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Biochemical characterization of deltamethrin resistance in a laboratory-selected strain of Aedes aegypti

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Abstract

A resistant strain (DR) of Aedes aegypti, generated by deltamethrin selection for 20 consecutive generations from a laboratory susceptible strain (DS) was studied for the possible resistant mechanisms. The pyrethroid resistance developed was characterized by biochemical assays and native polyacrylamide gel electrophoresis. Significant elevation in the activity of α- and β-esterases, glucose-6-phosphate dehydrogenase (G6PD), CYTP450 (CYTP450), and glutathione-s-transferase (GST) were noticed in DR. The gel profiles for esterases, G6PD, and CYTP450 were different in DR as compared to DS strain. The difference was either in the form of additional bands or increased intensity of the bands or both. Gel profile variations were also evident from densitometry. Our study suggests that these enzymes play an important role in deltamethrin resistance in the DR strain.

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Acknowledgement

The authors are thankful to the Department of studies in Zoology, University of Mysore, Mysore for providing all the facilities to conduct this research work. One of the authors is thankful to the Council of Scientific and Industrial Research (CSIR), India for the financial assistance.

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Authors and Affiliations

  1. School of Biological Sciences, Washington State University, Pullman, WA, USA

    Urmila Jagadeshwaran

  2. Department of Zoology, University of Mysore, Mysore, India

    V. A. Vijayan

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  1. Urmila Jagadeshwaran
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  2. V. A. Vijayan
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Correspondence to Urmila Jagadeshwaran.

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Jagadeshwaran, U., Vijayan, V.A. Biochemical characterization of deltamethrin resistance in a laboratory-selected strain of Aedes aegypti . Parasitol Res 104, 1431–1438 (2009). https://doi.org/10.1007/s00436-009-1342-z

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  • DOI: https://doi.org/10.1007/s00436-009-1342-z

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