971) Comparative studies on Ancylostoma braziliense and Ancylostoma ceylanicum. I. The adult stage. J Parasitol 57:983–989

    Article  CAS  PubMed  Google Scholar 

  • Yoshida Y, Okamoto K, Chiu JK (1968) Ancylostoma ceylanicum infection in dogs, cats, and man in Taiwan. Am J Trop Med Hyg 17:378–381

    CAS  PubMed  Google Scholar 

  • Yoshida Y, Okamoto K, Matsuo K, Kwo EH, Retnasabapathy A (1973) The occurrence of Ancylostoma braziliense (de Faria, 1910) and Ancylostoma ceylanicum (Looss, 1911) in Malaysia. Southeast Asian J Trop Med Public Health 4:498–503

    CAS  PubMed  Google Scholar 

  • Yoshida Y, Kondo K, Kurimoto H, Fukutome S, Shirasaka S (1974) Comparative studies on Ancylostoma braziliense and Ancylostoma ceylanicum. 3. Life history in the definitive host. J Parasitol 60:636–641

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This study was supported by Bayer Healthcare Animal Health Thailand and Australia.

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Authors and Affiliations

  1. Parasitology Unit, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Piyanan Taweethavonsawat & Sudchit Chungpivat

  2. Bayer Healthcare Animal Health, Bayer Thailand, Bangkok, Thailand

    Pakkawan Satranarakun

  3. School of Veterinary Science, The University of Queensland, St Lucia, Queensland, 4072, Australia

    Rebecca J. Traub

  4. Bayer Animal Health GmbH, Leverkusen, Germany

    Roland Schaper

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  1. Piyanan Taweethavonsawat
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  2. Sudchit Chungpivat
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  3. Pakkawan Satranarakun
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  4. Rebecca J. Traub
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  5. Roland Schaper
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Correspondence to Piyanan Taweethavonsawat.

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Taweethavonsawat, P., Chungpivat, S., Satranarakun, P. et al. Efficacy of a combination product containing pyrantel, febantel and praziquantel (Drontal® Plus Flavour, Bayer Animal Health) against experimental infection with the hookworm Ancylostoma ceylanicum in dogs. Parasitol Res 106, 533–537 (2010). https://doi.org/10.1007/s00436-009-1665-9

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  • DOI: https://doi.org/10.1007/s00436-009-1665-9

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