Abstract
A comparative efficacy of four antibiotics, plasmocin (macrolid), Biomyc-1, -2, (tetracycline), and Biomyc-3, and Mycoplasma Removing Agent (quinolone derivatives) was determined for elimination of mycoplasma from Plasmodium falciparum culture. Presence of mycoplasma was detected using enzyme-PCR-based mycoplasma detection kit and survival of malaria parasite was determined in Giemsa’s stained smear made from treated and untreated cultures. It was observed that a combination of Biomyc-1 and -2 killed malaria parasites within 24 h, whereas plasmocin and Biomyc-3 caused slow death of malaria parasite stretched over a period of 6 days. The only compound which did not kill malaria parasite and eradicated mycoplasma from P. falciparum culture was observed to be MRA.
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Acknowledgements
The authors are grateful to the Indian Council of Medical Research, New Delhi for financial support in the form of SRF to Mrs. Shubhra Srivastava. The CDRI communication no. is 7532.
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Singh, S., Puri, S.K. & Srivastava, K. Treatment and control of mycoplasma contamination in Plasmodium falciparum culture. Parasitol Res 104, 181–184 (2008). https://doi.org/10.1007/s00436-008-1181-3
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DOI: https://doi.org/10.1007/s00436-008-1181-3