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Multilocus phylogenetic analysis of Cryptosporidium andersoni (Apicomplexa) isolated from a bactrian camel (Camelus bactrianus) in China

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Abstract

This is the first report of cryptosporidiosis in a bactrian camel (Camelus bactrianus) in China. Two Cryptosporidium isolates derived from the same bactrian camel (3-year-old) in November 2005 and April 2006 were characterized using sequence and phylogenetic analysis of the small-subunit rRNA (18S rRNA), 70-kDa heat shock protein (HSP70), actin and Cryptosporidium oocyst wall protein (COWP) genes. The sequences of the 18S rRNA and COWP were identical to all other Cryptosporidium andersoni isolates although minor differences were noticed between the isolates and the USA isolate at the actin locus (99.2% of similarity). The sequence of the HSP70 was identical to the Japanese C. andersoni isolate, with a minor difference from the Australian C. andersoni isolate (99.7% of similarity). Cross-transmission studies demonstrated that the C. andersoni isolates did not infect immunosuppressed or immunocompetent Kun-ming mice, severe combined immunodeficiency mice, and immunosuppressed or immunocompetent calves. Among the C. andersoni isolates reported so far, only isolates from Japan could infect SCID mice. Thus, the C. andersoni isolates from the bactrian camel were biologically similar to most bovine C. andersoni isolates characterized so far, but are different from bovine isolates from Japan.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (number 30371079) and Henan Innovation Project for University Prominent Research Talents (number 2004KYCX002) and the Program for New Century Excellent Talents in University (number NCET-05-0382). We gratefully thank Professor R. B. Williams, the Coxitec Consulting, United Kingdom, for his critical reading of the manuscript.

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Authors and Affiliations

  1. The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, the People’s Republic of China

    Rongjun Wang, Longxian Zhang, Changshen Ning, Fuchun Jian, Biao Lu & Heping Dong

  2. National Institute for Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, 200025, the People’s Republic of China

    Yaoyu Feng

  3. Division of Parasitic Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, GA, 30341, USA

    Lihua Xiao

  4. The Wild Animals Rescue Center of Henan Province, Zhengzhou, 450044, the People’s Republic of China

    Weichang Ai

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  1. Rongjun Wang
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  2. Longxian Zhang
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  3. Changshen Ning
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  4. Yaoyu Feng
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  7. Biao Lu
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  8. Weichang Ai
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  9. Heping Dong
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Correspondence to Longxian Zhang.

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Wang, R., Zhang, L., Ning, C. et al. Multilocus phylogenetic analysis of Cryptosporidium andersoni (Apicomplexa) isolated from a bactrian camel (Camelus bactrianus) in China. Parasitol Res 102, 915–920 (2008). https://doi.org/10.1007/s00436-007-0851-x

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  • DOI: https://doi.org/10.1007/s00436-007-0851-x

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