Abstract
The detection of two human-pathogenic Giardia duodenalis assemblages A and B in faecal and water samples by loop-mediated isothermal amplification (LAMP) has been evaluated. The LAMP reaction is reproducible, rapid and specific for the detection G. duodenalis and has lower costs compared to the other molecular assays. This is the first application of LAMP for Giardia detection.
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Acknowledgements
This study was supported by Grant-in-Aid for Young Scientists, Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science and from the twenty-first Century COE Programme (A-1), Ministry of Education, Sports, Science, and Technology of Japan. We would like to thank Dr Jerry Ongerth for his editorial review and suggestions for the manuscript.
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Plutzer, J., Karanis, P. Rapid identification of Giardia duodenalis by loop-mediated isothermal amplification (LAMP) from faecal and environmental samples and comparative findings by PCR and real-time PCR methods. Parasitol Res 104, 1527–1533 (2009). https://doi.org/10.1007/s00436-009-1391-3
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DOI: https://doi.org/10.1007/s00436-009-1391-3