Abstract
Allergens Ani s 1 and Ani s 4 have demonstrated their utility for the diagnosis of the sensitization to larvae of the genus Anisakis. The aim was to determine the number of patients with compatible clinical history, who did not recognize Ani s 1 and Ani s 4, and characterize the allergens responsible for their sensitization. Eighty-four patients were studied by CAP and immunoglobulin E (IgE) immunoblotting. The 12% of the patients recognized allergens different from Ani s 1 and Ani s 4, being half sensitized to a heat-resistant 15-kDa allergen, which was isolated by ethanol fractionation, followed by a hydroxyapatite chromatography and a reversed-phase high-performance liquid chromatography and identified by its amino terminal sequence as Ani s 5. A total of 41 of the 84 patients studied (49%) showed specific IgE to Ani s 5 that was detected among the excretory–secretory products and immunohistochemically located at the excretory gland, ventriculus, and the luminal surface of the intestinal epithelium of the larvae.
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Acknowledgments
We thank Angel Mendizabal for the Anisakis larvae supply, Dr. Javier Varela for the N-terminal amino acid analysis, and David Peck for his help with English usage.
The experiments comply with the current laws of the country in which they have been performed.
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This work was supported by grants PI031442 from FIS, Ministry of Health and ALG2005-05669-CO2-02 from Ministry of Education.
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Caballero, M.L., Moneo, I., Gómez-Aguado, F. et al. Isolation of Ani s 5, an excretory–secretory and highly heat-resistant allergen useful for the diagnosis of Anisakis larvae sensitization. Parasitol Res 103, 1231–1233 (2008). https://doi.org/10.1007/s00436-008-1105-2
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DOI: https://doi.org/10.1007/s00436-008-1105-2