Skip to main content
SpringerLink
Log in

In vitro evaluation of the effect of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense, and Pochonia chlamydosporia on Ascaris suum eggs

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

The in vitro effect of four isolates of the nematophagous fungi Duddingtonia flagrans (AC 001), Monacrosporium sinense (SF 53), and Pochonia chlamydosporia (VC 1 and VC 4) on eggs of Ascaris suum was evaluated. One hundred thousand A. suum eggs were plated on 2% water–agar with the grown isolates and control without fungus. After 7, 14, and 21 days, 100 eggs were removed and classified according to the following parameters: type 1, lytic effect without morphological damage to eggshell; type 2, lytic effect with morphological alteration of embryo and eggshell; and type 3, lytic effect with morphological alteration of embryo and eggshell, besides hyphal penetration and internal egg colonization. P. chlamydosporia showed ovicidal activity (p < 0.01), mainly of the type 3 effect, on A. suum eggs in the studied intervals of 13.3% (isolate VC 1) and 17.3% (isolate VC 4), 13.9% (VC 1) and 17.7% (VC 4), and 19% (VC 1) and 20% (VC4), respectively, at 7, 14, and 21 days. The other fungi showed no type 3 effect. P. chlamydosporia is a potential biological control agent of A. suum eggs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ayres M, Ayres JRM, Ayres DL, Santos AS (2003) Aplicações estatísticas nas áreas de ciências biológicas, Brasília

  • Araújo JV, Santos MA, Ferraz S (1995) Efeito ovicida de fungos nematófagos sobre ovos embrionados de Toxocara canis. Arq Bras Med Vet Zootec 47:37–42

    Google Scholar 

  • Araújo JV, Mota MA, Campos AK (2004a) Controle biológico de helmintos parasitos de animais por fungos nematófagos. Rev Bras Parasitol Vet 13:165–170

    Google Scholar 

  • Araújo JV, Guimarães MP, Campos AK (2004b) Control of bovine gastrointestinal nematodes parasites using pellets of the nematode trapping fungus Monacrosporium thaumasium. Ciênc Rural 34:457–463

    Google Scholar 

  • Araújo JV, Freitas BW, Vieira TC, Campos AK (2006a) Avaliação do fungo predador de nematóides Duddingtonia flagrans sobre larvas infectantes de Haemonchus contortus e Strongyloides papillosus de caprinos. Rev Bras Parasitol Vet 15:76–79

    PubMed  Google Scholar 

  • Araújo JV, Assis RCL, Campos AK, Mota MA (2006b) Efeito antagônico de fungos predadores dos gêneros Monacrosporium, Arthrobotrys e Duddingtonia sobre larvas infectantes de Cooperia sp. e Oesophagostomum sp. Arq Bras Med Vet Zootec 58:373–380

    Article  Google Scholar 

  • Braga FR, Araújo JV, Campos AK, Carvalho RO, Silva AR, Tavela AO, Maciel AS (2007) Observação in vitro da ação dos isolados fúngicos Duddingtonia flagrans, Monacrosporium thaumasium e Verticillium chlamydosporium sobre ovos de Ascaris lumbricoides (Lineu, 1758). Rev Soc Bras Med Trop 40:356–358

    Article  PubMed  Google Scholar 

  • Campos AK, Mota MA, Araújo JV, Cecon PR (2004) Atividade predatória, crescimento radial e esporulação e fungos predadores de nematóides Monacrosporium spp, submetidos à criopreservação. Ciênc Rural 24:465–469

    Google Scholar 

  • Campos AK, Araújo JV, Assis RCL, Grandra JR, Guimarães MP (2007) Viabilidade de formulação peletizada do fungo nematófago Monacrosporium sinense, no controle biológico de nematóides parasitos gastrintestinais de bezerros. Arq Bras Med Vet Zootec 59:14–20

    Google Scholar 

  • Castro AA, Oliveira CRC, Anjos DHS, Ornellas EI, Bittencourt REP, Araújo JV, Sampaio IBM, Rodrigues MLA (2003) Potencial dos fungos nematófagos Arthrobotrys sp. e Monacrosporium thaumasium para o controle de larvas de ciatostomíneos de eqüinos (Nematoda: Cyathostominae). Rev Bras Parasitol Vet 12:49–53

    Google Scholar 

  • Chandrawathani P, Jamnah O, Waller PJ, Larsen M, Gillespie AT, Zahari WM (2003) Biological control of nematode parasites of small ruminants in Malaysia using the nematophagous fungus Duddingtonia flagrans. Vet Parasitol 117:173–183

    Article  PubMed  CAS  Google Scholar 

  • Dimander SO, Höglund J, Uggla A, Spörndly E, Waller PJ (2003) Evaluation of gastro-intestinal nematode parasite control strategies for first-season grazing cattle in Sweden. Vet Parasitol 111:192–209

    Article  Google Scholar 

  • Fontenot ME, Miller JE, Peña MT, Larsen M, Gillespie A (2003) Efficiency of feeding Duddingtonia flagrans chlamydospores to grazing ewes on reducing availability of parasitic nematode larvae on pasture. Vet Parasitol 118:203–213

    Article  PubMed  CAS  Google Scholar 

  • Gams W, Zare R (2001) A revision of Verticillium sect. Prostrata. III. Generic classification. Nova Hedwig 73:329–337

    Google Scholar 

  • Gomes AS, Araújo JV, Ribeiro RCF (1999) Differential in vitro pathogenicity of predatory fungi of the genus Monascrosporium for phytonematodes, free-living nematode and parasitic nematodes of cattle. Braz J Med Biol Res 32:79–83

    PubMed  CAS  Google Scholar 

  • Kahn LP, Norman TM, Walkden-Brown SW, Crampton A, O’connor LJ (2007) Trapping efficacy of Duddingtonia flagrans against Haemonchus contortus at temperatures existing at lambing in Australia. Vet Parasitol 146:83–89

    Article  PubMed  CAS  Google Scholar 

  • Larsen M (1999) Biological control of helminths. Int J Parasitol 29:139–146

    Article  PubMed  CAS  Google Scholar 

  • Lysek H (1976) Classification of ovicide fungi according to type of ovicidity. Acta University Palacki Olumue 76:9–13

    Google Scholar 

  • Lysek H (1978) A scanning electron microscope study of the effects of an ovicidal fungus on the eggs of Ascaris lumbricoides. Parasitol 77:139–141

    Article  Google Scholar 

  • Lysek H, Sterba J (1991) Colonization of Ascaris lumbricoides eggs by the fungus Verticillium chlamydosporium Goddard. Folia Parasitol 38:255–259

    Article  PubMed  CAS  Google Scholar 

  • Lysek H, Fassatiová O, Pineda NC, Hernández NL (1982) Ovicidal fungi in soils of Cuba. Folia Parasitol 29:265–270

    PubMed  CAS  Google Scholar 

  • Mizobutsi E, Ferraz S, Ribeiro RCF (2000) Avaliação do parasitismo de diversos isolados fúngicos em ovos de Heterodera glycines e Meloidogyne javanica. Nematol Bras 24:167–172

    Google Scholar 

  • Morgan-Jones G, White JF, Rodríguez-Kábana R (1983) Phytonematode pathology: ultrastuctural studies. Parasitism of Meloidogyne arenaria eggs by Verticillium chlamydosporium. Nematropica 13:245–260

    Google Scholar 

  • Mota MA, Campos AK, Araújo JV (2003) Controle biológico de helmintos parasitos de animais: estágio atual e perspectivas futuras. Pesq Vet Bras 23:93–100

    Article  Google Scholar 

  • Roepstorff A, Nansen P (1994) Epidemiology and control of helminth infections in pigs under intensive and nonintensive production systems. Vet Parasitol 54:69–85

    Article  PubMed  CAS  Google Scholar 

  • Roppa L (1998) Suinocultura brasileira. Suin Ind 20:24–32

    Google Scholar 

  • Stirling GR, West LM (1991) Fungal parasites of root-knot nematodes eggs from tropical and sub tropical regions of Australia. Australas Plant Pathol 20:149–154

    Article  Google Scholar 

  • Urquhart GM, Armour J, Duncan JL, Dunn AM, Jennings FW (1998) Parasitologia Veterinária. 2° Ed., Editora Guanabara Koogan, Rio de Janeiro, p. 273

  • Waghorn TS, Leathwick DM, Chen LY, Skipp RA (2003) Efficacy of the nematode-trapping fungus Duddingtonia flagrans against three species of gastro-intestinal nematodes in laboratory faecal cultures from sheep and goats. Vet Parasitol 118:227–234

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    J. V. Araújo, F. R. Braga, A. R. Silva, J. M. Araujo & A. O. Tavela

  2. CNPq, Rio de Janeiro, Brazil

    J. V. Araújo

Authors
  1. J. V. Araújo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. R. Braga
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. R. Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. M. Araujo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. O. Tavela
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. V. Araújo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Araújo, J.V., Braga, F.R., Silva, A.R. et al. In vitro evaluation of the effect of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense, and Pochonia chlamydosporia on Ascaris suum eggs. Parasitol Res 102, 787–790 (2008). https://doi.org/10.1007/s00436-007-0852-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-007-0852-9

Keywords

Search

Navigation