Abstract
The effect of gastrointestinal parasitism on patterns of edible tissue depletion of doramectin was studied in greyface Suffolk lambs. Twelve weight-matched pairs of lambs were allocated into group I (nonparasitized, pretreated with three administrations of 5 mg/kg fenbendazole) and group II (parasitized, did not receive anthelmintic treatment). Both groups were maintained together under similar conditions for 70 days, when they were treated with a subcutaneous dose of 0.2 mg/kg bw doramectin. At 7, 14, 21, and 28 days after doramectin administration, three lambs from each group were slaughtered and samples of liver, kidney, muscle, and fat were obtained. Pre-treatment with fenbendazole significantly reduced the nematode fecal egg count and significantly increased lamb body weight compared to the parasitized group. Doramectin was detected in all of the tissues up to 28 days post-treatment. Significantly higher and more persistent doramectin concentrations were found in the nonparasitized lambs compared to the parasitized animals. Considering the EMEA maximum residue limits for doramectin in fat, the calculated withdrawal period for the healthy lambs (43 days) was significantly higher than that for the parasitized animals (26 days).
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References
Aerts MML, Hogenboom AC, Brinkman UAR (1995) Analytical strategies for screening of veterinary drugs and their residues in edible products. J Chromatogr B 667:1–40
Atta AH, Abo-Shihada MN (2000) Comparative pharmacokinetics of doramectin and ivermectin in sheep. J Vet Pharmacol Ther 23:49–52
Baggot JD (1992) Disposition and fate of drugs in the body. In: Booth NH, McDonald LE (eds) Veterinary pharmacology and therapeutics. 6th edn. Iowa State University Press, Ames, pp 38–71
Barriga O (1996) Veterinary parasitology. Burgess International Group, Inc., Minneapolis MN, USA, pp 16.1–16.12
Bogan JA, McKellar Q (1988) The pharmacodynamic of ivermectin in sheep and cattle. J Vet Pharmacol Ther 11:260–268
Conder GA, Baker WJ (2002) Chemistry, pharmacology and safety: doramectin and selamectin. In: Vercruysse J, Rew RS (eds) Macrocyclic lactones in antiparasitic therapy. CAB International, Wallingford, UK, pp 30–50
Coop RL, Kyriazakis I (1999) Nutrition–parasite interaction. Vet Parasitol 84:187–204
Coop RL, Barger IA, Jackson F (2002) The use of macrocyclic lactones to control parasites of sheep and goats. In: Vercruysse J, Rew RS (eds) Macrocyclic lactones in antiparasitic therapy. CAB International, Wallingford, UK, pp 303–321
Dynes RA, Poppi DP, Barrel GK, Sykes AR (1998) Elevation of feed intake in parasite-infected lambs by central administration of a cholecystokinin receptor antagonist. Br J Nutr 79:47–54
EMEA (1996) Note for guidance: approach towards harmonisation of withdrawal periods. EMEA/CVMP/036/95. Final
EMEA (1999) Doramectin (extension to deer including reindeer). Summary report (4). EMEA/MRL/637/99-Final
Fox MT (1997) Pathophysiology of infection with gastrointestinal nematode in domestic ruminants: recent developments. Vet Parasitol 72:285–308
Gottschall DW (1997) A comparison of the pharmacokinetics and tissue residues of doramectin after intravenous and intramuscular administration in sheep. In: Proceedings of 16th International Conference of the World Association for the Advancement of Veterinary Parasitology, Sun City, South Africa, 13 August, pp 9–19
Hennessy D, Page SW, Gottschall DW (2000) The behaviour of doramectin in the gastrointestinal tract, its secretion in bile and pharmacokinetic disposition in the peripheral circulation after oral and intravenous administration to sheep. J Vet Pharmacol Ther 23:203–213
Hennessy DR, Alvinerie MR (2002) Pharmacokinetics of the macrocyclic lactones: conventional wisdom and new paradigm. In: Vercruysse J, Rew RS (eds) Macrocyclic lactones in antiparasitic therapy. CAB International, Wallingford, UK, pp 97–123
Holmes PH (1987) Pathophysiology of nematode infection. Int J Parasitol 17:433–451
Lanusse C, Gascon LH, Prichard RK (1995) Comparative plasma disposition of albendazole, fenbendazole, oxfendazole and their metabolites in adult sheep. J Vet Pharmacol Ther 18:196–203
Lanusse C, Lifschitz A, Virkel G, Alvarez L, Sánchez S, Sutra JF, Galtier P, Alvinerie M (1997) Comparative plasma disposition kinetic of ivermectin, moxidectin and doramectin in cattle. J Vet Pharmacol Ther 20:91–99
Lespine A, Sutra JF, Dupuy J, Alvinerie M, Aumont G (2004) The influence of parasitism on the pharmacokinetics of moxidectin in lambs. Parasitol Res 93:121–126
Lifschitz A, Murno G, Sallovitz J, Virkel G, Lanusse C (1997) Malnutrition modifies ivermectin disposition kinetics in calves. J Vet Pharmacol Ther 20(Suppl. 1):102–103
Lifschitz A, Imperiale F, Virkel G, Muñoz-Cobeñas M, Scherling N, DeLay R, Lanusse C (2000) Depletion of moxidectin tissue residues in sheep. J Agric Food Chem 48:6011–6015
Meyer D, Coles E, Rich L (1992) Veterinary laboratory medicine: interpretation and diagnosis. Saunders, Philadelphia PA, USA, p 331
Nowakowski M, Lynch M, Smith D, Logan N, Mouzin D, Lukaszewicz J, Ryan N, Hunter R, Jones R (1995) Pharmacokinetics and bioequivalence of parenterally administered doramectin in cattle. J Vet Pharmacol Ther 18:290–298
Okonkwo PO, Ogbuokiri JE, Ofoegbu E, Klotz U (1993) Protein binding and ivermectin estimations in patients with onchocerciasis. Clin Pharmacol Ther 53:426–430
Pérez R, Palma C, Araneda M, Cabezas I, Rubilar L, Arboix M (2007) Gastrointestinal parasitism reduces the plasma availability of doramectin in lambs. Vet J 173:169–175
Pérez R, Palma C, Cabezas I, Araneda M, Rubilar L, Alvinerie M (2006) Effect of parasitism on the pharmacokinetic disposition of ivermectin in lambs. J Vet Med 53:43–48
Poppi DP, MacRae JC, Brewer A, Coop RL (1986) Nitrogen transactions in digestive tract of lambs exposed to the intestinal parasite Trichostrongylus colubriformis. B J Nutr 55:593–602
Rohrer SP, Evans DV (1990) Binding characteristics of ivermectin in plasma from collie dogs. Vet Res Commun 14:157–165
Sklavounos C, Dmers N, Lukaszewicz J, Nowakowski M (1994) Photoisomerization of aromatic doramectin derivatives. J Agric Food Chem 42:1228–1231
Steel JW (1974) Pathophysiology of gastrointestinal nematode infections in the ruminant. Proc Aust Soc Anim Prod 10:139–147
Stromberg B, Vatthauer R, Schotthauer J, Myers G, Haggard D, King V, Hanke H (1997) Production responses following strategic parasite control in a beef cow/calf herd. Vet Parasitol 68:315–322
Sykes AR (1978) The effect of subclinical parasitism in sheep. Vet Rec 102:32–34
Vranic ML, Marangunich L, Fernandez Courel H, Fernandez Suarez A (2003) Estimation the withdrawal period for veterinary drug used in food producing animals. Anal Chim Acta 483:251–257
Wells RJ (2000) Do validation requirements for chemical methods in quantitative analysis need to be flexible? In: Proceeding of the 8th International Congress of EAVPT, Jerusalem, Israel, 30 July–3 August
Wicks SR, Kaye B, Weatherley AJ, Lewis D, Davidson E, Gibson SP, Smith DG (1993) Effect of formulation on the pharmacokinetics and efficacy of doramectin. Vet Parasitol 49:17–26
Zajac AM (1994) Fecal examination in the diagnosis of parasitism. In: Sloss MW, Kemp RL, Zajac AM (eds) Veterinary clinical parasitology. 6th edn. Iowa State University Press, Ames IA, USA, pp 3–93
Acknowledgments
We thank Pfizer Laboratories for providing the DRM analytical standard. This study was supported by FONDECYT Research Grant 1030609.
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Pérez, R., Palma, C., Nuñez, M.J. et al. Patterns of doramectin tissue residue depletion in parasitized vs nonparasitized lambs. Parasitol Res 102, 1051–1057 (2008). https://doi.org/10.1007/s00436-008-0874-y
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DOI: https://doi.org/10.1007/s00436-008-0874-y