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Mosquito blood-meal analysis for avian malaria study in wild bird communities: laboratory verification and application to Culex sasai (Diptera: Culicidae) collected in Tokyo, Japan

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Abstract

We conducted laboratory experiments to verify molecular techniques of avian malaria parasite detection distinguishing between an infected mosquito (oocysts on midgut wall) and infective mosquito (sporozoites in salivary glands) in parallel with blood-meal identification from individual blood-fed mosquitoes prior to application to field survey for avian malaria. Domestic fowl infected with Plasmodium gallinaceum was exposed to a vector and non-vector mosquito species, Aedes aegypti and Culex pipiens pallens, respectively, to compare the time course of polymerase chain reaction (PCR) detection for parasite between competent and refractory mosquitoes. DNA of the domestic fowl was detectable for at least 3 days after blood feeding. The PCR-based detection of P. gallinaceum from the abdomen and thorax of A. aegypti corresponded to the microscopic observation of oocysts and sporozoites. Therefore, this PCR-based method was considered useful as one of the criteria to assess developmental stages of Plasmodium spp. in mosquito species collected in the field. We applied the same PCR-based method to 21 blood-fed C. sasai mosquitoes collected in Rinshi-no-mori Park in urban Tokyo, Japan. Of 15 blood meals of C. sasai successfully identified, 86.7% were avian-derived, 13.3% were bovine-derived. Plasmodium DNA was amplified from the abdomen of three C. sasai specimens having an avian blood meal from the Great Tit (Parus major), Pale Thrush (Turdus pallidus), and Jungle Crow (Corvus macrorhynchos). This is the first field study on host-feeding habits of C. sasai in relation to the potential role as a vector for avian malaria parasites transmitted in the Japanese wild bird community.

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Acknowledgments

We thank Kyoko Sawabe, Haruhiko Isawa, Shinji Kasai, Keita Hoshino, and Osamu Komagata of the Department of Medical Entomology, National Institute of Infectious Diseases for their help with molecular laboratory work. Thanks are also due to Koichi Murata and Yukita Sato of Nihon University for their encouragement during the study. This work was partly supported by a Grant-in-Aid for Scientific Research of Emerging and Reemerging Infectious Diseases from the Ministry of Health, Labor, and Welfare of the Japanese Government (H18-Shinko-009).

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Correspondence to Kyeong Soon Kim.

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Kim, K.S., Tsuda, Y., Sasaki, T. et al. Mosquito blood-meal analysis for avian malaria study in wild bird communities: laboratory verification and application to Culex sasai (Diptera: Culicidae) collected in Tokyo, Japan. Parasitol Res 105, 1351–1357 (2009). https://doi.org/10.1007/s00436-009-1568-9

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  • DOI: https://doi.org/10.1007/s00436-009-1568-9

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