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Contemporary identification of previously reported novel Cryptosporidium isolates reveals Cryptosporidium bovis and the cervine genotype in sheep (Ovis aries)

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Abstract

Molecular characterisation of Cryptosporidium spp. from sheep (Ovis aries), sampled during the investigation of a waterborne outbreak of human cryptosporidiosis caused by Cryptosporidium parvum, previously established that the sheep were not the source of infection. At the time, a novel Cryptosporidium genotype was identified at the Cryptosporidium oocyst wall protein locus in 26 isolates and a further ten isolates amplified with heat shock protein primers but remained unidentified. Subsequent investigation of small subunit ribosomal DNA has revealed that 22 samples contained Cryptosporidium cervine genotype, five contained Cryptosporidium bovis and four samples contained both the cervine genotype and C. bovis.

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References

  • Chalmers RM, Elwin K, Reilly WJ, Irvine H, Thomas AL, Hunter PR (2002) Cryptosporidium in farmed animals: the detection of a novel isolate in sheep. Int J Parasitol 32:21–26

    Article  PubMed  CAS  Google Scholar 

  • Elwin K, Chalmers RM, Roberts R, Guy EC, Casemore DP (2001) Modification of a rapid method for the identification of gene-specific polymorphisms in Cryptosporidium parvum and its application to clinical and epidemiological investigations. Appl Environ Microbiol 67:5581–5584

    Article  PubMed  CAS  Google Scholar 

  • Giles M, Chalmers RM, Pritchard G, Elwin K, Mueller-Doblies D, Clifton-Hadley FA (2008). Cryptosporidium hominis in a goat and sheep in the UK. Vet Rec (in press)

  • Learmonth JJ, Ionas G, Ebbett KA, Kwan ES (2004) Genetic characterisation and transmission cycles of Cryptosporidium species isolated from humans in New Zealand. Appl Environ Microbiol 70:3973–3978

    Article  PubMed  CAS  Google Scholar 

  • Leoni F, Amar C, Nichols G, Pedraza-Diaz S, McLaughlin J (2006) Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000. J Med Microbiol 55:703–707

    Article  PubMed  CAS  Google Scholar 

  • Ong CSL, Eisler DL, Alikhani A, Fung VWK, Tomblin J, Bowie WR, Issac-Renton JL (2002) Novel Cryptosporidium genotypes in sporadic cryptosporidiosis cases: First report of human infections with a cervine genotype. Emerg Infect Dis 5:1–6

    Google Scholar 

  • Qamruddin AO, Keaney MGL, McCann R, Chadwisk PR (2002) Increased stool sampling during a waterborne outbreak of cryptosporidiosis does not increase the detection of other faecal pathogens. J Clin Pathol 55:271–274

    PubMed  CAS  Google Scholar 

  • Rochelle PA, Ferguson DM, Handojo TJ, De Leon R, Stewart MH, Wolfe RL (1997) An assay combining cell culture with reverse transcriptase PCR to detect and differentiate the infectivity of waterborne Cryptosporidium parvum. Appl Environ Microbiol 63:2029–2037

    PubMed  CAS  Google Scholar 

  • Ryan UM, Bath C, Robertson I, Read C, Elliot A, Mcinnes L, Traub R, Besier B (2005) Sheep may not be an important zoonotic reservoir for Cryptosporidium and Giardia parasites. Appl Environ Microbiol 71:4992–4997

    Article  PubMed  CAS  Google Scholar 

  • Santin M, Trout JM, Fayer R (2007) Prevalence and molecular characterisation of Cryptosporidium and Giardia species and genotypes in sheep in Maryland. Vet Parasitol 146:17–24

    Article  PubMed  CAS  Google Scholar 

  • Santin M Fayer R (2007) Intragenotypic variations in the Cryptosporidium sp. Cervine genotype from sheep with implications for public health. J Parasitol 93:668–672

    Article  Google Scholar 

  • Soba B, Petrovec M, Mioc V, Logar J (2006) Molecular characterisation of Cryptosporidium isolates from humans in Slovenia. Clin Microbiol Infect 12:918–921

    Article  PubMed  CAS  Google Scholar 

  • Trotz-Williams LA, Martin DS, Gatei W, Cama V, Peregrine AS, Martin SW, Nydam DV, Jamieson F, Xiao L (2006) Genotype and subtype analyses of Cryptosporidium isolates from dairy calves and humans in Ontario. Parasitol Res 99:346–352

    Article  PubMed  CAS  Google Scholar 

  • Xiao L, Alderisio K, Limor J, Royer M, Lal AA (2000) Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool. Appl Environ Microbiol 66:5492–5498

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

We thank Guy Robinson and Stephen Hadfield (UK CRU) for helpful and stimulating discussions. The work described here was carried out in compliance with current UK legislation.

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Authors and Affiliations

  1. UK Cryptosporidium Reference Unit, National Public Health Service for Wales Microbiology Swansea, Singleton Hospital, Swansea, SA2 8QA, UK

    Kristin Elwin & Rachel M. Chalmers

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  1. Kristin Elwin
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  2. Rachel M. Chalmers
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Correspondence to Kristin Elwin.

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Elwin, K., Chalmers, R.M. Contemporary identification of previously reported novel Cryptosporidium isolates reveals Cryptosporidium bovis and the cervine genotype in sheep (Ovis aries). Parasitol Res 102, 1103–1105 (2008). https://doi.org/10.1007/s00436-008-0935-2

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  • DOI: https://doi.org/10.1007/s00436-008-0935-2

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