Abstract
Gastrodiscoides hominis (Digenea: Paramphistomata: Gastrodiscidae) is an amphistomid intestinal fluke of pigs causing gastrodiscoidiosis. With the use of molecular tools assisting the conventional diagnostic procedures, we aimed at finding out molecular characterization of G. hominis using PCR amplifications of rDNA ITS (1, 2) sequences. The sequences obtained (GenBank accession numbers EF027096, EF027097, EF027098, EU887294, and EU887295) were compared with available sequences of other digenean parasites, particularly those having a zoonotic potential in the northeastern region of India. The BLAST search revealed a close similarity with members of the family Paramphistomidae, showing maximum similarity with the amphistome, Homalogaster paloniae (subfamily Paramphistominae). Based on various tree construction methods, phylogeny of G. hominis is discussed.
Similar content being viewed by others
References
Ahluwalia SS (1960) Gastrodiscoides hominis (Lewis and McConnell, 1876) Leiper, 1913—the amphistome parasite of man and pig. Indian J Med Res 48:315–325
Blair D, Campos A, Cummings MP, Laclette JP (1996) Evolutionary biology of parasitic platyhelminths: the role of molecular phylogenetics. Parasitol Today 12:66–71
Blair D, Wu B, Chang ZS, Gong X, Agatsuma T, Zhang YN, Chen SH, Lin JX, Chen MG, Waikagui J, Guevara AG, Feng Z, David GM (1999) A molecular perspective on the genera Paragonimus Braun, Euparagonimus Chen and Pagumogonimus Chen. J Helminthol 74:295–299
Bowles J, Blair D, McManus DP (1995) A molecular phylogeny of the human schistosomes. Mol Phylogenet Evol 4:103–109
Buckley JJC (1939) Observations on Gastrodiscoides hominis and Fasciolopsis buski in Assam. J Helminthol 17:1–12
Buckley JJC (1964) The problem of Gastrodiscoides hominis (Lewis and McConell, 1876) Leiper, 1913. J Helminthol 38(1/2):1–6
Dada-Adegbola HO, Falade CO, Oliwatoba OA, Abiodun OO (2004) Gastrodiscoides hominis infection in a Nigerian—case report. West African J Med 23:185–186
Dutt SC, Srivastava HD (1972) The life history of Gastrodiscoides hominis (Lewis and McConnel, 1876) Leiper, 1913, the amphistome parasite of man and pig. J Helminthol 46:35–46
Easwaran KR, Reghu R, Pillai KM (2003) Parasitic infection of some wild animals at Thekkady in Kerala. Zoos’ Print J 18:1030
Fox JG, Hall WC (1970) Fluke (Gastrodiscoides hominis) infection in a rhesus monkey with related intussusception of the colon. J Amer Vet Med Assoc 157:714–716
Harinasuta T, Bunnag D, Radomyos P (1987) Intestinal fluke infections. Bailliere Clin Trop Med Commun Dis 2(3):695–721
Herman LH (1967) Gastrodiscoides hominis infestation in two monkeys. Vet Med Small Anim Clin 62:355–356
Hillis DM, Dixon MT (1991) Ribosomal DNA: molecular evolution and phylogenetic inference. Q Rev Biol 66:411–453
Hust J, Frydenberg J, Sauriau PG, Gall PL, Mouritsen KN, Jensen KT (2004) Use of ITS rDNA for discriminating of larval stages of two microphallid (Digenea) species using Hydrobia ulvae (Pennant, 1777) and Corophium volutator (Pallas, 1766) as intermediate hosts. Parasitol Res 93:304–310
Itagaki A, Tsumagari N, Tsutsumi K, Chinone S (2003) Discrimination of three amphistome species by PCR-RFLP based on rDNA ITS2 markers. J Vet Med Sci 65:931–933
Ivanov VM, Semenova NN (2000) Parasitological consequences of animal introduction. Russian J Ecol 31:281–283
Iwagami M, Lo LY, Su K, Lai PF, Fukushima M, Nakano M, Blair D, Kawashima K, Agatsuma T (2000) Molecular phylogeographic studies on Paragonimus westermani in Asia. J Helminthol 74:315–322
Kumar V (1980) The digenetic trematodes, Fasciolopsis buski, Gastrodiscoides hominis Artyfechinostomum malayanum, as zoonotic infections in South Asian countries. Ann Soc Belge Med Trop 60:331–339
Kumar V (1999) Trematode infections and diseases of man and animals. Kluwer, Dordrecht
Luton K, Walker D, Blair D (1992) Comparisons of ribosomal internal transcribed spacers from two congeneric species of flukes (Platyhelminths: Trematoda: Digenea). Mol Biochem Parasitol 56:323–328
Mas-Coma S, Bargues MD, Valero MA (2005) Fascioliasis and other plant-borne trematode zoonoses. Int J Parasitol 35:1255–1278
Mas-Coma S, Bargues MD, Valero AM (2007) Plant-borne trematode zoonoses: fascioliasis and fasciolopsiasis. In: Murrel KD, Fried B (eds) Food borne parasitic zoonoses, vol. 11, part II. Springer, New York, pp 293–334
Miyazaki I (1974) Lung flukes in the world. Morphology and life history. In: Sasa M (ed) A symposium on epidemiology of parasitic diseases. International Medicine Foundation of Japan, Tokyo, pp 101–135
Murty CV, Reddy CR (1980) A case report of Gastrodiscoides hominis infection. Indian J Pathol Microbiol 23:303–304
Prasad PK, Tandon V, Chatterjee A, Bandyopadhyay S (2007) PCR-based determination of internal transcribed spacer (ITS) regions of ribosomal DNA of giant intestinal fluke, Fasciolopsis buski (Lankester, 1857) Looss, 1899. Parasitol Res 101:1581–1587
Roy B, Tandon V (1992) Seasonal prevalence of some zoonotic digenean infections in cattle and pigs in a humid subtropical montane zone in India. Vet Parasitol 41:69–76
Sambrooke J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Shrivastav HO, Shah HL (1970) On Gastrodiscoides hominis (Lewis and McConnell, 1876) Leiper, 1913 from pigs (Sus scrofa domestica) in Madhya Pradesh, its pathology and public health importance. Indian J Pathol Bacteriol 13:68–72
Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
Tandon V, Prasad PK, Chatterjee A, Bhutia PT (2007) Surface fine topography and PCR-based determination of metacercaria of Paragonimus sp. from edible crabs in Arunachal Pradesh, Northeast India. Parasitol Res 102:21–28
Thompson RCA, Zarlenga DS, La Rosa G, Pozio E, Rosenthal B, Bandi C, Mortarino M, Casiraghi M, Genchi C, Gasser RB, Hu M, Chilton NB, Matthews JB, Hodgkinson JE (2004) Advances in the diagnosis and systematics of parasites of veterinary importance: new and exciting prospects. In: Gasser RB, Zarlenga DS (eds). Molecular systematics and diagnosis. Vet Parasitol 125:69–92
White BA (1993) PCR Protocols, current methods and applications. Volume 15. Humana, Totowa
Yu SH, Mott KE (1994) Epidemiology and morbidity of food-borne intestinal trematode infections. Trop Dis Bull 91(7):R125–R152
Zablotski VI (1964) Early development stages of Gastrodiscoides hominis Lewis et McConnell, 1876. Sb Parazit Rab Trudy Astrskh Zapovedn 9:119–126
Acknowledgements
This study was carried out under the Department of Biotechnology-sanctioned project to VT & AC and partly supported by DSA (UGC–SAP) program in the Department of Zoology and UPE-Biosciences program in the School of Life Sciences at NEHU, Shillong. We are also thankful to the Coordinator, Bioinformatics Centre NEHU, for allowing access to its facility.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Goswami, L.M., Prasad, P.K., Tandon, V. et al. Molecular characterization of Gastrodiscoides hominis (Platyhelminthes: Trematoda: Digenea) inferred from ITS rDNA sequence analysis. Parasitol Res 104, 1485–1490 (2009). https://doi.org/10.1007/s00436-009-1354-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-009-1354-8