Abstract
Strains of Acanthamoeba sp. constitute a factor contributing to the occurrence of chronic granulomatous amoebic encephalitis, keratitis, pneumonia, as well as inflammations of other organs. Treatment of these diseases is very difficult and not always effective. A majority of these infections have been fatal. The aim of our study was to examine the amoebicidal or amoebistatic activity of plant extracts from Rubus chamaemorus, Pueraria lobata, Solidago virgaurea and Solidago graminifolia. For the purpose of isolation of pharmacologically active substances, we used the aboveground parts of plants, together with flowers, roots and leaves. It was established that extracts from S. virgauera, P. lobata and R. chamaemorus displayed chemotherapeutic properties in vitro in concentrations of approximately 0.01–0.05 mg extract/mL, i.e. in concentrations of 0.350 μg/mL expressed in ellagic acid for R. chamaemorus and 0.053 μg/mL expressed in puerarin for P. lobata. Therapeutic index values is 3.5–20. As a result of in vivo experiments, it was found out that, following therapy using the extracts, animals infected with Acanthamoeba sp. survived for an extended period (2.5–3 times longer). It was determined that plant extracts may be used both externally and internally in the case of a combined therapy for acanthamoebiasis. The tested extracts are not toxic for animals.
References
Arrieta J, Reyes B, Calzada F, Cedillo-Rivera R (2001) Amoebicidal and giardicidal compounds from the leaves of Zanthoxylum liebmannianun. Fitoterapia 72:295–297
Callicott JG (1968) Amebic meningoencephalitis due to free-living amebas of the Hartmannella (Acanthamoeba)–Naegleria group. Am J Clin Pathol 49:84–91
Červa L (1966) Use of fluorescent antibody technique to identify pathogenic Hartmannellae in tissue of experimental animals. Folia Parasitol (Praha) 13:328–331
ÄŚerva L (1969) Amoebic meningoencephalitis: axenic culture of Naegleria. Science 163:576
De Jonckheere JF (1991) Ecology of Acanthamoeba. Rev Infect Dis 13:S385–S387
Derda M, Hadaś E, Thiem B, Sułek A (2004a) Amebicidal plant extracts. Wiad Parazytol 50:715–721
Derda M, Hadaś E, Thiem B, Sułek A (2004b) Natural products as amebicidal drugs in acanthamoebosis. Acta Pol Pharm 61:24–26
Dougherty PJ, Binder PS, Mondino BJ, Glasgow BJ (1994) Acanthamoeba sclerokeratitis. Am J Ophthalmol 117:475–479
Ficker L, Seal D, Warhurst D, Wright P (1990) Acanthamoeba keratitis—resistance to medical therapy. Eye 4:835–838
Fowler M, Carter RF (1965) Acute pyogenic meningitis probably due to Acanthamoeba sp.: a preliminary report. Br Med J 2:740–742
Horne DD, Frizell ME, Ingam L, Janas RG, Gubash SM, Anand CM, Athar MA (1994) Acanthamoeba keratitis an emerging clinical problem. Can Med Assoc J 150:923–925
Jager BV, Stamm WP (1972) Brain abscesses caused by free-living amoeba probably of the genus Hartmannella in a patient with Hodgkins disease. Lancet 23:1343–1345
Kasprzak W, Mazur T (1972) Free living amoebae isolated from waters frequented by people in the vicinity of Poznań, Poland. Experimental studies in mice on the pathogenicity of the isolates. Z Tropenmed Parasitol 23:391–398
Kayser O, Kiderlen AF, Croft SL (2003) Natural products as antiparasitic drugs. Parasitol Res 90:55–62
Kitagawa K, Nakamura T, Takahashi N, Oikawa Y, Ikeda T (2003) A novel combination treatment of Chlorohexidine gluconate, natamycin (pimaricin) and debridement for a Acanthamoeba keratitis. Jpn J Ophthalmol 47:616–617
Krawczyk A, Thiem B, Szkudlarek M (2003) High-performance liquid chromatography of ellagic acid in leaves of Rubus chamaemorus L. Chem Anal 48:891–899
Marciano-Cabral F, Cabral G (2003) Acanthamoeba spp. as agents of disease in humans. Clin Microbiol Rev 16:273–307
Marciano-Cabral F, Puffenbarger R, Cabral G (2000) The increasing importance of Acanthamoeba infections. J Eukaryot Microbiol 47:29–36
Martinez AJ, Visvesvara GS (1997) Free-living, amphizoic and opportunistic amebas. Brain Pathol 7:583–598
Martinez AJ, Sotelo-Avila C, Garcia-Tamayo J, Moron JT, Willaert E, Stamm WP (1977) Meningoencephalitis due to Acanthamoeba sp. Pathogenesis and clinico-pathological study. Acta Neuropathol 37:183–191
Mazur T (1984) Występowanie Naegleria fowleri w środowisku wolnym i właściwości biologiczne izolowanych szczepów. Wiad Parazytol 30:3–35
Mergeryan H (1991) The prevalence of Acanthamoeba in the human environment. Rev Infect Dis 13:S390–S391
Murdoch D, Gray TB, Cursons R, Parr D (1998) Acanthamoeba keratitis in New Zealand, including two cases with in vitro resistance to polyhexamethylene biguanide. Aust N Z J Ophthalmol 26:231–236
Said Fernández S, Ramos Guerra MC, Marta Cárdenas BD, Vargas Villarreal J, Villarreal Treviño L (2005) In vitro antiprotozoal activity of the leaves of Artemisia ludoviciana. Fitoterapia 76:466–468
Seal DV (2003) Acanthamoeba keratitis update—incidence, molecular epidemiology and new drugs for treatment. Eye 17:893–905
Szenasi Z, Endo T, Yagita K, Nagy E (1998) Isolation, identification and increasing importance of “free-living” amoebae causing human disease. J Med Microbiol 47:5–16
Visvesvara GS, Stehr-Green J (1990) Epidemiology of free-living ameba infections. J Protozool 37:25S–33S
Willaert E, Stevens AR, Healy GR (1976) Indirect immunofluorescent identification of Acanthamoeba causing meningoencephalitis. Pathol Biol (Paris) 24:545–547
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Derda, M., Hadaś, E. & Thiem, B. Plant extracts as natural amoebicidal agents. Parasitol Res 104, 705–708 (2009). https://doi.org/10.1007/s00436-008-1277-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-008-1277-9