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Bile components and amino acids affect survival of the newly excysted juvenile Clonorchis sinensis in maintaining media

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Abstract

Clonorchis sinensis thrives on bile juice. The effects of bile and bile acids on newly excysted juvenile C. sinensis (CsNEJ) were studied in terms of survival. Survival of CsNEJs maintained in 1× Locke’s solution, Dulbecco’s modified Eagle’s medium, NCTC 109, Eagle’s, RPMI 1640, and 0.1% glucose was high, but dropped rapidly in 2× Locke’s, 0.85% NaCl, and phosphate-buffered saline. Most amino acids in the media favored CsNEJ survival; however, aspartic and glutamic acids and adenine reduced survival. Survival was also significantly lower in media containing more than 0.1% bile. CsNEJs preconditioned in low bile media survived longer in higher bile media. All bile acids and conjugated bile salts were found to favor CsNEJ survival, except for lithocholic acid (LCA) which was toxic. NCTC 109 medium was found to be optimal for the in vitro maintenance of CsNEJs and 1× Locke’s solution to be suitable for analyzing the biological effects of bioactive compounds and molecules. Based on these results, we propose that bile acids enhance activity of CsNEJs, but LCA deteriorate CsNEJs.

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Acknowledgment

This work was supported by a grant R01-2006-000-10832-0 from the Basic Research Program of the Korea Science and Engineering Foundation.

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Authors and Affiliations

  1. Department of Medical Environmental Biology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul, 156-756, Republic of Korea

    Shunyu Li, Tae Im Kim, Won Gi Yoo & Sung-Jong Hong

  2. Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Centers for Disease control and Prevention, Seoul, 122-701, Republic of Korea

    Pyo Yun Cho

  3. Department of Parasitology, Inha University College of Medicine, Yonghyun-dong, Nam-gu Inchen, 402-751, Republic of Korea

    Tong-Soo Kim

  4. Department of Parasitology, Yanbian University College of Medicine, YanJi, Jilin Province, 133000, People’s Republic of China

    Shunyu Li

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  1. Shunyu Li
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  2. Tae Im Kim
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  3. Won Gi Yoo
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  4. Pyo Yun Cho
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Correspondence to Sung-Jong Hong.

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Li, S., Kim, T.I., Yoo, W.G. et al. Bile components and amino acids affect survival of the newly excysted juvenile Clonorchis sinensis in maintaining media. Parasitol Res 103, 1019–1024 (2008). https://doi.org/10.1007/s00436-008-1084-3

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  • DOI: https://doi.org/10.1007/s00436-008-1084-3

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