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Acanthamoeba spp. cysts storage in filter paper

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Abstract

A new method of storage of the free-living amoeba, Acanthamoeba, is described. For this purpose, strips of filter paper were impregnated with cysts of amoebas cultivated axenically and transferred into a cryotube. The strips were dehydrated at 30°C for 24 h and after storage at room temperature. The amoeba viability has been evaluated and the cysts remain viable and pure for at least 1 year. Then, our method has been efficient for the storage of cysts during the evaluated period.

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Acknowledgements

This work has been supported by CAPES and Microbiology Department of Universidade Federal do Rio Grande do Sul.

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Authors and Affiliations

  1. Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil

    Claiton José Pens & Marilise Brittes Rott

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  1. Claiton José Pens
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  2. Marilise Brittes Rott
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Correspondence to Marilise Brittes Rott.

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Pens, C.J., Rott, M.B. Acanthamoeba spp. cysts storage in filter paper. Parasitol Res 103, 1229–1230 (2008). https://doi.org/10.1007/s00436-008-1093-2

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  • DOI: https://doi.org/10.1007/s00436-008-1093-2

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