Skip to main content
Springer Nature Link
Log in

Interaction of BOP1, a protein for ribosome biogenesis, with EB1 in Giardia lamblia

  • Short Communication
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

A search for a protein(s) interacting with the block of proliferation 1 (Bop1) protein of Giardia lamblia led to the isolation of end binding (EB1) protein 1, a protein that is known to function as a microtubule (MT) plus-end-tracking protein in other organisms. Interaction between Bop1 and EB1 of G. lamblia was confirmed by co-immunoprecipitation of in vitro-synthesized Bop1 and EB1 proteins. This result suggests coordination between ribosome biogenesis and cell cycle-related processes such as mitosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  • Berrueta L, Kraeft SK, Tirnauer JS, Schuyler SC, Chen LB, Hill DE, Pellman D, Bierer BE (1998) The adenomatous polyposis coli-binding protein EB1 is associated with the cytoplasmic and spindle microtubules. Proc Natl Acad Sci USA 95:10596–10601

    Article  PubMed  CAS  Google Scholar 

  • Bernander R, Palm JE, Svard SG (2001) Genomic ploidy in different stages of the Giardia lamblia life cycle. Cell Microbiol 3:55–62

    Article  PubMed  CAS  Google Scholar 

  • Dai MS, Lu H (2004) Inhibition of MDM2-mediated p53 ubiquitination and degradation by ribosomal protein L5. J Biol Chem 279:44475–44482

    Article  PubMed  CAS  Google Scholar 

  • Dawson SC, Sagolia MS, Mancuso JJ, Woessner DJ, House SA, Friz-Laylin L, Cande WZ (2007) Kinesin-13 regulates flagellar, interphase, mitotic microtubule dynamics in Giardia intestinalis. Eukaryot Cell 6:2354–2364

    Article  PubMed  CAS  Google Scholar 

  • Elmendorf HG, Dawson SC, McCaffery JM (2003) The cytoskeleton of Giardia lamblia. Int J Parasitol 33:3–28

    Article  PubMed  Google Scholar 

  • Gimeno RE, Espenshade P, Kaiser CA (1996) COPII coat subunit interactions: Sec24p and Sec23p bind to adjacent regions of Sec16p. Mol Biol Cell 7:1815–1823

    PubMed  CAS  Google Scholar 

  • Gyuris J, Golemis E, Chertkov H, Brent R (1993) Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2. Cell 75:791–803

    Article  PubMed  CAS  Google Scholar 

  • Holzel M, Rohrmoser M, Schlee M, Grimm T, Harasim T, Malamoussi A, Gruber-Eber A, Kremmer E, Erik D (2005) Mammalian WDR12 is a novel member of the Pes–Bop1 complex and is required for ribosome biogenesis and cell proliferation. J Cell Biol 170:367–378

    Article  PubMed  Google Scholar 

  • Kane AV, Ward HD, Keusch GT, Pereira MEA (1991) In vitro encystation of Giardia lamblia: large-scale production of in vitro cysts and strain and clone differences in encystation efficiency. J Parasitol 77:974–981

    Article  PubMed  CAS  Google Scholar 

  • Kaplan KB, Burds AA, Swedlow JR, Bekir SS, Sorger PK, Nathke IS (2001) A role of the adenomatous polyposis coli protein in chromosome segregation. Nature Cell Biol 3:429–432

    Article  PubMed  CAS  Google Scholar 

  • Keister DB (1983) Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg 77:487–488

    Article  PubMed  CAS  Google Scholar 

  • Killian A, Meur NL, Sesboue R, Bourguignon J, Bougeard G, Gautherot J, Bastard C, Frebourg T, Flaman JM (2004) Inactivation of the RRB1-Pescadillo pathway involved in ribosome biogenesis induces chromosomal instability. Oncogene 23:8579–8602

    Article  Google Scholar 

  • Kim J, Goo SY, Chung HJ, Yang HW, Yong TS, Lee KH, Park SJ (2006) Interaction of b-giardin with the Bop1 protein in Giardia lamblia. Parasit Res 98:138–144

    Article  CAS  Google Scholar 

  • Kirk-Mason KE, Turner MJ, Chakraborty PR (1988) Cloning and sequence of b-tubulin cDNA from Giardia lamblia. Nucleic Acids Res 16:2733

    Article  PubMed  CAS  Google Scholar 

  • Ma J, Ptashne M (1988) Converting a eukaryotic transcriptional inhibitor into an activator. Cell 55:443–446

    Article  PubMed  CAS  Google Scholar 

  • Mowatt MR, Lujan HD, Cotton DB, Bowers B, Yee J, Nash TE, Stibbs HH (1995) Developmentally regulated expression of a Giardia lamblia cyst wall protein gene. Mol Microbiol 15:955–963

    Article  PubMed  CAS  Google Scholar 

  • Pestov DG, Grzeszkiewicz TM, Lau LF (1998) Isolation of growth suppressors from cDNA expression library. Oncogene 17:3187–3197

    Article  PubMed  CAS  Google Scholar 

  • Rohrmoser M, Holzel M, Grimm T, Malamoussi A, Harasim T, Orban M, Pfisterer I, Gruber-Eber A, Kremmer E, Erik D (2007) Interdependence of Pes1, Bop1, and WDR12 controls nucleolar localization and assembly of the PeBoW complex required for maturation of the 60S ribosomal subunit. Mol Cell Biol 27:3682–3694

    Article  PubMed  CAS  Google Scholar 

  • Sagolia MS, Dawson SC, Mancuso JJ, Cande WZ (2006) Three-dimensional analysis of mitosis and cytokinesis in the binucleate parasite Giardia intestinalis. J Cell Sci 119:4889–4900

    Article  Google Scholar 

  • Strezoska Z, Pestov DG, Lau LF (2000) Bop1 is a mouse WD repeat nucleolar protein involved in 28S and 5.8S rRNA processing and 60S ribosome biogenesis. Mol Cell Biol 20:5516–5528

    Article  PubMed  CAS  Google Scholar 

  • Strezoska Z, Pestov DG, Lau LF (2002) Functional inactivation of the mouse nucleolar protein Bop1 inhibits multiple steps in pre-rRNA processing and blocks cell cycle progression. J Biol Chem 277:29617–29625

    Article  PubMed  CAS  Google Scholar 

  • Yu LZ, Birky CW, Adam RD (2002) The two nuclei of Giardia each have complete copies of the genome and are partitioned equationally at cytokinesis. Eukaryot Cell 1:191–199

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgment

This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (R04-2003-000-10023-0).

Author information

Authors and Affiliations

  1. Department of Environmental Medical Biology and Institute of Tropical Medicine, Post Brain Korea 21 Program, Yonsei University College of Medicine, Seoul, 120-752, South Korea

    Juri Kim, Tai-Soon Yong & Soon-Jung Park

  2. Department of Environmental and Tropical Medicine, Kunkuk University School of Medicine, Seoul, South Korea

    Seobo Sim

Authors
  1. Juri Kim
    View author publications

    Search author on:PubMed Google Scholar

  2. Seobo Sim
    View author publications

    Search author on:PubMed Google Scholar

  3. Tai-Soon Yong
    View author publications

    Search author on:PubMed Google Scholar

  4. Soon-Jung Park
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Soon-Jung Park.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, J., Sim, S., Yong, TS. et al. Interaction of BOP1, a protein for ribosome biogenesis, with EB1 in Giardia lamblia . Parasitol Res 103, 1459–1464 (2008). https://doi.org/10.1007/s00436-008-1131-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-008-1131-0

Keywords

Profiles

  1. Juri Kim View author profile