Abstract
Trichomoniasis is now an important health problem in developing countries. Although metronidazole has so far been widely used to treat this disease, the prevalence of metronidazole-resistant protozoa and unpleasant adverse effects have been found. In this study, natural products purified from Thai plants were, therefore, investigated for their effectiveness against Trichomonas vaginalis. The minimal inhibitory concentrations for all β-glycosides against Trichomonas vaginalis at 24 h were in a range of 6.25–12.5 μM. In addition, torvoside A and H were found to be more potent than their corresponding aglycones, deglucosylated torvoside A and H, while other β-glycosides were generally as active as their corresponding aglycones. The cytotoxicity of these compounds was also determined. Except for dalcochinin, none of the tested compounds showed cytotoxicity against Vero and cancer cell lines (KB and MCF-7), having IC50 values greater than 50 μg/ml. In conclusion, β-glycosides and several aglycones showed selective inhibition against Trichomonas vaginalis without harmful effect to mammalian cells.
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Acknowledgements
This work was financially supported by the Thailand Research Fund and the Commission on Higher Education grant (MRG # 4780002). J.S. is a Senior Research Scholar of the Thailand Research Fund. Thanks are due to Mr. Tanawat Phongsak and Miss Jeerapha Khowsukh for their help.
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Arthan, D., Sithiprom, S., Thima, K. et al. Inhibitory effects of Thai plants β-glycosides on Trichomonas vaginalis . Parasitol Res 103, 443–448 (2008). https://doi.org/10.1007/s00436-008-0996-2
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DOI: https://doi.org/10.1007/s00436-008-0996-2